Construct: ORF TRCN0000471745
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014998.1_s317c1
- Derived from:
- ccsbBroadEn_09016
- DNA Barcode:
- CAATTCAAGCAATGTCCCCGTCTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ACSF2 (80221)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471745
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80221 | ACSF2 | acyl-CoA synthetase family ... | NM_025149.5 | 99.8% | 99.8% | 224G>T;1326C>G |
| 2 | human | 80221 | ACSF2 | acyl-CoA synthetase family ... | NM_001288969.1 | 97.7% | 97.7% | 224G>T;434_435ins39;1287C>G |
| 3 | human | 80221 | ACSF2 | acyl-CoA synthetase family ... | NM_001288968.1 | 95.9% | 95.9% | 129_203del;299G>T;1401C>G |
| 4 | human | 80221 | ACSF2 | acyl-CoA synthetase family ... | NM_001288970.1 | 92.8% | 92.8% | 224G>T;323_324ins129;1197C>G |
| 5 | human | 80221 | ACSF2 | acyl-CoA synthetase family ... | XM_011525294.2 | 91.2% | 90.1% | (many diffs) |
| 6 | human | 80221 | ACSF2 | acyl-CoA synthetase family ... | XR_429924.3 | 83% | (many diffs) | |
| 7 | human | 80221 | ACSF2 | acyl-CoA synthetase family ... | XR_934565.2 | 81.6% | (many diffs) | |
| 8 | human | 80221 | ACSF2 | acyl-CoA synthetase family ... | NM_001288971.1 | 73.9% | 73.9% | 0_1ins480;846C>G |
| 9 | human | 80221 | ACSF2 | acyl-CoA synthetase family ... | NM_001288972.1 | 73.9% | 73.9% | 0_1ins480;846C>G |
| 10 | human | 80221 | ACSF2 | acyl-CoA synthetase family ... | XM_006722110.4 | 71.9% | 71.5% | (many diffs) |
| 11 | human | 80221 | ACSF2 | acyl-CoA synthetase family ... | XR_934563.3 | 69.1% | (many diffs) | |
| 12 | human | 80221 | ACSF2 | acyl-CoA synthetase family ... | XR_934566.3 | 66.9% | (many diffs) | |
| 13 | human | 80221 | ACSF2 | acyl-CoA synthetase family ... | XR_934564.2 | 64% | (many diffs) | |
| 14 | human | 80221 | ACSF2 | acyl-CoA synthetase family ... | XR_934567.2 | 59.5% | (many diffs) | |
| 15 | human | 80221 | ACSF2 | acyl-CoA synthetase family ... | NR_110232.1 | 26% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1911
- ORF length:
- 1845
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgtctacgtc gggatgctgc gcctggggag gctgtgcgcc gggagctcgg 121 gggtgctggg ggcccgggcc gccctctctc ggagttggca ggaagccagg ttgcagggtg 181 tccgcttcct cagttccaga gaggtggatc gcatggtctc cacgcccatc ggaggcctca 241 gctacgttca ggggtgcacc aaaaagcatc ttaacagcaa gactgtggtc cagtgcctgg 301 agaccacagc acagagggtc ccagaacgag aggccttggt cgtcctccat gaagacgtca 361 ggttgacctt tgcccaactc aaggaggagg tggacaaagc tgcttctggc ctcctgagca 421 ttggcctctg caaaggtgac cggctgggca tgtggggacc taactcctat gcatgggtgc 481 tcatgcagtt ggccaccgcc caggcgggca tcattctggt gtctgtgaac ccagcctacc 541 aggctatgga actggagtat gtcctcaaga aggtgggctg caaggccctt gtgttcccca 601 agcaattcaa gacccagcaa tactacaacg tcctgaagca gatctgtcca gaagtggaga 661 atgcccagcc aggggccttg aagagtcaga ggctcccaga tctgaccaca gtcatctcgg 721 tggatgcccc tttgccgggg accctgctcc tggatgaagt ggtggcggct ggcagcacac 781 ggcagcatct ggaccagctc caatacaacc agcagttcct gtcctgccat gaccccatca 841 acatccagtt cacctcgggg acaacaggca gccccaaggg ggccaccctc tcccactaca 901 acattgtcaa caactccaac attttaggag agcgcctgaa actgcatgag aagacaccag 961 agcagttgcg gatgatcctg cccaaccccc tgtaccattg cctgggttcc gtggcaggca 1021 caatgatgtg tctgatgtac ggtgccaccc tcatcctggc ctctcccatc ttcaatggca 1081 agaaggcact ggaggccatc agcagagaga gaggcacctt cctgtatggt acccccacga 1141 tgttcgtgga cattctgaac cagccagact tctccagtta tgacatctcg accatgtgtg 1201 gaggtgtcat tgctgggtcc cctgcacctc cagagttgat ccgagccatc atcaacaaga 1261 taaatatgaa ggacctggtg gttgcttatg gaaccacaga gaacagtccc gtgacattcg 1321 cgcacttccc tgaggacact gtggagcaga aggcagaaag cgtgggcaga attatgcctc 1381 acacggaggc gcggatcatg aacatggagg cagggacgct ggcaaagctg aacacgcccg 1441 gggagctgtg catccgaggg tacTGCGTCA TGCTGGGCTA CTGGGGTGAG CCTCAGAAGA 1501 CAGAGGAAGC AGTGGATCAG GACAAGTGGT ATTGGACAGG AGATGTCGCC ACAATGAATG 1561 AGCAGGGCTT CTGCAAGATC GTGGGCCGCT CTAAGGATAT GATCATCCGG GGTGGTGAGA 1621 ACATCTACCC CGCAGAGCTC GAGGACTTCT TTCACACACA CCCGAAGGTG CAGGAAGTGC 1681 AGGTGGTGGG AGTGAAGGAC GATCGGATGG GGGAAGAGAT TTGTGCCTGC ATTCGGCTGA 1741 AGGACGGGGA GGAGACCACG GTGGAGGAGA TAAAAGCTTT CTGCAAAGGG AAGATCTCTC 1801 ACTTCAAGAT TCCGAAGTAC ATCGTGTTTG TCACAAACTA CCCCCTCACC ATTTCAGGAA 1861 AGATCCAGAA ATTCAAACTT CGAGAGCAGA TGGAACGACA TCTAAATCTG TGCCCAACTT 1921 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1981 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 2041 CTTGTGGAAA GGACGACAAT TCAAGCAATG TCCCCGTCTA ACGCGTTAAG TCgacaatca 2101 acctctggat tacaaaattt gtgaaagatt