Construct: ORF TRCN0000471503
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007280.1_s317c1
- Derived from:
- ccsbBroadEn_03481
- DNA Barcode:
- TTTCTGAACCGCCCTTAATAACAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DUS2 (54920)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471503
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54920 | DUS2 | dihydrouridine synthase 2 | NM_001271762.2 | 100% | 100% | |
| 2 | human | 54920 | DUS2 | dihydrouridine synthase 2 | NM_017803.5 | 100% | 100% | |
| 3 | human | 54920 | DUS2 | dihydrouridine synthase 2 | NM_001271763.2 | 92.9% | 92.9% | 263_264ins105 |
| 4 | mouse | 66369 | Dus2 | dihydrouridine synthase 2 | NM_025518.4 | 88.2% | 90.2% | (many diffs) |
| 5 | mouse | 66369 | Dus2 | dihydrouridine synthase 2 | XM_006531278.3 | 88.2% | 90.2% | (many diffs) |
| 6 | mouse | 66369 | Dus2 | dihydrouridine synthase 2 | NM_001301176.1 | 67.4% | 67.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1545
- ORF length:
- 1479
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat tttgaatagc ctctctctgt gttaccataa taagctaatc ctggccccaa 121 tggttcgggt agggactctt ccaatgaggc tgctggccct ggattatgga gcggacattg 181 tttactgtga ggagctgatc gacctcaaga tgattcagtg caagagagtt gttaatgagg 241 tgctcagcac agtggacttt gtcgcccctg atgatcgagt tgtcttccgc acctgtgaaa 301 gagagcagaa cagggtggtc ttccagatgg ggacttcaga cgcagagcga gcccttgctg 361 tggccaggct tgtagaaaat gatgtggctg gtattgatgt caacatgggc tgtccaaaac 421 aatattccac caagggagga atgggagctg ccctgctgtc agaccctgac aagattgaga 481 agatcctcag cactcttgtt aaagggacac gcagacctgt gacctgcaag attcgcatcc 541 tgccatcgct agaagatacc ctgagccttg tgaagcggat agagaggact ggcattgctg 601 ccatcgcagt tcatgggagg aagcgggagg agcgacctca gcatcctgtc agctgtgaag 661 tcatcaaagc cattgctgat accctctcca ttcctgtcat agccaacgga ggatctcatg 721 accacatcca acagtattcg gacatagagg actttcgaca agccacggca gcctcttccg 781 tgatggtggc ccgagcagcc atgtggaacc catctatctt cctcaaggag ggtctgcggc 841 ccctggagga ggtcatgcag aaatacatca gatacgcggt gcagtatgac aaccactaca 901 ccaacaccaa gtactgcttg tgccagatgc tacgagaaca gctggagtcg ccccagggaa 961 ggttgctcca tgctgcccag tcttcccggg aaatttgtga ggcctttggc cttggtgccT 1021 TCTATGAGGA GACCACACAG GAGCTGGATG CCCAGCAGGC CAGGCTCTCA GCCAAGACTT 1081 CAGAGCAGAC AGGGGAGCCA GCTGAAGATA CCTCTGGTGT CATTAAGATG GCTGTCAAGT 1141 TTGACCGGAG AGCATACCCA GCCCAGATCA CCCCTAAGAT GTGCCTACTA GAGTGGTGCC 1201 GGAGGGAGAA GTTGGCACAG CCTGTGTATG AAACGGTTCA ACGCCCTCTA GATCGCCTGT 1261 TCTCCTCTAT TGTCACCGTT GCTGAACAAA AGTATCAGTC TACCTTGTGG GACAAGTCCA 1321 AGAAACTGGC GGAGCAGGCT GCAGCCATCG TCTGTCTGCG GAGCCAGGGC CTCCCTGAGG 1381 GTCGGCTGGG TGAGGAGAGC CCTTCCTTGC ACAAGCGAAA GAGGGAGGCT CCTGACCAAG 1441 ACCCTGGGGG CCCCAGAGCT CAGGAGCTAG CACAACCTGG GGATCTGTGC AAGAAGCCCT 1501 TTGTGGCCTT GGGAAGTGGT GAAGAAAGCC CCCTGGAAGG CTGGTACCCA ACTTTCTTGT 1561 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1621 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1681 GAAAGGACGA TTTCTGAACC GCCCTTAATA ACAAACGCGT TAAGTCgaca atcaacctct 1741 ggattacaaa atttgtgaaa gatt