Construct: ORF TRCN0000471481
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015274.1_s317c1
- Derived from:
- ccsbBroadEn_10218
- DNA Barcode:
- TCAATAGCTGAGAGTGAGCAAACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SH3D19 (152503)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471481
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 152503 | SH3D19 | SH3 domain containing 19 | NM_001128923.2 | 99.6% | 99.6% | 1_9delATGAACATT |
| 2 | human | 152503 | SH3D19 | SH3 domain containing 19 | NM_001243349.2 | 99.6% | 99.6% | 1_9delATGAACATT |
| 3 | human | 152503 | SH3D19 | SH3 domain containing 19 | XM_017007782.1 | 99.6% | 99.6% | 1_9delATGAACATT |
| 4 | human | 152503 | SH3D19 | SH3 domain containing 19 | XM_017007783.2 | 99.6% | 99.6% | 1_9delATGAACATT |
| 5 | human | 152503 | SH3D19 | SH3 domain containing 19 | XM_024453903.1 | 99.6% | 99.6% | 1_9delATGAACATT |
| 6 | human | 152503 | SH3D19 | SH3 domain containing 19 | XM_024453905.1 | 99.6% | 99.6% | 1_9delATGAACATT |
| 7 | human | 152503 | SH3D19 | SH3 domain containing 19 | NM_001009555.3 | 96.7% | 96.7% | 1_9delATGAACATT;1242_1310del |
| 8 | human | 152503 | SH3D19 | SH3 domain containing 19 | XM_005262767.3 | 96.7% | 96.7% | 1_9delATGAACATT;1242_1310del |
| 9 | human | 152503 | SH3D19 | SH3 domain containing 19 | XM_011531646.2 | 96.7% | 96.7% | 1_9delATGAACATT;1242_1310del |
| 10 | human | 152503 | SH3D19 | SH3 domain containing 19 | XM_017007781.1 | 96.7% | 96.7% | 1_9delATGAACATT;1242_1310del |
| 11 | human | 152503 | SH3D19 | SH3 domain containing 19 | XM_024453901.1 | 96.7% | 96.7% | 1_9delATGAACATT;1242_1310del |
| 12 | human | 152503 | SH3D19 | SH3 domain containing 19 | XM_024453906.1 | 96.7% | 96.7% | 1_9delATGAACATT;1242_1310del |
| 13 | human | 152503 | SH3D19 | SH3 domain containing 19 | NM_001128924.1 | 94.9% | 94.7% | 1_9delATGAACATT;694_695ins108 |
| 14 | human | 152503 | SH3D19 | SH3 domain containing 19 | XM_017007779.2 | 94.9% | 94.7% | 1_9delATGAACATT;694_695ins108 |
| 15 | human | 152503 | SH3D19 | SH3 domain containing 19 | XM_017007780.1 | 77.3% | 77.3% | 1_672del |
| 16 | human | 152503 | SH3D19 | SH3 domain containing 19 | XM_017007778.1 | 75.5% | 75.5% | 1_672del;1905_1973del |
| 17 | human | 152503 | SH3D19 | SH3 domain containing 19 | XM_011531648.1 | 75.3% | 75.3% | 1_681del;1914_1982del |
| 18 | human | 152503 | SH3D19 | SH3 domain containing 19 | XM_024453904.1 | 75.3% | 75.3% | 1_681del;1914_1982del |
| 19 | human | 152503 | SH3D19 | SH3 domain containing 19 | XM_024453902.1 | 72.7% | 72.7% | 1_858del |
| 20 | human | 152503 | SH3D19 | SH3 domain containing 19 | XM_024453900.1 | 71.2% | 71.2% | 1_858del;2091_2159del |
| 21 | human | 152503 | SH3D19 | SH3 domain containing 19 | XM_011531649.2 | 69.3% | 69.2% | 1_858del;1543_1544ins108 |
| 22 | human | 152503 | SH3D19 | SH3 domain containing 19 | XM_011531647.2 | 67.8% | 67.7% | 1_858del;1543_1544ins108;1983_2051del |
| 23 | human | 152503 | SH3D19 | SH3 domain containing 19 | XM_024453907.1 | 22.8% | 22.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2361
- ORF length:
- 2292
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaacacagaa caaagccaaa atagtattgt ttccagaatt aaagtgtttg 121 agggtcagac aaacatagaa acctcaggac tgcccaagaa accagaaatt actccacgtt 181 cacttcctcc aaagcctact gtttcctcag ggaaaccttc tgtagctccc aaaccagctg 241 ctaacagagc ttctggagag tgggactctg ggactgagaa cagactcaag gtgacctcca 301 aggaaggact caccccatac cctcccctgc aagaagcggg aagcatccca gtaaccaaac 361 ctgaattgcc aaagaaacca aaccctggcc ttatacgaag tgttaatcct gagattccgg 421 gaagagggcc cctggctgag agctctgata gtgggaagaa agtgccaact cctgccccgc 481 ggcctttgct gctgaagaaa tctgtttcct cagaaaaccc cacctaccct tcagctccac 541 tgaaacctgt cactgttcct ccccgactcg caggggcatc acaagccaaa gcatacaagt 601 cactgggaga agggccccca gccaaccccc cagttccagt tctgcagagc aagcccttgg 661 tggacatcga tctcatcagc tttgatgatg atgttttgcc caccccatcg gggaacctgg 721 ctgaagaatc tgttggttca gagatggttc tagatccctt tcagctccct gcaaaaacag 781 aaccaataaa agaacgagca gttcaaccag cacccaccag gaagcccact gtaattcgaa 841 ttccagccaa accaggaaaa tgtttacatg aggatccaca aagtccacct cctctccctg 901 ctgaaaaacc tattggaaac actttcagta cagtatctgg aaagctcagt aatgttgaga 961 gaactagaaa cttggaatcc aaccacccag gtcaaacagg aggttttgtg cgagtacccc 1021 caaggttgcc accgagacct gtgaatggaa aaaccattcc aactcaacag cctccaacca 1081 aggtgccccc tgagagacca cctcccccaa agctttctgc aaccagaaga tctaataaga 1141 aactgccttt taatcgatcc tcttctgaca tggatcttca gaaaaaacaa agtaacttgg 1201 caactggact ctcaaaagcc aagagtcaag tttttaaaaa tcaagatccg gtgctacccc 1261 ctcgtcccaa accaggacac cctctctaca gtaaatacat gcgtggggat gtacttgtga 1321 tgctgaagca gacggaaaat aattacttgg agtgccaaaa gggagaagac actggcagag 1381 ttcacctgtc tcaaatgaag attatcactc cacttgatga acatcttaga agcagaccaa 1441 acgatccaag ccacgctcag aagcctgttg acagtggtgc tcctcatgct gtcgttcttc 1501 atgatttccc agcagagcaa gttgatgatt tgaacctcac ttctggagaa attgtttatc 1561 ttctggagaa gatagataca gattggtaca gagggaactg tagaaaccag attggcatat 1621 ttcctgccaa ctatgtcaaa gtgattattg atatcccaga aggaggaaat gggaaaagag 1681 aatgtgtttc atctcattgt gttaaaggct caagatgtgt tgctcggttt gaatatattg 1741 gagagcagaa ggatgagttg agtttctcag agggagaaat tattattctt aaagagtatg 1801 tgaatgagga atgggccaga ggagaagttc gaggcagaac tgggattttc cccctgaact 1861 ttgtggagcc tgttgaggat tatcccaccT CTGGTGCAAA TGTTTTAAGC ACAAAGGTAC 1921 CACTGAAAAC CAAAAAAGAA GATTCTGGCT CAAACTCTCA GGTTAACAGT CTTCCGGCAG 1981 AATGGTGTGA AGCTCTTCAC AGTTTTACAG CAGAGACCAG TGATGACTTA TCATTCAAGA 2041 GGGGAGACCG GATCCAGATT CTGGAACGTC TGGATTCTGA CTGGTGCAGG GGCAGACTGC 2101 AGGACAGGGA GGGGATCTTC CCAGCAGTGT TTGTGAGGCC CTGCCCAGCT GAGGCAAAAA 2161 GTATGTTGGC CATAGTACCG AAGGGGAGGA AGGCCAAAGC CTTATATGAT TTCCGAGGGG 2221 AGAATGAAGA TGAACTTTCC TTCAAGGCTG GAGATATAAT AACAGAGCTG GAATCTGTAG 2281 ATGATGACTG GATGAGTGGA GAACTTATGG GAAAATCTGG AATATTTCCC AAAAACTACA 2341 TACAGTTTCT ACAGATCAGC TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 2401 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 2461 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATCAA TAGCTGAGAG 2521 TGAGCAAACA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt