Construct: ORF TRCN0000471573
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010694.1_s317c1
- Derived from:
- ccsbBroadEn_13859
- DNA Barcode:
- TCGTCGCGCTCTGCGGGCTGATTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FUT2 (2524)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471573
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2524 | FUT2 | fucosyltransferase 2 | NM_000511.6 | 44.2% | 44% | (many diffs) |
| 2 | human | 2524 | FUT2 | fucosyltransferase 2 | NM_001097638.3 | 44.2% | 44% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 525
- ORF length:
- 459
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct ggtcgttcag atgcctttct cctttcccat ggcccacttc atcctctttg 121 tctttacggt ttcCACTATA TTTCACGTTC AGCAGCGGCT AGCGAAGATT CAAGCCATGT 181 GGGAGTTACC GGTGCAGATA CCAGTGCTAG CCTCAACATC AAAGGCACTG GGACCCAGCC 241 AGCTCAGGGG GATGTGGACG ATCAATGCGA TAGGCCGCCT GGGGAACCAG ATGGGCGAGT 301 ACGCCACACT GTATGCCCTG GCCAAGATGA ACGGGCGGCC CGCCTTCATC CCGGCCCAGA 361 TGCACAGCAC CCTGGCCCCC ATCTTCAGAA TCACCCTGCC GGTGCTGCAC AGCGCCACGG 421 CCAGCAGGAT CCCCTGGCAG AACTACCACC TGAACGACTG GATGGAGGAG GAATACCGCC 481 ACATCCCGGG GGAGTACGTC CGCTTCACCG GCTACCGCAG CTCCTACCCG ACTTTCTTGT 541 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 601 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 661 GAAAGGACGA TCGTCGCGCT CTGCGGGCTG ATTAACGCGT TAAGTCgaca atcaacctct 721 ggattacaaa atttgtgaaa gatt