Construct: ORF TRCN0000471519
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018911.2_s317c1
- Derived from:
- ccsbBroadEn_15270
- DNA Barcode:
- CGCCCTGGAGCCCAAGCTTATCAA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PRAG1 (157285)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471519
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 157285 | PRAG1 | PEAK1 related, kinase-activ... | NM_001080826.3 | 91.1% | 18.4% | (many diffs) |
| 2 | human | 157285 | PRAG1 | PEAK1 related, kinase-activ... | NM_001369759.1 | 91.1% | 18.4% | (many diffs) |
| 3 | human | 157285 | PRAG1 | PEAK1 related, kinase-activ... | NR_163138.1 | 77.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1164
- ORF length:
- 1095
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcaccagacc ctctgcctga accccgagag cctgaaaatg tctgcgtgca 121 gtgactttgt ggagcacatc tggaaacccg ggtcctgcaa gaactgcttc tgcctgcgga 181 gtgaccacca gctggtggcc ggccctcccc agcccagagc gggcagcctg ccccctccac 241 cgcgcctgcc tcccaggcct gagaactgcc gcctggaaga tgaaggtgtg aacagctcac 301 cttactccaa gcccacaatt gccgtgaagc ccaccatgat gagctccgag gcctctgatg 361 tgtggacaga ggccaacctg agtgccgaag tctcgcaggt catctggaga cgagcccctg 421 gcaagctccc cctcccgaag caggaggatg cccccgtcgt ctatctgggc agcttccgag 481 gtgtacagaa gcctgctggt ccctctacct cccctgatgg caattctcgc tgtcccccag 541 cttacaccat ggtcggcctg cacaaccttg agccccgcgg cgagaggaac attgccttcc 601 acccggtgag cttcccggag gagaaggctg tgcacaaaga aaaaccctca tttccttacc 661 aagaccggcc ctccacccag gagagcttcc gccagaaact ggctgccttt gctgggacca 721 catctggctg tcaccaggcc ctgggcccct gcgggaatcc ctgccctcgg aggatgacag 781 tgatcaagtg ctcgccctcc ggggacagcg agggtggaga gtactgctca tcctggactg 841 ctgccctggg agccctgttg ccaaggctgc ctcccagact gcaggttccc ggggcaggca 901 tggtggcagg gactgctcac ccacgtgctg ggagcagggg aagtgttccg ggcccgcaga 961 gcaggagaag cggggcccga gcttccccaa ggagtgctgt agccagggcc ccactgccca 1021 cccatcctgc ctgggcccca agaaactgtc cctcacctcg gaggctgcca tttcttccga 1081 cggcctctct tgtggcagcg gcagcggcag cggcgccagt agccccttcg tcccccacct 1141 cgagagtgat tactgctccc tcatgaagga acctgcccca gagaagcagc aggaccctgg 1201 ctgcccaggg gtgaccccta gcagatgcct tgggctgacg ggggagcccc agcccccggc 1261 ccacccccgg gaggctacac agcctgaacc catctatgct gagagcacca agaggaagaa 1321 ggcagctccg gtgccttcca agtcacaggc caagatagaa catgcagctg ctgcccaggg 1381 ccaaggccag gtatgcacag gtaatgcctg ggcccagaaa gcagcatctg gctggggccg 1441 ggacagccca gacccaactc cccaggtgtc agccaccatc acagtcatgg cggcccaccc 1501 ggaagaggac catcggacga tctacctgag cagccctgac tctgcagtgg gggtgcagtg 1561 gccacgaggg cctgtgagcc agaactccga ggtaggtgaa gaggagactt cggctgggca 1621 ggggctgagc tccagggaaa gccatgctca cagtgccagc gagagcaagc ccaaggagag 1681 gcccgccatt ccccccaagt tgtccaagag tagccctgta gggtccccgg tgtcaccgtc 1741 tgctggaggg cccccagtgt caccgctggc tgaccttagt gatgggagct ctggcggcag 1801 cagcattggg ccccagcctc catcccaagg tcctgctgac cccgctcctt cctgccggac 1861 caacggtgtc gctatcagtg acccatccag gtgtccccag cctgccgcct cgtcagcctc 1921 ggaacagagg cggcccaggt tccaggcagg cacctggagt cgtcagtgcc ggatagagga 1981 agaagaggag gtggagcagg aattgctgag tcacagctgg ggaagagaga ccaaaaatgg 2041 ccccacggac cattcaaact ccacgacctg gcaccgtctc caccccacag atggctcctc 2101 tgggcagaac agcaaagttg ggaccgggat gagcaaatcc gcctcttttg cctttgagtt 2161 ccccaaggac agaagtggga ttgagacatt ctcacctcct cctccgcctc caaagtcgcg 2221 gcaccttcta aaaatgaaca agagcagctc tgatttggaa aaagtgagcc agggctctgc 2281 agaaagcctc agcccatcct tcaggggtgt ccacgtcagc ttcaccaccg gctccacgga 2341 cagcctggcc tcagactcta ggacctgcag cgatggaggt ccctcgtctg agctggctca 2401 ctcgcccacc aacagcggga agaagctctt tgctcccgtt ccgtttcctt caggctccac 2461 tgaggacgtg tcccccagtg gcccccagca gccccctcca ctcccccaga aaaagatagt 2521 gagccgggca gcctcttcac cggatggctt cttctggacc caaggctccc ccaagcccgg 2581 aacagcaagc cccaagctga acctaagcca ctcggaaacc aacgtccacg acgaatctca 2641 ctttagctat tcgttgagcc ccgggaaccg ccaccatcct gtcttctcct cttccgatcc 2701 tctggagaaa gctttcaaag gcagtggcca ctggcttccg gcagcagggc tggcgggcaa 2761 cagaggcggc tgcgggagcc ctggcctcca gtgcaaaggg gccccctccg cctcatcctc 2821 ccagctgagc gtgtccagtc aagcctccac cgggagcacc cagcttcagc tgcacggtct 2881 cctgagcaac atcagcagca aggagggcac ctatgccaag ctggggggac tctacaccca 2941 gtccctggcc cgccttgtag ccaaatgtga ggacctcttc atgggcggcc agaaaaagga 3001 gctccacttc aatgagaata actggtcgct cttcaagctg acttgtaaca agccctgctg 3061 tgactcgggg gatgccattt attactgtgc cacctgctct gaggaccccg gcagcaccta 3121 tgctgtgaaa atctgcaaag cccctgagcc caaaacagtc tcctactgca acccgttcgg 3181 gcccgtggca ttttacatcc cagcaggatt gcggccagtt ttgtggcttg ggttccgtcc 3241 agcctgtcta agttcccccg gacgggcctc cctggtcctc agcccgcccc gcctgctggt 3301 ggcactttca gccccgcaac cggccccgcc ctccccggaa gggccccgga gaaagcagct 3361 gcccggactc atcttcagca actttttgaa ggccaagcag aagccgggcg gcaccccaac 3421 cctgcagcag aagaagagcc aggcccggct ggcccccgag atcgtgtctg cttcccagta 3481 ccgcaagttc gatgagttcc agacaggcat cctcatctac gagctgctgc accaacccaa 3541 cccgttcgag gtgcgcgccc agctgcggGA GAGAGACTAC CGGCAGGAGG ACCTGCCGCC 3601 GCTGCCCGCG CTGTCCCTCT ACTCACCCGG CCTGCAGCAG CTGGCACATC TGCTACTGGA 3661 GGCCGACCCC ATCAAGCGTA TCCGCATCGG CGAGGCCAAG CGCGTGCTGC AGTGCCTGCT 3721 GTGGGGGCCT CGGCGCGAGC TGGTGCAGCA GCCGGGCACC TCGGAGGAGG CGCTGTGCGG 3781 CACGCTGCAC AACTGGATCG ACATGAAGCG GGCCCTGATG ATGATGAAGT TTGCGGAGAA 3841 GGCGGTGGAT CGCAGGCGGG GCGTGGAGCT GGAGGACTGG CTTTGCTGCC AGTACCTGGC 3901 GTCTGCGGAG CCCGGGGCCC TCTTACAGTC GCTGAAGCTC CTGCAGCTTC TGTTGCCAAC 3961 TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA 4021 TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT 4081 ATCTTGTGGA AAGGACGACG CCCTGGAGCC CAAGCTTATC AAACGCGTTA AGTCgacaat 4141 caacctctgg attacaaaat ttgtgaaaga tt