Construct: ORF TRCN0000471465
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010648.1_s317c1
- Derived from:
- ccsbBroadEn_12623
- DNA Barcode:
- GACGCACCCTGTTGTAATCACGAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PTCD2 (79810)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471465
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79810 | PTCD2 | pentatricopeptide repeat do... | XM_005248603.3 | 100% | 100% | |
| 2 | human | 79810 | PTCD2 | pentatricopeptide repeat do... | XM_017009866.2 | 91.9% | 91.9% | 0_1ins57 |
| 3 | human | 79810 | PTCD2 | pentatricopeptide repeat do... | NM_001284404.1 | 89% | 87.7% | (many diffs) |
| 4 | human | 79810 | PTCD2 | pentatricopeptide repeat do... | NM_001284405.1 | 83.8% | 83.8% | 0_1ins114 |
| 5 | human | 79810 | PTCD2 | pentatricopeptide repeat do... | XM_017009867.2 | 83.8% | 83.8% | 0_1ins114 |
| 6 | human | 79810 | PTCD2 | pentatricopeptide repeat do... | XM_017009868.1 | 83.8% | 83.8% | 372_373ins114 |
| 7 | human | 79810 | PTCD2 | pentatricopeptide repeat do... | NM_001284403.2 | 81% | 73.6% | (many diffs) |
| 8 | human | 79810 | PTCD2 | pentatricopeptide repeat do... | NM_024754.5 | 60.8% | 60.8% | 1_456del |
| 9 | human | 79810 | PTCD2 | pentatricopeptide repeat do... | XM_005248601.2 | 51% | 51% | 1_456del;828_829ins114 |
| 10 | human | 79810 | PTCD2 | pentatricopeptide repeat do... | XR_948298.2 | 40.4% | 1_466del;1175_1750del | |
| 11 | human | 79810 | PTCD2 | pentatricopeptide repeat do... | XR_241790.5 | 32.2% | 1_466del;1175_2195del | |
| 12 | human | 79810 | PTCD2 | pentatricopeptide repeat do... | XR_001742270.2 | 6.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 774
- ORF length:
- 708
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa agaccagcat ttacgaggtt tcttctcaga ctccacatca ttcaatattt 121 tgatggatat gttatttatc aaaggcaaat ataaaagtgc tttgcaagta ttgatagaga 181 tgaaaaacca agatgtgaag ttcaccaaag atacctatgt tcttgctttt gcaatttgct 241 acaaactgaa tagccctGAG TCTTTCAAAA TCTGTACTAC ATTAAGAGAA GAAGCTCTAC 301 TCAAAGGAGA AATTCTCTCC AGGAGAGCAT CCTGTTTCGC TGTGGCATTA GCTCTGAATC 361 AGAATGAGAT GGCAAAAGCT GTGTCCATTT TTTCTCAAAT CATGAATCCA GAAAGCATAG 421 CCTGCATTAA TTTAAATATT ATAATCCATA TCCAGTCAAA TATGTTGGAA AACCTGATAA 481 AGACTCTAAA AAATGCTGCA GAAGGAAATT TATCAAAATT TGTGAAAAGA CATGTGTTCT 541 CGGAGGAAGT GCTGGCCAAA GTGAGGGAAA AAGTGAAGGA TGTGCCTGCC CTTGTGGCCA 601 AATTTGATGA GATCTATGGG ACACTGCACA TCACTGGCCA GGTCACCACT GATTCTTTGG 661 ATGCTGTGCT CTGCCACACC CCCAGGGACA GGAAATCTCA CACGTTGCTA TTAAACAAGA 721 GGATGGTCAG CCGTCGCACC TTCCAGCCAC TCAGCCAGTC CCTGTTGGCT GAGTACCCAA 781 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 841 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 901 TATCTTGTGG AAAGGACGAG ACGCACCCTG TTGTAATCAC GAGACGCGTT AAGTCgacaa 961 tcaacctctg gattacaaaa tttgtgaaag att