Transcript: Human NR_110282.2

Homo sapiens platelet activating factor acetylhydrolase 1b catalytic subunit 2 (PAFAH1B2), transcript variant 7, non-coding RNA.

Source:
NCBI, updated 2019-07-31
Taxon:
Homo sapiens (human)
Gene:
PAFAH1B2 (5049)
Length:
3880
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_110282.2
NBCI Gene record:
PAFAH1B2 (5049)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_110282.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000048789 GAGGTGAGAAACCCAATCCTT pLKO.1 269 3UTR 100% 3.000 4.200 N PAFAH1B2 n/a
2 TRCN0000048792 GTAGGAACAAATAACCACGAA pLKO.1 148 3UTR 100% 2.640 2.112 N PAFAH1B2 n/a
3 TRCN0000218778 TGTCTGGGTAGGAACAAATAA pLKO_005 141 3UTR 100% 15.000 10.500 N PAFAH1B2 n/a
4 TRCN0000230675 CATTGCCTGACTGGCTCTTAT pLKO_005 525 3UTR 100% 13.200 9.240 N PAFAH1B2 n/a
5 TRCN0000218283 ATGTTCCTGGATGTTCATATC pLKO_005 636 3UTR 100% 10.800 7.560 N PAFAH1B2 n/a
6 TRCN0000048791 GCATGAACTGATCATGCAGTT pLKO.1 471 3UTR 100% 0.405 0.284 N PAFAH1B2 n/a
7 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 1883 3UTR 100% 5.625 2.813 Y KLHL30 n/a
8 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 1883 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_110282.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01142 pDONR223 100% 11.9% None (many diffs) n/a
2 ccsbBroad304_01142 pLX_304 0% 11.9% V5 (many diffs) n/a
3 TRCN0000471676 GGAACTCCACTAATAATCTAGCCA pLX_317 56.7% 11.9% V5 (many diffs) n/a
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