Construct: ORF TRCN0000471699
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006161.1_s317c1
- Derived from:
- ccsbBroadEn_04477
- DNA Barcode:
- CCCGAAATCCCCCGATGCCCATTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LGALS12 (85329)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471699
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 85329 | LGALS12 | galectin 12 | NM_033101.4 | 93.4% | 93.4% | 0_1ins66 |
| 2 | human | 85329 | LGALS12 | galectin 12 | NM_001142535.2 | 93.1% | 93.1% | 0_1ins66;157_159delAGT |
| 3 | human | 85329 | LGALS12 | galectin 12 | NM_001142536.2 | 90.7% | 90.7% | 0_1ins66;531_532ins27 |
| 4 | human | 85329 | LGALS12 | galectin 12 | NM_001142537.2 | 81.8% | 81.8% | 0_1ins183 |
| 5 | human | 85329 | LGALS12 | galectin 12 | NM_001142538.2 | 79.1% | 79.1% | 0_1ins183;414_415ins27 |
| 6 | human | 85329 | LGALS12 | galectin 12 | XM_017018458.1 | 74.4% | 69.8% | 223_225delAGT;716_717ins139;756_757ins116 |
| 7 | human | 85329 | LGALS12 | galectin 12 | XR_950087.2 | 56.6% | (many diffs) | |
| 8 | human | 85329 | LGALS12 | galectin 12 | XR_950085.2 | 55.5% | (many diffs) | |
| 9 | human | 85329 | LGALS12 | galectin 12 | XR_950086.1 | 53.3% | (many diffs) | |
| 10 | mouse | 56072 | Lgals12 | lectin, galactose binding, ... | NM_019516.3 | 79.5% | 75.8% | (many diffs) |
| 11 | mouse | 56072 | Lgals12 | lectin, galactose binding, ... | XM_006527219.3 | 79.5% | 75.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1074
- ORF length:
- 1008
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag tcagcccagt gggggcaggg ctcctggaac gaggatctac agttggagtt 121 gccccactgt catgtcacct ggagaaaaac tggacccaat tcctgacagc ttcattctgc 181 aaccaccagt cttccacccg gtggttcctt atgtcacgac gatttttgga ggcctgcatg 241 caggcaagat ggtcatgctg caaggagtgg tccctctaga tgcacacagg tttcaggtgg 301 acttccagtg tggctgcagc ctgtgtcccc ggccagatat cgccttccac ttcaaccctc 361 gcttccatac caccaagccc catgtcatct gcaacaccct gcatggtgga cgctggcaaa 421 gggaggcccg gtggccccac ctggccctgc gaagaggctc cagcttcctc atcctctttc 481 tcttcgggaa tgaggaagtg aaggtgagtg tgaatggaca gcactttctc cacttccgct 541 accggctccc actgtctcat gtggacacgc tgggtatatt tggtgacatc ctggtagagg 601 ctgttggatt cctgaacatc aatccatttg tggagggcag cagagagtac ccagctggac 661 atcctttcct gctgatgagc cccaggctgg aggtgccctg ctcacatgcT CTTCCCCAGG 721 GTCTCTCGCC TGGGCAGGTC ATCATAGTAC GGGGACTGGT CTTGCAAGAG CCGAAGCATT 781 TTACTGTGAG CCTGAGGGAC CAGGCTGCCC ATGCTCCTGT GACACTCAGG GCCTCCTTCG 841 CAGACAGAAC TCTGGCCTGG ATCTCCCGCT GGGGGCAGAA GAAACTGATC TCAGCCCCCT 901 TCCTCTTTTA CCCCCAGAGA TTCTTTGAGG TGCTGCTCCT GTTCCAGGAG GGAGGGCTGA 961 AGCTGGCGCT CAATGGGCAG GGGCTGGGGG CCACCAGCAT GAACCAGCAG GCCCTGGAGC 1021 AGCTGCGGGA GCTCCGGATC AGTGGAAGTG TCCAGCTCTA CTGTGTCCAC TCCTGCCCAA 1081 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1141 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1201 TATCTTGTGG AAAGGACGAC CCGAAATCCC CCGATGCCCA TTTACGCGTT AAGTCgacaa 1261 tcaacctctg gattacaaaa tttgtgaaag att