Construct: ORF TRCN0000471492
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001348.1_s317c1
- Derived from:
- ccsbBroadEn_02166
- DNA Barcode:
- AGAACTTATATGCAACAGCTCAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LY86 (9450)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471492
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9450 | LY86 | lymphocyte antigen 86 | NM_004271.4 | 100% | 100% | |
| 2 | human | 9450 | LY86 | lymphocyte antigen 86 | XM_017011505.1 | 73.8% | 62.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 552
- ORF length:
- 486
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gggtttcaca gccactctct tcctctggac tcTGATTTTT CCCAGCTGCA 121 GTGGAGGCGG CGGTGGGAAA GCCTGGCCCA CACACGTGGT CTGTAGCGAC AGCGGCTTGG 181 AAGTGCTCTA CCAGAGTTGC GATCCATTAC AAGATTTTGG CTTTTCTGTT GAAAAGTGTT 241 CCAAGCAATT AAAATCAAAT ATCAACATTA GATTTGGAAT TATTCTGAGA GAGGACATCA 301 AAGAGCTTTT TCTTGACCTA GCTCTCATGT CTCAAGGCTC ATCTGTTTTG AATTTCTCCT 361 ATCCCATCTG TGAGGCGGCT CTGCCCAAGT TTTCTTTCTG TGGAAGAAGG AAAGGAGAGC 421 AGATTTACTA TGCTGGGCCT GTCAATAATC CTGAATTTAC TATTCCTCAG GGAGAATACC 481 AGGTTTTGCT GGAACTGTAC ACTGAAAAAC GGTCCACCGT GGCCTGTGCC AATGCTACTA 541 TCATGTGCTC CTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 601 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 661 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAAGA ACTTATATGC AACAGCTCAA 721 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t