Construct: ORF TRCN0000471572
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015235.1_s317c1
- Derived from:
- ccsbBroadEn_07775
- DNA Barcode:
- CAGGTTTGCGTCCCTCCTCAGACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPL35 (11224)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471572
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11224 | RPL35 | ribosomal protein L35 | NM_007209.4 | 99.7% | 99.1% | 365A>G |
| 2 | mouse | 66489 | Rpl35 | ribosomal protein L35 | NM_025592.3 | 88.8% | 97.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 435
- ORF length:
- 369
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttGGCATGGC CAAGATCAAG GCTCGAGATC TTCGCGGGAA GAAGAAGGAG GAGCTGCTGA 121 AACAGCTGGA CGACCTGAAG GTGGAGCTGT CCCAGCTGCG CGTCGCCAAA GTGACAGGCG 181 GTGCGGCCTC CAAGCTCTCT AAGATCCGAG TCGTCCGGAA ATCCATTGCC CGTGTTCTCA 241 CAGTTATTAA CCAGACTCAG AAAGAAAACC TCAGGAAATT CTACAAGGGC AAGAAGTACA 301 AGCCCCTGGA CCTGCGGCCT AAGAAGACAC GTGCCATGCG CCGCCGGCTC AACAAGCACG 361 AGGAGAACCT GAAGACCAAG AAGCAGCAGC GGAAGGAGCG GCTGTACCCG CTGCGGAAGT 421 ACGCGGTCAG GGCCTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 481 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 541 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CAGGTTTGCG TCCCTCCTCA 601 GACAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt