Construct: ORF TRCN0000471744
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011756.1_s317c1
- Derived from:
- ccsbBroadEn_13832
- DNA Barcode:
- ACAAGTCACTCGCGCGCAGACCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AP1S1 (1174)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471744
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1174 | AP1S1 | adaptor related protein com... | NM_001283.5 | 78.1% | 79.7% | 14T>G;382_383insCTTTCCCCTTTTCCC;384_474delinsC |
| 2 | mouse | 11769 | Ap1s1 | adaptor protein complex AP-... | NM_007457.2 | 74.1% | 79.7% | (many diffs) |
| 3 | mouse | 11769 | Ap1s1 | adaptor protein complex AP-... | XM_006504348.3 | 66.2% | 71% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 489
- ORF length:
- 399
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGATGCGGTT CAGGCTATTA TTCAGCCGGC 121 AGGGAAAACT GCGGCTGCAA AAATGGTACC TGGCCACTTC GGACAAGGAA CGGAAGAAGA 181 TGGTGCGCGA GCTCATGCAG GTTGTCCTGG CTCGAAAGCC CAAGATGTGC AGCTTCCTGG 241 AGTGGAGGGA CCTCAAAGTT GTCTATAAGA GATATGCCAG CCTCTACTTC TGCTGCGCCA 301 TCGAGGGCCA AGACAATGAG CTCATCACAC TGGAGCTGAT CCACCGATAC GTGGAGCTCT 361 TAGACAAATA CTTTGGCAGT GTGTGCGAGC TGGACATCAT CTTCAACTTT GAGAAGGCCT 421 ACTTCATCCT GGATGAGTTT TTGATGGGGG GGGATGTCCA GGACACCTCC ACTTTCCCCT 481 TTTCCCACTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 541 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 601 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAACAAGT CACTCGCGCG CAGACCCGAC 661 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt