Construct: ORF TRCN0000471702
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007286.1_s317c1
- Derived from:
- ccsbBroadEn_14169
- DNA Barcode:
- GGCAACGCACCATAGGTCCTAAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GRAMD1C (54762)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471702
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54762 | GRAMD1C | GRAM domain containing 1C | XM_017006647.1 | 82% | 82% | 1_210del |
| 2 | human | 54762 | GRAMD1C | GRAM domain containing 1C | XM_017006648.1 | 82% | 82% | 1_210del |
| 3 | human | 54762 | GRAMD1C | GRAM domain containing 1C | XM_017006649.1 | 82% | 82% | 1_210del |
| 4 | human | 54762 | GRAMD1C | GRAM domain containing 1C | NM_001172105.2 | 70.2% | 70.2% | 1_408del |
| 5 | human | 54762 | GRAMD1C | GRAM domain containing 1C | XM_011512931.1 | 70.2% | 70.2% | 1_408del |
| 6 | human | 54762 | GRAMD1C | GRAM domain containing 1C | XM_005247546.2 | 50.9% | 50.9% | 1_927del |
| 7 | human | 54762 | GRAMD1C | GRAM domain containing 1C | XM_011512930.1 | 49.2% | 49.2% | 1_993del |
| 8 | human | 54762 | GRAMD1C | GRAM domain containing 1C | NM_017577.5 | 48.4% | 48.4% | 1_1023del |
| 9 | human | 54762 | GRAMD1C | GRAM domain containing 1C | XM_017006646.1 | 41.5% | 41.5% | 1_927del;1359_1360ins177 |
| 10 | human | 54762 | GRAMD1C | GRAM domain containing 1C | XM_005247547.2 | 39.5% | 39.5% | 1_1023del;1455_1456ins177 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1029
- ORF length:
- 963
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt tgaattgctc tttaccagtt cacgctttat gcagaaattt gccagttcta 121 gaaatataat agatgtagta tctacccctt ggactgcaga acttggaggt gatcagctga 181 gaacgatgac ctacactata gtccttaata gtccacttac tggaaaatgc actgctgcca 241 ctgaaaagca gacactgtat aaagaaagtc gggaagcacg attttatttg gtagattcag 301 aagtactgac acatgatgtc ccctaccatg attacttcta taccgtgaac agatactgta 361 tcatccgatc ttcaaaacag aaatgcaggc taagagtttc cacagatttg aaatacagaa 421 aacagccatg gggccttgtc aaatctttaa ttgaaaagaa ttcctggagt tctttggagg 481 actatttcaa acagcttgaa tcagatttgt taattgaaga atctgtatta aatcaggcca 541 ttgaagaccc tggaaaactt actggcctac gaaggagaag gcgaaccttc aaccgaacag 601 cagaaacagt tcctaaactt tcctctcagc attcctctgg agatgtgggc ttaggtgcca 661 aaggggatat taCAGGAAAG AAAAAGGAAA TGGAAAACTA TAACGTCACT CTTATTGTGG 721 TAATGAGTAT TTTTGTGTTG TTATTAGTTT TGTTGAATGT GACACTGTTT CTGAAGCTGT 781 CAAAGATAGA ACATGCTGCT CAGTCCTTTT ACCGTCTCCG CCTCCAAGAA GAGAAATCTT 841 TAAATTTAGC CTCTGATATG GTGTCAAGAG CAGAAACTAT TCAGAAGAAT AAAGATCAGG 901 CCCATCGTTT AAAGGGAGTG CTCCGAGACT CCATAGTGAT GCTTGAACAG CTGAAGAGCT 961 CACTCATTAT GCTTCAGAAA ACGTTTGATC TACTAAATAA GAATAAGACT GGCATGGCTG 1021 TTGAAAGCTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1081 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1141 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGGCAAC GCACCATAGG TCCTAAACAC 1201 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt