Construct: ORF TRCN0000471612
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004733.1_s317c1
- Derived from:
- ccsbBroadEn_09511
- DNA Barcode:
- TCTGGAAACGCGAGTCAAAATAGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IQGAP3 (128239)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471612
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 128239 | IQGAP3 | IQ motif containing GTPase ... | NM_178229.5 | 99.8% | 99.8% | (many diffs) |
| 2 | human | 128239 | IQGAP3 | IQ motif containing GTPase ... | XM_011509198.3 | 99.5% | 99.4% | (many diffs) |
| 3 | human | 128239 | IQGAP3 | IQ motif containing GTPase ... | XM_024453275.1 | 99.4% | 99.3% | (many diffs) |
| 4 | human | 128239 | IQGAP3 | IQ motif containing GTPase ... | XM_024453273.1 | 99.1% | 99% | (many diffs) |
| 5 | human | 128239 | IQGAP3 | IQ motif containing GTPase ... | XM_024453274.1 | 99% | 98.9% | (many diffs) |
| 6 | human | 128239 | IQGAP3 | IQ motif containing GTPase ... | XM_024453276.1 | 91.2% | 91.2% | (many diffs) |
| 7 | human | 128239 | IQGAP3 | IQ motif containing GTPase ... | XM_011509200.2 | 72.8% | 72.8% | 0_1ins1326;661C>T;3006A>G |
| 8 | human | 128239 | IQGAP3 | IQ motif containing GTPase ... | XM_011509201.2 | 69.5% | 69.5% | 0_1ins1488;499C>T;2844A>G |
| 9 | mouse | 404710 | Iqgap3 | IQ motif containing GTPase ... | NM_001033484.1 | 85.1% | 85.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 4962
- ORF length:
- 4893
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagaggaga gcagcgggcc caggctgggc agcctatgaa cgcctcacag 121 ctgaggagat ggatgagcag aggcggcaga atgttgccta tcagtacctg tgccggctgg 181 aggaggccaa gcgctggatg gaggcctgcc tgaaggagga gcttccttcc ccggtggagc 241 tggaggagag ccttcggaat ggagtgctgc tggccaagct gggccactgt tttgcaccct 301 ccgtggttcc gttgaagaag atctacgatg tggagcagct gcggtaccag gcaactggct 361 tacatttccg tcacacagac aacatcaact tttggctatc tgcaatagcc cacatcggtc 421 tgccttcgac cttcttccca gagaccacgg acatctatga caaaaagaac atgccccggg 481 tagtctactg catccatgct ctcagtctct tcctcttccg gctgggattg gcccctcaga 541 tacatgatct atacgggaaa gtgaaattca cagctgagga actcagcaac atggcgtccg 601 aactggccaa atatggcctc cagctgcctg ccttcagcaa gatcgggggc atcttggcca 661 atgagctctc ggtggatgag gctgcagtcc atgcagctgt tcttgccatc aatgaagcag 721 tggagcgagg ggtggtggag gacaccctgg ctgccttgca gaatcccagt gctcttctgg 781 agaatctccg agagcctctg gcagccgtct accaggagat gctggcccag gccaagatgg 841 agaaggcagc caatgccagg aaccatgatg acagagaaag ccaggacatc tatgaccact 901 acctaactca ggctgaaatc cagggcaata tcaaccatgt caacgcccat ggggctctag 961 aagttgttga tgatgccctg gaaagacaga gccctgaagc cttgctcaag gcccttcaag 1021 accctgccct ggccctgcga ggggtgagga gagactttgc tgactggtac ctggagcagc 1081 tgaactcaga cagagagcag aaggcacagg agctgggcct ggtggagctt ctggaaaagg 1141 aggaagtcca ggctggtgtg gctgcagcca acacaaaggg tgatcaggaa caagccatgc 1201 tccacgctgt gcagcggatc aacaaagcca tccggagggg agtggcggct gacactgtga 1261 aggagctgat gtgccctgag gcccagctgc ctccagtgta ccctgttgca tcgtctatgt 1321 accagctgga gctggcagtg ctccagcagc agcaggggga gcttggccag gaggagctct 1381 tcgtggctgt ggagatgctc tcagctgtgg tcctgattaa ccgggccctg gaggcccggg 1441 atgccagtgg cttctggagc agcctggtga accctgccac aggcctggct gaggtggaag 1501 gagaaaatgc ccagcgttac ttcgatgccc tgctgaaatt gcgacaggag cgtgggatgg 1561 gtgaggactt cctgagctgg aatgacctgc aggccaccgt gagccaggtc aatgcacaga 1621 cccaggaaga gactgaccgg gtccttgcag tcagcctcat caatgaggct ctggacaaag 1681 gcagccctga gaagactctg tctgccctac tgcttcctgc agctggccta gatgatgtca 1741 gcctccctgt cgcccctcgg taccatctcc tccttgtggc agccaaaagg cagaaggccc 1801 aggtgacagg ggatcctgga gctgtgctgt ggcttgagga gatccgccag ggagtggtca 1861 gagccaacca ggacactaat acagctcaga gaatggctct tggtgtggct gccatcaatc 1921 aagccatcaa ggagggcaag gcagcccaga ctgagcgggt gttgaggaac cccgcagtgg 1981 cccttcgagg ggtagttccc gactgtgcca acggctacca gcgagccctg gaaagtgcca 2041 tggcaaagaa acagtgtcca gcagacacag ctttctgggt tcaacatgac atgaaggatg 2101 gcactgccta ctacttccat ctgcagacct tccaggggat ctgggagcaa cctcctggct 2161 gccccctcaa cacctctcac ctgacccggg aggagatcca gtcagctgtc accaaggtca 2221 ctgctgccta tgaccgccaa cagctctgga aagccaacgt cggctttgtt atccagctcc 2281 aggcccgcct ccgtggcttc ctagttcggc agaagtttgc tgagcattcc cactttctga 2341 ggacctggct cccagcagtc atcaagatcc aggctcattg gcggggttat aggcagcgga 2401 agatttacct ggagtggttg cagtatttta aagcaaacct ggatgccata atcaagatcc 2461 aggcctgggc ccggatgtgg gcagctcgga ggcaatacct gaggcgtctg cactacttcc 2521 agaagaatgt taactccatt gtgaagatcc aggcattttt ccgagccagg aaagcccaag 2581 atgactacag gatattagtg catgcacccc accctcctct cagtgtggta cgcagatttg 2641 cccatctctt gaatcaaagc cagcaagact tcttggctga ggcagagctg ctgaagctcc 2701 aggaagaggt agttaggaag atccgatcca atcagcagct ggagcaggac ctcaacatca 2761 tggacatcaa gattggcctg ctggtgaaga accggatcac tctgcaggaa gtggtctccc 2821 actgcaagaa gctgaccaag aggaataagg aacagctgtc agatatgatg gttctggaca 2881 agcagaaggg tttaaagtcg ctgagcaaag agaaacggca gaaactagaa gcataccaac 2941 acctcttcta cctgctccag actcagccca tctacctggc caagctgatc tttcagatgc 3001 cacagaacaa aaccaccaag ttcatggagg cagtgatttt cagcctgtac aactatgcct 3061 ccagccgccg agaggcctat ctcctgctcc agctgttcaa gacagcactc caggaggaaa 3121 tcaagtcaaa ggtggagcag ccccaggacg tggtgacagg caacccaaca gtggtgaggc 3181 tggtggtgag attctaccgt aatgggcggg gacagagtgc cctgcaggag attctgggca 3241 aggttatcca ggatgtgcta gaagacaaag tgctcagcgt ccacacagac cctgtccacc 3301 tctataagaa ctggatcaac cagactgagg cccagacagg gcagcgcagc catctcccat 3361 atgatgtcac cccggagcag gccttgagcc accccgaggt ccagagacga ctggacatcg 3421 ccctacgcaa cctcctcgcc atgactgata agttcctttt agccatcacc tcatctgtgg 3481 accaaattcc gtatgggatg cgatatgtgg ccaaagtcct gaaggcaact ctggcagaga 3541 aattccctga cgccacagac agcgaggtct ataaggtggt cgggaacctc ctgtactacc 3601 gcttcctgaa cccagctgtg gtggctcctg acgccttcga cattgtggcc atggcagctg 3661 gtggagccct ggctgccccc cagcgccatg ccctgggggc tgtggctcag ctcctacagc 3721 acgctgcggc tggcaaggcc ttctctgggc agagccagca cctacgggtc ctgaatgact 3781 atctggagga aacacacctc aagttcagga agttcatcca tagagcctgc caggtgccag 3841 agccagagga gcgttttgca gtggacgagt actcagacat ggtggctgtg gccaaaccca 3901 tggtgtacat caccgtgggg gagctggtca acacgcacag gctgttgctg gagcaccagg 3961 actgcattgc ccctgatcac caagaccccc tgcatgagct cctggaggat cttggggagc 4021 tgcccaccat ccctgacctt attggtgaga gcatcgctgc agatgggcac acggacctga 4081 gcaagctaga agtgtccctg acgctgacca acaagtttga aggactagag gcagatgctg 4141 atgactccaa cacccgtagc ctgcttctga gcaccaagca gctgttggcc gatatcatac 4201 agttccatcc tggggacacc ctcaaggaga tcctgtccct ctcggcttcc agagagcaag 4261 aagcagccca caagcagctg atgagccgac gccaggcctg tacagcccag acaccggagc 4321 cactgcgacg acaccgctca ctgacagctc actccctcct gccactggca gagaagcagc 4381 ggcgcgtcct gcggaacctg cgccgacttg aagccctggg gttggtcagc gccagaaatg 4441 gctaccaggg gctagtggac gagctggcca aggacatccg caaccagcac agacacaggc 4501 acaggcggaa ggcagagctg gtgaagctgc aggccacatt acagggcctg agcactaaga 4561 ccaccttcta tgaggagcag ggtgactact acagccagta catccgggcc tgcctggacc 4621 acctggcccc cgactccaag agttctggga aggggaagaa gcagccttct cttcattaca 4681 ctgctgctca gcTCCTGGAA AAGGGTGTCT TGGTGGAAAT TGAAGATCTT CCCGCCTCTC 4741 ACTTCAGAAA CGTCATCTTT GACATCACGC CGGGAGATGA GGCAGGAAAG TTTGAAGTAA 4801 ATGCCAAGTT CCTGGGTGTG GACATGGAGC GATTTCAGCT TCACTATCAG GATCTCCTGC 4861 AGCTCCAGTA TGAGGGTGTG GCTGTCATGA AACTCTTCAA CAAGGCCAAA GTCAATGTCA 4921 ACCTTCTCAT CTTCCTCCTC AACAAGAAGT TTTTGCGGAA GTTGCCAACT TTCTTGTACA 4981 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 5041 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 5101 AGGACGATCT GGAAACGCGA GTCAAAATAG GACGCGTTAA GTCgacaatc aacctctgga 5161 ttacaaaatt tgtgaaagat t