Construct: ORF TRCN0000471517
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018939.2_s317c1
- Derived from:
- ccsbBroadEn_15098
- DNA Barcode:
- AGGTACACGGCAGCGATACGACCT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PI4K2A (55361)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471517
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55361 | PI4K2A | phosphatidylinositol 4-kina... | NM_018425.3 | 90.8% | 44.4% | 164_292del;767_768insG;823_824insCG |
| 2 | mouse | 84095 | Pi4k2a | phosphatidylinositol 4-kina... | NM_145501.2 | 84% | 42.3% | (many diffs) |
| 3 | mouse | 84095 | Pi4k2a | phosphatidylinositol 4-kina... | XM_011247405.2 | 84% | 42.3% | (many diffs) |
| 4 | mouse | 84095 | Pi4k2a | phosphatidylinositol 4-kina... | XM_011247406.2 | 80.2% | 44.2% | (many diffs) |
| 5 | mouse | 84095 | Pi4k2a | phosphatidylinositol 4-kina... | XR_877696.2 | 52.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 732
- ORF length:
- 663
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggacgagacg agcccactag tgtcccccga gcgggcccaa cccccggact 121 acaccttccc gtcgggctcg ggcgctcact ttccgcaggt gcccgggggc gcggtccgag 181 tggcggcggc ggccggctcg ggcccctctc cgccgggctc gccgggccac gaccgcgagc 241 ggaacgagtt cccggaggat cctgagttcg aggcggtggt gcggcaggcc gagctggcca 301 tcgagcgctg catctttccc gagcgcatct accagggctc cagcggaagc tacttcgtca 361 aggaccctca ggggaggatc attgctgtct tcaaacccaa gaatgaagag ccctatgggc 421 atcttaatcc taagtggacc aagtggctgc agaagctgtg ctgtccttgc tgctttggcc 481 gtgactgcct tgtccttaac cagggctatc tctcagaagc aggggccagc ctggtggacc 541 aaaaactgga actcaacatt gttccccgta caaaggtagt atacctggcc agtgagacct 601 tcaactatag tgccattgac cgagtgaagt ccaggggcaa gcggcttgca ctagagaaag 661 tgccaaaagt tggacagcgg tttaaccgca tcgggctacc accaaagggt tggttcattc 721 cagctctttg ttgaaggcta caaagatgca gactattggc tgccgggcgt tttgaagcag 781 aacctcttcc tgagaacact aaccggcaac tactgctcca gtttgagcgg ttggtggtgc 841 tggattacat catccgcaac actgatcgag gcaatgacaa ctggctgatt aaatatgact 901 gtccaatgga tagttctagc tctcgggaca cagactgggt ggtggtgaag gagcctgtta 961 tcaaggtggc tgccatagac aatgggctgg ccttcccact gaagcatcct gactcctgga 1021 gggcataTCC TTTTTACTGG GCCTGGTTGC CCCAGGCGAA AGTCCCATTT TCTCAGGAGA 1081 TCAAAGATCT GATCCTTCCA AAGATATCGG ACCCTAACTT CGTCAAGGAC TTGGAAGAGG 1141 ACCTATATGA ACTCTTCAAG AAAGATCCTG GTTTCGACAG GGGCCAGTTC CATAAGCAGA 1201 TTGCTGTCAT GCGGGGCCAG ATCTTAAATC TGACCCAGGC CTTGAAAGAC AACAAGAGTC 1261 CCCTGCACCT CGTCCAGATG CCACCTGTGA TTGTCGAGAC GGCCCGTTCC CACCAGCGGT 1321 CTTCTAGCGA GTCCTACACA CAGAGCTTTC AGAGCCGGAA GCCCTTCTTT TCATGGTGGT 1381 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1441 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1501 TTTATATATC TTGTGGAAAG GACGAAGGTA CACGGCAGCG ATACGACCTA CGCGTTAAGT 1561 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt