Construct: ORF TRCN0000471447
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017602.1_s317c1
- Derived from:
- ccsbBroadEn_13910
- DNA Barcode:
- TTAAGTCTTAATGGCGCGCGCACT
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NDUFA5 (4698)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471447
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4698 | NDUFA5 | NADH:ubiquinone oxidoreduct... | NM_005000.5 | 99.7% | 99.1% | 345delA |
| 2 | human | 4698 | NDUFA5 | NADH:ubiquinone oxidoreduct... | NM_001282421.2 | 95.9% | 93.1% | (many diffs) |
| 3 | human | 4698 | NDUFA5 | NADH:ubiquinone oxidoreduct... | NM_001282420.2 | 95.7% | 93.9% | (many diffs) |
| 4 | human | 4698 | NDUFA5 | NADH:ubiquinone oxidoreduct... | NM_001282419.2 | 86.7% | 86.2% | 19_20ins45;300delA |
| 5 | human | 4698 | NDUFA5 | NADH:ubiquinone oxidoreduct... | NM_001282422.2 | 66% | 65.5% | 65_66ins117;228delA |
| 6 | human | 4698 | NDUFA5 | NADH:ubiquinone oxidoreduct... | NM_001291304.1 | 56.6% | 51.8% | (many diffs) |
| 7 | human | 4698 | NDUFA5 | NADH:ubiquinone oxidoreduct... | XM_024446771.1 | 50.5% | 50% | 0_1ins171;174delA |
| 8 | human | 4698 | NDUFA5 | NADH:ubiquinone oxidoreduct... | NR_111925.1 | 6.1% | (many diffs) | |
| 9 | human | 4698 | NDUFA5 | NADH:ubiquinone oxidoreduct... | NR_104169.2 | 6% | (many diffs) | |
| 10 | human | 4698 | NDUFA5 | NADH:ubiquinone oxidoreduct... | NR_104168.2 | 5.9% | (many diffs) | |
| 11 | human | 4698 | NDUFA5 | NADH:ubiquinone oxidoreduct... | NR_111926.1 | 5.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 414
- ORF length:
- 348
- Sequence:
-
1 tcTTCCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGGC GGGTGTGCTG AAGAAGACCA CTGGCCTTGT GGGATTGGCT GTGTGCAATA 121 CTCCTCACGA GAGGCTAAGA ATATTGTACA CAAAGATTCT TGATGTTCTT GAGGAAATCC 181 CTAAAAATGC AGCATATAGA AAGTATACAG AACAGATTAC AAATGAGAAG CTGGCTATGG 241 TTAAAGCGGA ACCAGATGTT AAAAAATTAG AAGACCAACT TCAAGGCGGT CAATTAGAAG 301 AGGTGATTCT TCAGGCTGAA CATGAACTAA ATCTGGCAAG AAAAATGAGG GAATGGAAAC 361 TATGGGAGCC ATTAGTGGAA GAGCCTCCTG CCGATCAGTG GAAATGGCCA TATACCCAAC 421 TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA 481 TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT 541 ATCTTGTGGA AAGGACGATT AAGTCTTAAT GGCGCGCGCA CTACGCGTTA AGTCgacaat 601 caacctctgg attacaaaat ttgtgaaaga tt