Construct: ORF TRCN0000471607
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009913.1_s317c1
- Derived from:
- ccsbBroadEn_11023
- DNA Barcode:
- GTCAGCGCGAATCGTGATTTCGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PDE4D (5144)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471607
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5144 | PDE4D | phosphodiesterase 4D | NM_001364602.2 | 100% | 100% | |
| 2 | human | 5144 | PDE4D | phosphodiesterase 4D | NM_001364600.2 | 74.2% | 61% | (many diffs) |
| 3 | human | 5144 | PDE4D | phosphodiesterase 4D | NM_001364601.1 | 69.2% | 68.9% | 456_456delGins202 |
| 4 | human | 5144 | PDE4D | phosphodiesterase 4D | NM_001197218.2 | 28.9% | 27.9% | (many diffs) |
| 5 | human | 5144 | PDE4D | phosphodiesterase 4D | XM_017009567.1 | 23.2% | 18.1% | (many diffs) |
| 6 | human | 5144 | PDE4D | phosphodiesterase 4D | NM_001349241.2 | 23.2% | 18.2% | (many diffs) |
| 7 | human | 5144 | PDE4D | phosphodiesterase 4D | NM_001165899.2 | 23.2% | 17.9% | (many diffs) |
| 8 | human | 5144 | PDE4D | phosphodiesterase 4D | NM_001364599.1 | 23.2% | 17.9% | (many diffs) |
| 9 | human | 5144 | PDE4D | phosphodiesterase 4D | XM_011543471.2 | 23.2% | 17.9% | (many diffs) |
| 10 | human | 5144 | PDE4D | phosphodiesterase 4D | XM_011543473.1 | 23.2% | 17.9% | (many diffs) |
| 11 | human | 5144 | PDE4D | phosphodiesterase 4D | XM_017009566.1 | 23.2% | 17.9% | (many diffs) |
| 12 | human | 5144 | PDE4D | phosphodiesterase 4D | XM_024446112.1 | 23.2% | 17.9% | (many diffs) |
| 13 | human | 5144 | PDE4D | phosphodiesterase 4D | XM_011543469.1 | 23.1% | 17.8% | (many diffs) |
| 14 | human | 5144 | PDE4D | phosphodiesterase 4D | XM_011543470.2 | 23.1% | 17.8% | (many diffs) |
| 15 | human | 5144 | PDE4D | phosphodiesterase 4D | XM_017009565.1 | 23.1% | 17.8% | (many diffs) |
| 16 | human | 5144 | PDE4D | phosphodiesterase 4D | XM_024446110.1 | 23.1% | 17.8% | (many diffs) |
| 17 | human | 5144 | PDE4D | phosphodiesterase 4D | NM_001349242.1 | 20.2% | 17.8% | (many diffs) |
| 18 | human | 5144 | PDE4D | phosphodiesterase 4D | NM_001197219.2 | 20% | 16.7% | (many diffs) |
| 19 | human | 5144 | PDE4D | phosphodiesterase 4D | NM_001197220.2 | 19.5% | 17.1% | (many diffs) |
| 20 | human | 5144 | PDE4D | phosphodiesterase 4D | NM_006203.4 | 18.4% | 16.7% | (many diffs) |
| 21 | mouse | 238871 | Pde4d | phosphodiesterase 4D, cAMP ... | XM_006517644.2 | 26.6% | 27.7% | (many diffs) |
| 22 | mouse | 238871 | Pde4d | phosphodiesterase 4D, cAMP ... | XM_006517646.3 | 18.7% | 17.2% | (many diffs) |
| 23 | mouse | 238871 | Pde4d | phosphodiesterase 4D, cAMP ... | XM_006517647.2 | 18.4% | 17.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 723
- ORF length:
- 657
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tcagcagaca agcccggaca ctttaacagt acctgaagtg gataatccgc 121 attgtccaaa cccgtggctg aacgaagacc ttgtgaaatc cttgcgagaa aacctgttgc 181 agcatgagaa gtccAAGACA GCGAGGAAAT CGGTTTCTCC CAAGCTCTCT CCAGTGATCT 241 CTCCGAGAAA TTCCCCCAGG CTTCTGCGCA GAATGCTTCT CAGCAGCAAC ATCCCCAAAC 301 AGCGGCGTTT CACGGTGGCA CATACATGTT TTGATGTGGA CAATGGCACA TCTGCGGGAC 361 GGAGTCCCTT GGATCCCATG ACCAGCCCAG GATCCGGGCT AATTCTCCAA GCAAATTTTG 421 TCCACAGTCA ACGACGGGAG TCCTTCCTGT ATCGATCCGA CAGCGATTAT GACCTCTCTC 481 CAAAGTCTAT GTCCCGGAAC TCCTCCATTG CCAGTGATAT ACACGGAGAT GACTTGATTG 541 TGACTCCATT TGCTCAGGTC TTGGCCAGTC TGCGAACTGT ACGAAACAAC TTTGCTGCAT 601 TAACTAATTT GCAAGATCGA GCACCTAGCA AAAGATCACC CATGTGCAAC CAACCATCCA 661 TCAACAAAGC CACCATAACA GGATCCTGGA TGGAATTGAA CCCATATACG TTACTTGACA 721 TGTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 781 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 841 GGCTTTATAT ATCTTGTGGA AAGGACGAGT CAGCGCGAAT CGTGATTTCG TTACGCGTTA 901 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt