Construct: ORF TRCN0000471578
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015285.1_s317c1
- Derived from:
- ccsbBroadEn_02909
- DNA Barcode:
- AAGCTCCCGCCGAATCAGTCTAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HACL1 (26061)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471578
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26061 | HACL1 | 2-hydroxyacyl-CoA lyase 1 | NM_012260.4 | 100% | 100% | |
| 2 | human | 26061 | HACL1 | 2-hydroxyacyl-CoA lyase 1 | NM_001284413.2 | 95.3% | 95.3% | 226_227ins81 |
| 3 | human | 26061 | HACL1 | 2-hydroxyacyl-CoA lyase 1 | NM_001284415.2 | 89.6% | 89.6% | 379_380ins78;913_914ins102 |
| 4 | human | 26061 | HACL1 | 2-hydroxyacyl-CoA lyase 1 | NM_001284416.2 | 85.8% | 85.8% | 308_309ins246 |
| 5 | human | 26061 | HACL1 | 2-hydroxyacyl-CoA lyase 1 | NR_104315.2 | 77.4% | (many diffs) | |
| 6 | mouse | 56794 | Hacl1 | 2-hydroxyacyl-CoA lyase 1 | NM_019975.3 | 85.5% | 87.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1800
- ORF length:
- 1734
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc ggacagtaac ttcgcagagc gcagcgagga gcaggtgtct ggtgctaaag 121 tcatcgctca ggccctgaaa acgcaagatg tggagtacat atttggcatc gtaggcatcc 181 cagtgaccga aatcgccatt gctgcccagc agctaggcat caagtacatc gggatgagga 241 atgagcaagc ggcttgttat gctgcctccg cgattggata tctgacaagc aggccaggag 301 tctgccttgt tgtttctggc ccaggtctca tccatgcctt gggcggtatg gcaaatgcaa 361 acatgaactg ctggcccttg cttgtgattg gtggttcctc tgaaagaaac caagaaacaa 421 tgggagcttt ccaggagttt cctcaggttg aagcttgtag attatatacc aagttctctg 481 cccgcccaag cagcatagaa gctattcctt ttgttattga aaaggcagtg agaagcagta 541 tctatggtcg tccaggtgct tgctatgttg acataccagc agattttgtg aaccttcagg 601 tgaatgtgaa ttctataaag tacatggaac gctgcatgtc acctcctatt agcatggcag 661 aaacctctgc tgtgtgcacg gcggcttctg ttattaggaa tgccaaacaa ccccttctta 721 tcatcgggaa aggtgctgct tacgctcatg cagaagagag tatcaagaaa ttggtggagc 781 aatataaact gccatttttg cccaccccta tggggaaggg tgttgtccct gacaaccatc 841 catactgtgt aggtgcagcc agatccaggg ctttgcaatt tgctgatgta attgtgttat 901 ttggtgccag actaaattgg attttacatt ttggactgcc tccaagatat cagccagatg 961 tgaagtttat ccaggttgat atctgtgcag aagaattggg gaataatgta aagcccgctg 1021 ttactttgct aggaaacata catgctgtca ctaagcagct tttagaggaa cttgataaaa 1081 caccatggca gtatcctcca gagagcaagt ggtggaaaac tctgagagaa aaaatgaaga 1141 gcaatgaagc tgcatccaag gaactagctt ctaaaaaatc cctgcctatg aattattaca 1201 cagtattcta ccatgttcaa gaacaactac ctagagactg tttcgtggta agtgaaggag 1261 caaatactat ggacattgga cggactgtgc ttcagaacta ccttcctcgt cacaggcttg 1321 atgctggtac tttcggaaca atgggagttg gtttgggatt tgctattgca gctgccgtgg 1381 tggctaaaga tagaagccct gggcaatgga tcatctgtgt ggaaggagac agtgcatttg 1441 ggttttctgg catggaggta gaaaccatct gcaggtacaa cttgccaaTC ATACTGTTGG 1501 TAGTGAATAA CAATGGAATT TACCAAGGTT TTGATACAGA TACTTGGAAA GAAATGTTAA 1561 AATTTCAAGA TGCTACTGCA GTGGTCCCTC CAATGTGTTT GCTGCCAAAT TCACATTATG 1621 AGCAAGTCAT GACTGCATTT GGAGGCAAAG GGTATTTTGT ACAAACACCA GAAGAACTCC 1681 AAAAATCCCT GAGGCAGAGC CTAGCAGACA CAACTAAACC TTCTCTTATC AACATCATGA 1741 TTGAGCCACA AGCCACACGG AAGGCCCAGG ATTTTCATTG GCTGACCCGC TCTAATATGT 1801 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1861 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1921 TTTATATATC TTGTGGAAAG GACGAAAGCT CCCGCCGAAT CAGTCTAAGA CGCGTTAAGT 1981 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt