Construct: ORF TRCN0000471676
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008298.1_s317c1
- Derived from:
- ccsbBroadEn_01142
- DNA Barcode:
- GGAACTCCACTAATAATCTAGCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PAFAH1B2 (5049)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471676
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5049 | PAFAH1B2 | platelet activating factor ... | NM_002572.4 | 100% | 100% | |
| 2 | human | 5049 | PAFAH1B2 | platelet activating factor ... | XM_017017840.1 | 94.6% | 94.6% | 1_39del |
| 3 | human | 5049 | PAFAH1B2 | platelet activating factor ... | NM_001309431.2 | 79% | 79% | 0_1ins144 |
| 4 | human | 5049 | PAFAH1B2 | platelet activating factor ... | NM_001184746.2 | 73.5% | 57.6% | (many diffs) |
| 5 | human | 5049 | PAFAH1B2 | platelet activating factor ... | NM_001184747.2 | 63.8% | 60.2% | (many diffs) |
| 6 | human | 5049 | PAFAH1B2 | platelet activating factor ... | NR_110268.2 | 60.6% | (many diffs) | |
| 7 | human | 5049 | PAFAH1B2 | platelet activating factor ... | NM_001184748.1 | 57.4% | 57.6% | 396_396delGins292 |
| 8 | human | 5049 | PAFAH1B2 | platelet activating factor ... | NR_110269.2 | 55.8% | (many diffs) | |
| 9 | human | 5049 | PAFAH1B2 | platelet activating factor ... | NR_110282.2 | 11.9% | (many diffs) | |
| 10 | mouse | 18475 | Pafah1b2 | platelet-activating factor ... | NM_008775.3 | 91.2% | 99.5% | (many diffs) |
| 11 | mouse | 18475 | Pafah1b2 | platelet-activating factor ... | XM_006510084.3 | 91.2% | 99.5% | (many diffs) |
| 12 | mouse | 18475 | Pafah1b2 | platelet-activating factor ... | XM_006510085.1 | 91.2% | 99.5% | (many diffs) |
| 13 | mouse | 18475 | Pafah1b2 | platelet-activating factor ... | XM_017313209.1 | 91.2% | 99.5% | (many diffs) |
| 14 | mouse | 18475 | Pafah1b2 | platelet-activating factor ... | XM_017313208.1 | 90% | 98.2% | (many diffs) |
| 15 | mouse | 18475 | Pafah1b2 | platelet-activating factor ... | XM_017313207.1 | 88.1% | 96.2% | (many diffs) |
| 16 | mouse | 18475 | Pafah1b2 | platelet-activating factor ... | XM_006510081.1 | 86.3% | 94.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 753
- ORF length:
- 687
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag ccaaggagac tcaaacccag cagctattcc gcatgcagca gaagatattc 121 aaggagatga ccgatggatg tctcagcaca acagatttgt tttggactgt aaagacaaag 181 agcctgatgt actgttcgtg ggagactcca tggtgcagtt aatgcagcaa tatgagatat 241 ggcgagagct tttttcccca cttcatgcac tgaattttgg aattggggga gatacaacaa 301 gacatgtttt gtggagacta aagaatggag aactggagaa tattaagcct aaggtcattg 361 ttGTCTGGGT AGGAACAAAT AACCACGAAA ATACAGCAGA AGAAGTAGCA GGTGGGATCG 421 AGGCCATTGT ACAACTTATC AACACAAGGC AGCCACAGGC CAAAATCATT GTATTGGGTT 481 TGTTACCTCG AGGTGAGAAA CCCAATCCTT TGAGGCAAAA GAACGCCAAG GTGAACCAAC 541 TCCTCAAGGT TTCGCTGCCG AAGCTTGCCA ACGTGCAGCT CCTGGATACC GACGGGGGTT 601 TTGTGCACTC GGACGGTGCC ATCTCCTGCC ACGACATGTT TGATTTTCTG CATCTGACAG 661 GAGGGGGCTA TGCAAAGATC TGCAAACCCC TGCATGAACT GATCATGCAG TTGTTGGAGG 721 AAACACCTGA GGAGAAACAA ACCACCATTG CCTGCCCAAC TTTCTTGTAC AAAGTGGTTG 781 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 841 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAGG 901 AACTCCACTA ATAATCTAGC CAACGCGTTA AGTCgacaat caacctctgg attacaaaat 961 ttgtgaaaga tt