Construct: ORF TRCN0000471728
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003671.1_s317c1
- Derived from:
- ccsbBroadEn_12463
- DNA Barcode:
- TGTGATCACACCCGAAAGTGGTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATL2 (64225)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471728
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64225 | ATL2 | atlastin GTPase 2 | NM_001330458.1 | 99.9% | 100% | 453A>C |
| 2 | human | 64225 | ATL2 | atlastin GTPase 2 | NM_001330460.1 | 91.9% | 91.7% | (many diffs) |
| 3 | human | 64225 | ATL2 | atlastin GTPase 2 | NM_001330461.2 | 91.9% | 91.7% | (many diffs) |
| 4 | human | 64225 | ATL2 | atlastin GTPase 2 | NM_001330464.2 | 86.3% | 86.4% | 0_1ins168;285A>C |
| 5 | human | 64225 | ATL2 | atlastin GTPase 2 | NM_001308076.1 | 72.8% | 72.9% | 1_459del;912A>C |
| 6 | human | 64225 | ATL2 | atlastin GTPase 2 | NM_001135673.4 | 70.6% | 70.6% | 1_513del;966A>C |
| 7 | human | 64225 | ATL2 | atlastin GTPase 2 | NM_001330459.1 | 67.6% | 66.9% | (many diffs) |
| 8 | human | 64225 | ATL2 | atlastin GTPase 2 | NM_022374.5 | 66.8% | 65.1% | (many diffs) |
| 9 | human | 64225 | ATL2 | atlastin GTPase 2 | NM_001330462.1 | 66.1% | 65.4% | (many diffs) |
| 10 | human | 64225 | ATL2 | atlastin GTPase 2 | NM_001330463.2 | 65.6% | 64.8% | (many diffs) |
| 11 | mouse | 56298 | Atl2 | atlastin GTPase 2 | NM_178050.4 | 91% | 98.7% | (many diffs) |
| 12 | mouse | 56298 | Atl2 | atlastin GTPase 2 | XM_006524657.3 | 89.9% | 97.6% | (many diffs) |
| 13 | mouse | 56298 | Atl2 | atlastin GTPase 2 | NM_001286647.1 | 85% | 90.5% | (many diffs) |
| 14 | mouse | 56298 | Atl2 | atlastin GTPase 2 | XM_006524656.2 | 70.2% | 76.2% | (many diffs) |
| 15 | mouse | 56298 | Atl2 | atlastin GTPase 2 | XM_017317584.1 | 66.6% | 70.5% | (many diffs) |
| 16 | mouse | 56298 | Atl2 | atlastin GTPase 2 | NM_019717.3 | 64.3% | 69.8% | (many diffs) |
| 17 | mouse | 56298 | Atl2 | atlastin GTPase 2 | XM_006524653.2 | 63.7% | 69.2% | (many diffs) |
| 18 | mouse | 56298 | Atl2 | atlastin GTPase 2 | XM_006524655.3 | 60.6% | 64.3% | (many diffs) |
| 19 | mouse | 56298 | Atl2 | atlastin GTPase 2 | XM_006524654.3 | 60.5% | 64% | (many diffs) |
| 20 | mouse | 56298 | Atl2 | atlastin GTPase 2 | XR_001782092.1 | 22.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1302
- ORF length:
- 1236
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tacccagggt gcctttgata gccagtcaac tatcaaagac tgtgcaacgg 121 tgtttgctct gagcactatg actagctctg tccaggtata taatctgtct cagaatattc 181 aagaagatga tcttcaacat ttgcaattat ttacagagta tggaagactt gcgatggaag 241 aaatctacca gaaaccattt cagacattaa tgtttttgat tcgagattgg agctatcctt 301 atgaacattc atatggtttg gaaggtggaa agcaatttct tgaaaagaga ttacaggtaa 361 aacaaaatca acatgaagag cttcagaatg taaggaagca catacacaat tgtttctcaa 421 atcttggttg cttccttttg ccacatcctg gtcttaaagt tgcaactaat cctagttttg 481 atgggagatt gaaagatatt gatgaagact ttaaacgcga gcttcgaaat ctggttccat 541 tgctgcttgc ccctgaaaat ttggtagaaa aagagataag tggatctaaa gtcacttgta 601 gagatcttgt agaatatttt aaggcttaca tcaaaatcta tcaaggagaa gaacttccac 661 atccaaagtc catgcttcag gcaacagctg aagctaataa tcttgctgca gtagcaggag 721 caagagatac ctattgtaaa agtatggaac aggtatgtgg aggggacaag ccttacattg 781 caccttcaga tctggagcga aaacacttgg atctcaagga agtggcgata aaacaatttc 841 gttcagtaaa aaagatgggt ggagatgagt tctgccgtcg ttatcaggac cagcttgaag 901 ctgaaattga agaaacctat gcaaatttta taaagcacaa tgatggcaaa aatatcttct 961 atgctgctcg taccccagcc acactgtttg cggtcatgtt TGCTATGTAT ATAATCTCAG 1021 GACTGACTGG CTTCATTGGC CTAAACTCTA TAGCTGTCTT GTGTAACCTT GTCATGGGGT 1081 TAGCACTGAT ATTTCTTTGT ACTTGGGCAT ATGTTAAATA CTCTGGGGAG TTCAGAGAAA 1141 TTGGAACAGT GATTGATCAG ATTGCTGAAA CACTATGGGA ACAGGTATTG AAGCCCCTGG 1201 GTGATAATTT GATGGAGGAA AACATAAGGC AGTCTGTAAC AAACTCTATC AAAGCAGGCC 1261 TGACTGACCA GGTGTCTCAT CATGCCAGAT TAAAGACAGA CTGCCCAACT TTCTTGTACA 1321 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1381 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1441 AGGACGATGT GATCACACCC GAAAGTGGTT CACGCGTTAA GTCgacaatc aacctctgga 1501 ttacaaaatt tgtgaaagat t