Construct: ORF TRCN0000471689
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016704.1_s317c1
- Derived from:
- ccsbBroadEn_12470
- DNA Barcode:
- ATCGTTCCATTTGAGTGTCTTTAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM168 (64418)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471689
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64418 | TMEM168 | transmembrane protein 168 | XM_024446869.1 | 73.4% | 73.4% | 0_1ins339 |
| 2 | human | 64418 | TMEM168 | transmembrane protein 168 | NM_001287497.2 | 61.1% | 61.1% | 1_813del |
| 3 | human | 64418 | TMEM168 | transmembrane protein 168 | NM_022484.6 | 61.1% | 61.1% | 1_813del |
| 4 | human | 64418 | TMEM168 | transmembrane protein 168 | XM_017012523.2 | 61.1% | 61.1% | 1_813del |
| 5 | human | 64418 | TMEM168 | transmembrane protein 168 | XR_001744854.2 | 31% | 1_1185del;1500_1689del;2654_4121del | |
| 6 | human | 64418 | TMEM168 | transmembrane protein 168 | NR_109840.2 | 17.3% | (many diffs) | |
| 7 | human | 64418 | TMEM168 | transmembrane protein 168 | XR_001744853.2 | 16.6% | (many diffs) | |
| 8 | human | 64418 | TMEM168 | transmembrane protein 168 | XM_017012524.2 | 16.1% | 15.1% | (many diffs) |
| 9 | mouse | 101118 | Tmem168 | transmembrane protein 168 | NM_001347059.1 | 65.8% | 70.4% | (many diffs) |
| 10 | mouse | 101118 | Tmem168 | transmembrane protein 168 | NM_028990.5 | 54.8% | 58.5% | (many diffs) |
| 11 | mouse | 101118 | Tmem168 | transmembrane protein 168 | XM_006504952.3 | 54.8% | 58.5% | (many diffs) |
| 12 | mouse | 101118 | Tmem168 | transmembrane protein 168 | XM_006504953.3 | 54.8% | 58.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1344
- ORF length:
- 1278
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat tgagcttaca ttttttattc tttccgcatt caaacttaga gacactcacc 121 tctggtattt tgtaatacct ggcttttcca tttttggaat tttctggatg atttgtcata 181 ttatttttct tttaactctt tggggattcc ataccaaatt aaatgactgc cataaagtat 241 attttactca caggacagat tacaatagcc ttgatagaat catggcatcc aaagggatgc 301 gccatttttg cttgatttca gagcagttgg tgttctttag tcttcttgca acagcgattt 361 tgggagcagt ttcctggcag ccaacaaatg gaattttctt gagcatgttt ctaatcgttt 421 tgccattgga atccatggct catgggctct tccatgaatt gggtaactgt ttaggaggaa 481 catctgttgg atatgctatt gtgattccca ccaacttctg cagtcctgat ggtcagccaa 541 cactgcttcc cccagaacat gtacaggagt taaatttgag gtctactggc atgctcaatg 601 ctatccaaag attttttgca tatcatatga ttgagaccta tggatgtgac tattccacaa 661 gtggactgtc atttgatact ctgcattcca aactaaaagc tttcctcgaa cttcggacag 721 tggatggacc cagacatgat acgtatattt tgtattacag tgggcacacc catggtacag 781 gagagtgggc tctagcaggt ggagatacac tacgccttga cacacttata gaatggtgga 841 gagaaaagaa tggttccttt tgttcccggc ttattatcGT ATTAGACAGC GAAAATTCAA 901 CCCCTTGGGT GAAAGAAGTG AGGAAAATTA ATGACCAGTA TATTGCAGTG CAAGGAGCAG 961 AGTTGATAAA AACAGTAGAT ATTGAAGAAG CTGACCCGCC ACAGCTAGGT GACTTTACAA 1021 AAGACTGGGT AGAATATAAC TGCAACTCCA GTAATAACAT CTGCTGGACT GAAAAGGGAC 1081 GCACAGTGAA AGCAGTATAT GGTGTGTCAA AACGGTGGAG TGACTACACT CTGCATTTGC 1141 CAACGGGAAG CGATGTGGCC AAGCACTGGA TGTTACACTT TCCTCGTATT ACATATCCCC 1201 TAGTGCATTT GGCAAATTGG TTATGCGGTC TGAACCTTTT TTGGATCTGC AAAACTTGTT 1261 TTAGGTGCTT GAAAAGATTA AAAATGAGTT GGTTTCTTCC TACTGTGCTG GACACAGGAC 1321 AAGGCTTCAA ACTTGTCAAA TCTTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1381 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1441 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA TCGTTCCATT 1501 TGAGTGTCTT TATACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1561 att