Construct: ORF TRCN0000471728

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003671.1_s317c1
Derived from:
ccsbBroadEn_12463
DNA Barcode:
TGTGATCACACCCGAAAGTGGTTC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ATL2 (64225)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000471728
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 64225 ATL2 atlastin GTPase 2 NM_001330458.1 99.9% 100% 453A>C
2 human 64225 ATL2 atlastin GTPase 2 NM_001330460.1 91.9% 91.7% (many diffs)
3 human 64225 ATL2 atlastin GTPase 2 NM_001330461.2 91.9% 91.7% (many diffs)
4 human 64225 ATL2 atlastin GTPase 2 NM_001330464.2 86.3% 86.4% 0_1ins168;285A>C
5 human 64225 ATL2 atlastin GTPase 2 NM_001308076.1 72.8% 72.9% 1_459del;912A>C
6 human 64225 ATL2 atlastin GTPase 2 NM_001135673.4 70.6% 70.6% 1_513del;966A>C
7 human 64225 ATL2 atlastin GTPase 2 NM_001330459.1 67.6% 66.9% (many diffs)
8 human 64225 ATL2 atlastin GTPase 2 NM_022374.5 66.8% 65.1% (many diffs)
9 human 64225 ATL2 atlastin GTPase 2 NM_001330462.1 66.1% 65.4% (many diffs)
10 human 64225 ATL2 atlastin GTPase 2 NM_001330463.2 65.6% 64.8% (many diffs)
11 mouse 56298 Atl2 atlastin GTPase 2 NM_178050.4 91% 98.7% (many diffs)
12 mouse 56298 Atl2 atlastin GTPase 2 XM_006524657.3 89.9% 97.6% (many diffs)
13 mouse 56298 Atl2 atlastin GTPase 2 NM_001286647.1 85% 90.5% (many diffs)
14 mouse 56298 Atl2 atlastin GTPase 2 XM_006524656.2 70.2% 76.2% (many diffs)
15 mouse 56298 Atl2 atlastin GTPase 2 XM_017317584.1 66.6% 70.5% (many diffs)
16 mouse 56298 Atl2 atlastin GTPase 2 NM_019717.3 64.3% 69.8% (many diffs)
17 mouse 56298 Atl2 atlastin GTPase 2 XM_006524653.2 63.7% 69.2% (many diffs)
18 mouse 56298 Atl2 atlastin GTPase 2 XM_006524655.3 60.6% 64.3% (many diffs)
19 mouse 56298 Atl2 atlastin GTPase 2 XM_006524654.3 60.5% 64% (many diffs)
20 mouse 56298 Atl2 atlastin GTPase 2 XR_001782092.1 22.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1302
ORF length:
1236
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga tacccagggt gcctttgata gccagtcaac tatcaaagac tgtgcaacgg
121tgtttgctct gagcactatg actagctctg tccaggtata taatctgtct cagaatattc
181aagaagatga tcttcaacat ttgcaattat ttacagagta tggaagactt gcgatggaag
241aaatctacca gaaaccattt cagacattaa tgtttttgat tcgagattgg agctatcctt
301atgaacattc atatggtttg gaaggtggaa agcaatttct tgaaaagaga ttacaggtaa
361aacaaaatca acatgaagag cttcagaatg taaggaagca catacacaat tgtttctcaa
421atcttggttg cttccttttg ccacatcctg gtcttaaagt tgcaactaat cctagttttg
481atgggagatt gaaagatatt gatgaagact ttaaacgcga gcttcgaaat ctggttccat
541tgctgcttgc ccctgaaaat ttggtagaaa aagagataag tggatctaaa gtcacttgta
601gagatcttgt agaatatttt aaggcttaca tcaaaatcta tcaaggagaa gaacttccac
661atccaaagtc catgcttcag gcaacagctg aagctaataa tcttgctgca gtagcaggag
721caagagatac ctattgtaaa agtatggaac aggtatgtgg aggggacaag ccttacattg
781caccttcaga tctggagcga aaacacttgg atctcaagga agtggcgata aaacaatttc
841gttcagtaaa aaagatgggt ggagatgagt tctgccgtcg ttatcaggac cagcttgaag
901ctgaaattga agaaacctat gcaaatttta taaagcacaa tgatggcaaa aatatcttct
961atgctgctcg taccccagcc acactgtttg cggtcatgtt TGCTATGTAT ATAATCTCAG
1021GACTGACTGG CTTCATTGGC CTAAACTCTA TAGCTGTCTT GTGTAACCTT GTCATGGGGT
1081TAGCACTGAT ATTTCTTTGT ACTTGGGCAT ATGTTAAATA CTCTGGGGAG TTCAGAGAAA
1141TTGGAACAGT GATTGATCAG ATTGCTGAAA CACTATGGGA ACAGGTATTG AAGCCCCTGG
1201GTGATAATTT GATGGAGGAA AACATAAGGC AGTCTGTAAC AAACTCTATC AAAGCAGGCC
1261TGACTGACCA GGTGTCTCAT CATGCCAGAT TAAAGACAGA CTGCCCAACT TTCTTGTACA
1321AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT
1381AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA
1441AGGACGATGT GATCACACCC GAAAGTGGTT CACGCGTTAA GTCgacaatc aacctctgga
1501ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)