Construct: ORF TRCN0000471571
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018538.1_s317c1
- Derived from:
- ccsbBroadEn_00294
- DNA Barcode:
- GCCGCTCGCTTACGCTACTTCGTA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- CETN3 (1070)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471571
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1070 | CETN3 | centrin 3 | NM_004365.3 | 100% | 100% | |
| 2 | human | 1070 | CETN3 | centrin 3 | NM_001297765.1 | 87.4% | 87.4% | 461_532del |
| 3 | human | 1070 | CETN3 | centrin 3 | NM_001297768.1 | 85.1% | 80% | (many diffs) |
| 4 | mouse | 12626 | Cetn3 | centrin 3 | NM_007684.3 | 89% | 98.8% | (many diffs) |
| 5 | mouse | 12626 | Cetn3 | centrin 3 | XM_006517074.3 | 76.5% | 83% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 567
- ORF length:
- 501
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag tttagctctg agaagtgagc ttgtagtgga caaaacaaag aggaaaaaaa 121 gaagagaact gtctgaggaa cagaaaCAAG AAATTAAAGA TGCTTTTGAA CTATTTGATA 181 CAGACAAAGA TGAAGCAATA GATTATCATG AATTAAAGGT GGCAATGAGA GCCTTGGGGT 241 TTGATGTAAA AAAAGCTGAT GTACTGAAGA TTCTTAAAGA TTATGACAGA GAAGCCACAG 301 GGAAAATCAC CTTTGAAGAT TTTAATGAAG TTGTGACAGA CTGGATATTG GAAAGAGATC 361 CCCATGAAGA AATACTCAAG GCATTTAAAC TATTTGATGA TGATGATTCA GGTAAAATAA 421 GCTTGAGGAA TTTGCGACGT GTTGCTAGAG AATTGGGTGA AAACATGAGT GATGAAGAAC 481 TTCGAGCTAT GATAGAAGAA TTTGACAAAG ATGGTGATGG AGAAATAAAC CAAGAGGAGT 541 TCATTGCTAT TATGACTGGT GACATTTAAC CAACTTTCTT GTACAAAGTG GTTGATATCG 601 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 661 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGCCGCTCG 721 CTTACGCTAC TTCGTAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 781 aagatt