Construct: ORF TRCN0000471612

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004733.1_s317c1
Derived from:
ccsbBroadEn_09511
DNA Barcode:
TCTGGAAACGCGAGTCAAAATAGG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
IQGAP3 (128239)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000471612
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 128239 IQGAP3 IQ motif containing GTPase ... NM_178229.5 99.8% 99.8% (many diffs)
2 human 128239 IQGAP3 IQ motif containing GTPase ... XM_011509198.3 99.5% 99.4% (many diffs)
3 human 128239 IQGAP3 IQ motif containing GTPase ... XM_024453275.1 99.4% 99.3% (many diffs)
4 human 128239 IQGAP3 IQ motif containing GTPase ... XM_024453273.1 99.1% 99% (many diffs)
5 human 128239 IQGAP3 IQ motif containing GTPase ... XM_024453274.1 99% 98.9% (many diffs)
6 human 128239 IQGAP3 IQ motif containing GTPase ... XM_024453276.1 91.2% 91.2% (many diffs)
7 human 128239 IQGAP3 IQ motif containing GTPase ... XM_011509200.2 72.8% 72.8% 0_1ins1326;661C>T;3006A>G
8 human 128239 IQGAP3 IQ motif containing GTPase ... XM_011509201.2 69.5% 69.5% 0_1ins1488;499C>T;2844A>G
9 mouse 404710 Iqgap3 IQ motif containing GTPase ... NM_001033484.1 85.1% 85.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
4962
ORF length:
4893
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggagaggaga gcagcgggcc caggctgggc agcctatgaa cgcctcacag
121ctgaggagat ggatgagcag aggcggcaga atgttgccta tcagtacctg tgccggctgg
181aggaggccaa gcgctggatg gaggcctgcc tgaaggagga gcttccttcc ccggtggagc
241tggaggagag ccttcggaat ggagtgctgc tggccaagct gggccactgt tttgcaccct
301ccgtggttcc gttgaagaag atctacgatg tggagcagct gcggtaccag gcaactggct
361tacatttccg tcacacagac aacatcaact tttggctatc tgcaatagcc cacatcggtc
421tgccttcgac cttcttccca gagaccacgg acatctatga caaaaagaac atgccccggg
481tagtctactg catccatgct ctcagtctct tcctcttccg gctgggattg gcccctcaga
541tacatgatct atacgggaaa gtgaaattca cagctgagga actcagcaac atggcgtccg
601aactggccaa atatggcctc cagctgcctg ccttcagcaa gatcgggggc atcttggcca
661atgagctctc ggtggatgag gctgcagtcc atgcagctgt tcttgccatc aatgaagcag
721tggagcgagg ggtggtggag gacaccctgg ctgccttgca gaatcccagt gctcttctgg
781agaatctccg agagcctctg gcagccgtct accaggagat gctggcccag gccaagatgg
841agaaggcagc caatgccagg aaccatgatg acagagaaag ccaggacatc tatgaccact
901acctaactca ggctgaaatc cagggcaata tcaaccatgt caacgcccat ggggctctag
961aagttgttga tgatgccctg gaaagacaga gccctgaagc cttgctcaag gcccttcaag
1021accctgccct ggccctgcga ggggtgagga gagactttgc tgactggtac ctggagcagc
1081tgaactcaga cagagagcag aaggcacagg agctgggcct ggtggagctt ctggaaaagg
1141aggaagtcca ggctggtgtg gctgcagcca acacaaaggg tgatcaggaa caagccatgc
1201tccacgctgt gcagcggatc aacaaagcca tccggagggg agtggcggct gacactgtga
1261aggagctgat gtgccctgag gcccagctgc ctccagtgta ccctgttgca tcgtctatgt
1321accagctgga gctggcagtg ctccagcagc agcaggggga gcttggccag gaggagctct
1381tcgtggctgt ggagatgctc tcagctgtgg tcctgattaa ccgggccctg gaggcccggg
1441atgccagtgg cttctggagc agcctggtga accctgccac aggcctggct gaggtggaag
1501gagaaaatgc ccagcgttac ttcgatgccc tgctgaaatt gcgacaggag cgtgggatgg
1561gtgaggactt cctgagctgg aatgacctgc aggccaccgt gagccaggtc aatgcacaga
1621cccaggaaga gactgaccgg gtccttgcag tcagcctcat caatgaggct ctggacaaag
1681gcagccctga gaagactctg tctgccctac tgcttcctgc agctggccta gatgatgtca
1741gcctccctgt cgcccctcgg taccatctcc tccttgtggc agccaaaagg cagaaggccc
1801aggtgacagg ggatcctgga gctgtgctgt ggcttgagga gatccgccag ggagtggtca
1861gagccaacca ggacactaat acagctcaga gaatggctct tggtgtggct gccatcaatc
1921aagccatcaa ggagggcaag gcagcccaga ctgagcgggt gttgaggaac cccgcagtgg
1981cccttcgagg ggtagttccc gactgtgcca acggctacca gcgagccctg gaaagtgcca
2041tggcaaagaa acagtgtcca gcagacacag ctttctgggt tcaacatgac atgaaggatg
2101gcactgccta ctacttccat ctgcagacct tccaggggat ctgggagcaa cctcctggct
2161gccccctcaa cacctctcac ctgacccggg aggagatcca gtcagctgtc accaaggtca
2221ctgctgccta tgaccgccaa cagctctgga aagccaacgt cggctttgtt atccagctcc
2281aggcccgcct ccgtggcttc ctagttcggc agaagtttgc tgagcattcc cactttctga
2341ggacctggct cccagcagtc atcaagatcc aggctcattg gcggggttat aggcagcgga
2401agatttacct ggagtggttg cagtatttta aagcaaacct ggatgccata atcaagatcc
2461aggcctgggc ccggatgtgg gcagctcgga ggcaatacct gaggcgtctg cactacttcc
2521agaagaatgt taactccatt gtgaagatcc aggcattttt ccgagccagg aaagcccaag
2581atgactacag gatattagtg catgcacccc accctcctct cagtgtggta cgcagatttg
2641cccatctctt gaatcaaagc cagcaagact tcttggctga ggcagagctg ctgaagctcc
2701aggaagaggt agttaggaag atccgatcca atcagcagct ggagcaggac ctcaacatca
2761tggacatcaa gattggcctg ctggtgaaga accggatcac tctgcaggaa gtggtctccc
2821actgcaagaa gctgaccaag aggaataagg aacagctgtc agatatgatg gttctggaca
2881agcagaaggg tttaaagtcg ctgagcaaag agaaacggca gaaactagaa gcataccaac
2941acctcttcta cctgctccag actcagccca tctacctggc caagctgatc tttcagatgc
3001cacagaacaa aaccaccaag ttcatggagg cagtgatttt cagcctgtac aactatgcct
3061ccagccgccg agaggcctat ctcctgctcc agctgttcaa gacagcactc caggaggaaa
3121tcaagtcaaa ggtggagcag ccccaggacg tggtgacagg caacccaaca gtggtgaggc
3181tggtggtgag attctaccgt aatgggcggg gacagagtgc cctgcaggag attctgggca
3241aggttatcca ggatgtgcta gaagacaaag tgctcagcgt ccacacagac cctgtccacc
3301tctataagaa ctggatcaac cagactgagg cccagacagg gcagcgcagc catctcccat
3361atgatgtcac cccggagcag gccttgagcc accccgaggt ccagagacga ctggacatcg
3421ccctacgcaa cctcctcgcc atgactgata agttcctttt agccatcacc tcatctgtgg
3481accaaattcc gtatgggatg cgatatgtgg ccaaagtcct gaaggcaact ctggcagaga
3541aattccctga cgccacagac agcgaggtct ataaggtggt cgggaacctc ctgtactacc
3601gcttcctgaa cccagctgtg gtggctcctg acgccttcga cattgtggcc atggcagctg
3661gtggagccct ggctgccccc cagcgccatg ccctgggggc tgtggctcag ctcctacagc
3721acgctgcggc tggcaaggcc ttctctgggc agagccagca cctacgggtc ctgaatgact
3781atctggagga aacacacctc aagttcagga agttcatcca tagagcctgc caggtgccag
3841agccagagga gcgttttgca gtggacgagt actcagacat ggtggctgtg gccaaaccca
3901tggtgtacat caccgtgggg gagctggtca acacgcacag gctgttgctg gagcaccagg
3961actgcattgc ccctgatcac caagaccccc tgcatgagct cctggaggat cttggggagc
4021tgcccaccat ccctgacctt attggtgaga gcatcgctgc agatgggcac acggacctga
4081gcaagctaga agtgtccctg acgctgacca acaagtttga aggactagag gcagatgctg
4141atgactccaa cacccgtagc ctgcttctga gcaccaagca gctgttggcc gatatcatac
4201agttccatcc tggggacacc ctcaaggaga tcctgtccct ctcggcttcc agagagcaag
4261aagcagccca caagcagctg atgagccgac gccaggcctg tacagcccag acaccggagc
4321cactgcgacg acaccgctca ctgacagctc actccctcct gccactggca gagaagcagc
4381ggcgcgtcct gcggaacctg cgccgacttg aagccctggg gttggtcagc gccagaaatg
4441gctaccaggg gctagtggac gagctggcca aggacatccg caaccagcac agacacaggc
4501acaggcggaa ggcagagctg gtgaagctgc aggccacatt acagggcctg agcactaaga
4561ccaccttcta tgaggagcag ggtgactact acagccagta catccgggcc tgcctggacc
4621acctggcccc cgactccaag agttctggga aggggaagaa gcagccttct cttcattaca
4681ctgctgctca gcTCCTGGAA AAGGGTGTCT TGGTGGAAAT TGAAGATCTT CCCGCCTCTC
4741ACTTCAGAAA CGTCATCTTT GACATCACGC CGGGAGATGA GGCAGGAAAG TTTGAAGTAA
4801ATGCCAAGTT CCTGGGTGTG GACATGGAGC GATTTCAGCT TCACTATCAG GATCTCCTGC
4861AGCTCCAGTA TGAGGGTGTG GCTGTCATGA AACTCTTCAA CAAGGCCAAA GTCAATGTCA
4921ACCTTCTCAT CTTCCTCCTC AACAAGAAGT TTTTGCGGAA GTTGCCAACT TTCTTGTACA
4981AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT
5041AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA
5101AGGACGATCT GGAAACGCGA GTCAAAATAG GACGCGTTAA GTCgacaatc aacctctgga
5161ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)