Construct: ORF TRCN0000471666
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005929.1_s317c1
- Derived from:
- ccsbBroadEn_09482
- DNA Barcode:
- CATTTGTGGGTGGAGAATGTCTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SNX20 (124460)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471666
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 124460 | SNX20 | sorting nexin 20 | NM_001144972.2 | 99.6% | 99% | 104C>T |
| 2 | human | 124460 | SNX20 | sorting nexin 20 | NM_153337.3 | 77.5% | 74.8% | (many diffs) |
| 3 | human | 124460 | SNX20 | sorting nexin 20 | NM_182854.4 | 31.4% | 29.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 396
- ORF length:
- 306
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGCAAGTCC AGAGCACCCT GGGAGCCCTG 121 GCTGCATGGG ACCCATAACC CAGTGCACGG CAAGGACCCA GCAGGAAGCA CCAGCCACTG 181 GCCCCGACCT CCTGCACCCA GGACCTGACG GGCACTTAGA CACACACAGT GGCCTGAGCT 241 CCAACTCCAG CATGACCACG CGGGAGCTTC AGCAGTACTG GCAGAACCAG AAATGCCGCT 301 GGAAGCACGT CAAACTGCTC TTTGAGATCG CTTCAGCTCG CATCGAGGAG AGAAAAGTCT 361 CTAAGTTTGT GGCTCCGGCT CCTGACGCCA CACCCTGCCC AACTTTCTTG TACAAAGTGG 421 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 481 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 541 ACATTTGTGG GTGGAGAATG TCTACACGCG TTAAGTCgac aatcaacctc tggattacaa 601 aatttgtgaa agatt