Construct: ORF TRCN0000471540
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011968.1_s317c1
- Derived from:
- ccsbBroadEn_06902
- DNA Barcode:
- CCGAACCCCTCTTCAGATAGATTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RRM1 (6240)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471540
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6240 | RRM1 | ribonucleotide reductase ca... | NM_001033.5 | 99.8% | 100% | 850C>A;2223A>G;2232G>A |
| 2 | human | 6240 | RRM1 | ribonucleotide reductase ca... | NM_001318064.1 | 87.6% | 87.7% | (many diffs) |
| 3 | human | 6240 | RRM1 | ribonucleotide reductase ca... | NM_001330193.1 | 71.8% | 71.9% | (many diffs) |
| 4 | human | 6240 | RRM1 | ribonucleotide reductase ca... | NM_001318065.1 | 57.2% | 57.3% | 0_1ins1014;1209A>G;1218G>A |
| 5 | mouse | 20133 | Rrm1 | ribonucleotide reductase M1 | NM_009103.3 | 90.1% | 97.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2442
- ORF length:
- 2376
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca tgtgatcaag cgagatggcc gccaagaacg agtcatgttt gacaaaatta 121 catctcgaat ccagaagctt tgttatggac tcaatatgga ttttgttgat cctgctcaga 181 tcaccatgaa agtaatccaa ggcttgtaca gtggggtcac cacagtggaa ctagatactt 241 tggctgctga aacagctgca accttgacta ctaagcaccc tgactatgct atcctggcag 301 ccaggatcgc tgtctctaac ttgcacaaag aaacaaagaa agtgttcagt gatgtgatgg 361 aagacctcta taactacata aatccacata atggcaaaca ctctcccatg gtggccaagt 421 caacattgga tattgttctg gccaataaag atcgcctgaa ttctgctatt atctatgacc 481 gagatttctc ttacaattac ttcggcttta agacgctaga gcggtcttat ttgttgaaga 541 tcaatggaaa agtggctgaa agaccacaac atatgttgat gagagtatct gttgggatcc 601 acaaagaaga cattgatgca gcaattgaaa catataatct tctttctgag aggtggttta 661 ctcatgcttc gcccactctc ttcaatgctg gtaccaaccg cccacaactt tctagctgtt 721 ttcttctgag tatgaaagat gacagcattg aaggcattta tgacactcta aagcaatgtg 781 cattgatttc taagtctgct ggaggaattg gtgttgctgt gagttgtatt cgggctactg 841 gcagctacat tgctgggact aatggcaatt ccaatggcct tgtaccgatg ctgagagtat 901 ataacaacac agctagatat gtggatcaag gtgggaacaa gcgtcctggg gcatttgcta 961 tttacctgga gccttggcat ttagacatct ttgaattcct tgatttaaag aagaacacag 1021 gaaaggaaga gcagcgtgcc agagatcttt tctttgctct ttggattccg gatctcttca 1081 tgaaacgagt ggagactaat caggactggt ctttgatgtg tccaaatgag tgtcctggtc 1141 tggatgaggt ttggggagag gaatttgaga aactatatgc aagttatgag aaacaaggtc 1201 gtgtccgcaa agttgtaaaa gctcagcagc tttggtatgc catcattgag tctcagacgg 1261 aaacaggcac cccgtatatg ctctacaaag attcctgtaa tcgaaagagc aaccagcaga 1321 acctgggaac catcaaatgc agcaacctgt gcacagaaat agtggagtac accagcaaag 1381 atgaggttgc tgtttgtaat ttggcttccc tggccctgaa tatgtatgtc acatcagaac 1441 acacatacga ctttaagaag ttggctgaag tcactaaagt cgttgtccga aacttgaata 1501 aaattattga tataaactac tatcctgtac cagaggcatg cctatcaaat aaacgccatc 1561 gccccattgg aattggggta caaggtctgg cagatgcttt tatcctgatg agataccctt 1621 ttgagagtgc agaagcccag ttactgaata agcagatctt tgaaactatt tattatggtg 1681 ctctggaagc cagctgtgac cttgccaagg agcagggccc atacgaaacc tatgagggct 1741 ctccagttag caaaggaatt cttcagtatg atatgtggaa tgttactcct acagacctat 1801 gggactggaa ggttctcaag gagaagattg caaagtatgg tataagaaac agtttactta 1861 ttgccccgat gcctacagct tccactgctc agatcctggg gaataatgag tccattgaac 1921 cttacaccag caacatctat actcgcagag tcttgtcagg agaatttcag attgtaaatc 1981 ctcacttatt gaaagatctt accgagcggg gccTATGGCA TGAAGAGATG AAAAACCAGA 2041 TTATTGCATG CAATGGCTCT ATTCAGAGCA TACCAGAAAT TCCTGATGAC CTGAAGCAAC 2101 TTTATAAAAC TGTGTGGGAA ATCTCTCAGA AAACTGTTCT CAAGATGGCA GCTGAGAGAG 2161 GTGCTTTCAT TGATCAAAGC CAATCTTTGA ACATCCACAT TGCTGAGCCT AACTATGGCA 2221 AACTCACTAG TATGCACTTC TACGGCTGGA AGCAGGGTTT GAAGACTGGG ATGTATTATT 2281 TAAGGACGAG ACCAGCAGCT AATCCAATCC AGTTCACTCT AAATAAGGAG AAGCTAAAAG 2341 ATAAAGAAAA GGTATCAAAA GAGGAAGAAG AGAAGGAGAG GAACACAGCA GCCATGGTGT 2401 GCTCTTTGGA GAATAGAGAT GAATGTCTGA TGTGTGGATC CTGCCCAACT TTCTTGTACA 2461 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 2521 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 2581 AGGACGACCG AACCCCTCTT CAGATAGATT TACGCGTTAA GTCgacaatc aacctctgga 2641 ttacaaaatt tgtgaaagat t