Construct: ORF TRCN0000471517

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018939.2_s317c1
Derived from:
ccsbBroadEn_15098
DNA Barcode:
AGGTACACGGCAGCGATACGACCT
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
PI4K2A (55361)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000471517
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55361 PI4K2A phosphatidylinositol 4-kina... NM_018425.3 90.8% 44.4% 164_292del;767_768insG;823_824insCG
2 mouse 84095 Pi4k2a phosphatidylinositol 4-kina... NM_145501.2 84% 42.3% (many diffs)
3 mouse 84095 Pi4k2a phosphatidylinositol 4-kina... XM_011247405.2 84% 42.3% (many diffs)
4 mouse 84095 Pi4k2a phosphatidylinositol 4-kina... XM_011247406.2 80.2% 44.2% (many diffs)
5 mouse 84095 Pi4k2a phosphatidylinositol 4-kina... XR_877696.2 52.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
732
ORF length:
663
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggacgagacg agcccactag tgtcccccga gcgggcccaa cccccggact
121acaccttccc gtcgggctcg ggcgctcact ttccgcaggt gcccgggggc gcggtccgag
181tggcggcggc ggccggctcg ggcccctctc cgccgggctc gccgggccac gaccgcgagc
241ggaacgagtt cccggaggat cctgagttcg aggcggtggt gcggcaggcc gagctggcca
301tcgagcgctg catctttccc gagcgcatct accagggctc cagcggaagc tacttcgtca
361aggaccctca ggggaggatc attgctgtct tcaaacccaa gaatgaagag ccctatgggc
421atcttaatcc taagtggacc aagtggctgc agaagctgtg ctgtccttgc tgctttggcc
481gtgactgcct tgtccttaac cagggctatc tctcagaagc aggggccagc ctggtggacc
541aaaaactgga actcaacatt gttccccgta caaaggtagt atacctggcc agtgagacct
601tcaactatag tgccattgac cgagtgaagt ccaggggcaa gcggcttgca ctagagaaag
661tgccaaaagt tggacagcgg tttaaccgca tcgggctacc accaaagggt tggttcattc
721cagctctttg ttgaaggcta caaagatgca gactattggc tgccgggcgt tttgaagcag
781aacctcttcc tgagaacact aaccggcaac tactgctcca gtttgagcgg ttggtggtgc
841tggattacat catccgcaac actgatcgag gcaatgacaa ctggctgatt aaatatgact
901gtccaatgga tagttctagc tctcgggaca cagactgggt ggtggtgaag gagcctgtta
961tcaaggtggc tgccatagac aatgggctgg ccttcccact gaagcatcct gactcctgga
1021gggcataTCC TTTTTACTGG GCCTGGTTGC CCCAGGCGAA AGTCCCATTT TCTCAGGAGA
1081TCAAAGATCT GATCCTTCCA AAGATATCGG ACCCTAACTT CGTCAAGGAC TTGGAAGAGG
1141ACCTATATGA ACTCTTCAAG AAAGATCCTG GTTTCGACAG GGGCCAGTTC CATAAGCAGA
1201TTGCTGTCAT GCGGGGCCAG ATCTTAAATC TGACCCAGGC CTTGAAAGAC AACAAGAGTC
1261CCCTGCACCT CGTCCAGATG CCACCTGTGA TTGTCGAGAC GGCCCGTTCC CACCAGCGGT
1321CTTCTAGCGA GTCCTACACA CAGAGCTTTC AGAGCCGGAA GCCCTTCTTT TCATGGTGGT
1381TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG
1441GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC
1501TTTATATATC TTGTGGAAAG GACGAAGGTA CACGGCAGCG ATACGACCTA CGCGTTAAGT
1561Cgacaatcaa cctctggatt acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)