Construct: ORF TRCN0000471719
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015180.1_s317c1
- Derived from:
- ccsbBroadEn_03412
- DNA Barcode:
- GGATTGCCTGATCACACAGGATAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HAO1 (54363)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471719
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54363 | HAO1 | hydroxyacid oxidase 1 | NM_017545.3 | 100% | 100% | |
| 2 | mouse | 15112 | Hao1 | hydroxyacid oxidase 1, liver | NM_010403.2 | 87.2% | 89.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1179
- ORF length:
- 1110
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gctcccccgg ctaatttgta tcaatgatta tgaacaacat gctaaatcag 121 tacttccaaa gtctatatat gactattaca ggtctggggc aaatgatgaa gaaactttgg 181 ctgataatat tgcagcattt tccagatgga agctgtatcc aaggatgctc cggaatgttg 241 ctgaaacaga tctgtcgact tctgttttag gacagagggt cagcatgcca atatgtgtgg 301 gggctacggc catgcagcgc atggctcatg tggacggcga gcttgccact gtgagagcct 361 gtcagtccct gggaacgggc atgatgttga gttcctgggc cacctcctca attgaagaag 421 tggcggaagc tggtcctgag gcacttcgtt ggctgcaact gtatatctac aaggaccgag 481 aagtcaccaa gaagctagtg cggcaggcag agaagatggg ctacaaggcc atatttgtga 541 cagtggacac accttacctg ggcaaccgtc tggatgatgt gcgtaacaga ttcaaactgc 601 cgccacaact caggatgaaa aattttgaaa ccagtacttt atcattttct cctgaggaaa 661 attttggaga cgacagtgga cttgctgcat atgtggctaa agcaatagac ccatctatca 721 gctgggaaga tatcaaatgg ctgagaagac tgacaTCATT GCCAATTGTT GCAAAGGGCA 781 TTTTGAGAGG TGATGATGCC AGGGAGGCTG TTAAACATGG CTTGAATGGG ATCTTGGTGT 841 CGAATCATGG GGCTCGACAA CTCGATGGGG TGCCAGCCAC TATTGATGTT CTGCCAGAAA 901 TTGTGGAGGC TGTGGAAGGG AAGGTGGAAG TCTTCCTGGA CGGGGGTGTG CGGAAAGGCA 961 CTGATGTTCT GAAAGCTCTG GCTCTTGGCG CCAAGGCTGT GTTTGTGGGG AGACCAATCG 1021 TTTGGGGCTT AGCTTTCCAG GGGGAGAAAG GTGTTCAAGA TGTCCTCGAG ATACTAAAGG 1081 AAGAATTCCG GTTGGCCATG GCTCTGAGTG GGTGCCAGAA TGTGAAAGTC ATCGACAAGA 1141 CATTGGTGAG GAAAAATCCT TTGGCCGTTT CCAAGATCTT GCCAACTTTC TTGTACAAAG 1201 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1261 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1321 ACGAGGATTG CCTGATCACA CAGGATACAC GCGTTAAGTC gacaatcaac ctctggatta 1381 caaaatttgt gaaagatt