Construct: ORF TRCN0000471475
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008785.1_s317c1
- Derived from:
- ccsbBroadEn_03202
- DNA Barcode:
- GAGGTCGCACTGGCTGGATGGCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ADIPOR1 (51094)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471475
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51094 | ADIPOR1 | adiponectin receptor 1 | NM_001290553.1 | 100% | 100% | |
| 2 | human | 51094 | ADIPOR1 | adiponectin receptor 1 | NM_001290557.1 | 100% | 100% | |
| 3 | human | 51094 | ADIPOR1 | adiponectin receptor 1 | NM_001290629.1 | 100% | 100% | |
| 4 | human | 51094 | ADIPOR1 | adiponectin receptor 1 | NM_015999.6 | 100% | 100% | |
| 5 | human | 51094 | ADIPOR1 | adiponectin receptor 1 | XM_017001407.1 | 58.6% | 58.6% | 0_1ins465 |
| 6 | human | 51094 | ADIPOR1 | adiponectin receptor 1 | XM_024447427.1 | 58.6% | 58.6% | 0_1ins465 |
| 7 | mouse | 72674 | Adipor1 | adiponectin receptor 1 | NM_001306069.1 | 90.9% | 96.8% | (many diffs) |
| 8 | mouse | 72674 | Adipor1 | adiponectin receptor 1 | NM_028320.4 | 90.9% | 96.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1191
- ORF length:
- 1125
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttagcatgtc ttcccacaaa ggatctgtgg tggcacaggg gaatggggct cctgccagta 121 acagggaagc tgacacggtg gaactggctg aactgggacc cctgctagaa gagaagggca 181 aacgggtaat cgccaaccca cccaaagctg aagaagagca aacatgccca gtgccccagg 241 aagaagagga ggaggtgcgg gtactgacac ttcccctgca agcccaccac gccatggaga 301 agatggaaga gtttgtgtac aaggtctggg agggacgttg gagggtcatc ccatatgatg 361 tgctccctga ctggctaaag gacaacgact atctgctaca tggtcataga cctcccatgc 421 cctcctttcg ggcttgcttc aagagcatct tccgcattca tacagaaact ggcaacatct 481 ggacccatct gcttggtttc gtgctgtttc tctttttggg aatcttgacc atgctcagac 541 caaatatgta cttcatggcc cctctacagg agaaggtggt ttttgggatg ttctttttgg 601 gtgcagtgct ctgcctcagc ttctcctggc tctttcacac cgtctattgt cattcagaga 661 aagTCTCTCG GACTTTTTCC AAACTGGACT ATTCAGGGAT TGCTCTTCTA ATTATGGGGA 721 GCTTTGTCCC CTGGCTCTAT TATTCCTTCT ACTGCTCCCC ACAGCCACGG CTCATCTACC 781 TCTCCATCGT CTGTGTCCTG GGCATTTCTG CCATCATTGT GGCGCAGTGG GACCGGTTTG 841 CCACTCCTAA GCACCGGCAG ACAAGAGCAG GCGTGTTCCT GGGACTTGGC TTGAGTGGCG 901 TCGTGCCCAC CATGCACTTT ACTATCGCTG AGGGCTTTGT CAAGGCCACC ACAGTGGGCC 961 AGATGGGCTG GTTCTTCCTC ATGGCTGTGA TGTACATCAC TGGAGCTGGC CTTTATGCTG 1021 CTCGAATTCC TGAGCGCTTC TTTCCTGGAA AATTTGACAT ATGGTTCCAG TCTCATCAGA 1081 TTTTCCATGT CCTGGTGGTG GCAGCAGCCT TTGTCCACTT CTATGGAGTC TCCAACCTTC 1141 AGGAATTCCG TTACGGCCTA GAAGGCGGCT GTACTGATGA CACCCTTCTC TGCCCAACTT 1201 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1261 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1321 CTTGTGGAAA GGACGAGAGG TCGCACTGGC TGGATGGCCA ACGCGTTAAG TCgacaatca 1381 acctctggat tacaaaattt gtgaaagatt