Construct: ORF TRCN0000471752
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001397.1_s317c1
- Derived from:
- BRDN0000395110
- DNA Barcode:
- ACGCAATGCTAACAACCAAAGATG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- FN1 (2335)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471752
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2335 | FN1 | fibronectin 1 | NM_212474.2 | 99.8% | 4.5% | (many diffs) |
| 2 | human | 2335 | FN1 | fibronectin 1 | NM_001306132.1 | 97% | 4.4% | (many diffs) |
| 3 | human | 2335 | FN1 | fibronectin 1 | XM_005246416.1 | 96.2% | 4.4% | (many diffs) |
| 4 | human | 2335 | FN1 | fibronectin 1 | NM_001306131.1 | 95.9% | 4.3% | (many diffs) |
| 5 | human | 2335 | FN1 | fibronectin 1 | NM_001365524.1 | 95.8% | 4.3% | (many diffs) |
| 6 | human | 2335 | FN1 | fibronectin 1 | NM_001306130.1 | 95.8% | 4.3% | (many diffs) |
| 7 | human | 2335 | FN1 | fibronectin 1 | NM_001365523.1 | 95.6% | 4.3% | (many diffs) |
| 8 | human | 2335 | FN1 | fibronectin 1 | XM_005246411.1 | 95.1% | 4.3% | (many diffs) |
| 9 | human | 2335 | FN1 | fibronectin 1 | NM_212476.2 | 94.6% | 4.3% | (many diffs) |
| 10 | human | 2335 | FN1 | fibronectin 1 | XM_017003695.1 | 93.9% | 4.3% | (many diffs) |
| 11 | human | 2335 | FN1 | fibronectin 1 | NM_212478.2 | 93.2% | 4.2% | (many diffs) |
| 12 | human | 2335 | FN1 | fibronectin 1 | NM_001365522.1 | 93.1% | 4.2% | (many diffs) |
| 13 | human | 2335 | FN1 | fibronectin 1 | XM_005246410.1 | 92.5% | 4.2% | (many diffs) |
| 14 | human | 2335 | FN1 | fibronectin 1 | NM_002026.3 | 92.2% | 4.2% | (many diffs) |
| 15 | human | 2335 | FN1 | fibronectin 1 | NM_001365521.1 | 92.2% | 4.2% | (many diffs) |
| 16 | human | 2335 | FN1 | fibronectin 1 | XM_017003692.1 | 92.1% | 4.2% | (many diffs) |
| 17 | human | 2335 | FN1 | fibronectin 1 | NM_001365520.1 | 92% | 4.1% | (many diffs) |
| 18 | human | 2335 | FN1 | fibronectin 1 | NM_001365519.1 | 91.9% | 4.1% | (many diffs) |
| 19 | human | 2335 | FN1 | fibronectin 1 | XM_005246408.1 | 91.5% | 4.1% | (many diffs) |
| 20 | human | 2335 | FN1 | fibronectin 1 | XM_005246407.1 | 91.4% | 4.1% | (many diffs) |
| 21 | human | 2335 | FN1 | fibronectin 1 | XM_024452770.1 | 91.2% | 4.1% | (many diffs) |
| 22 | human | 2335 | FN1 | fibronectin 1 | NM_001365518.1 | 91% | 4.1% | (many diffs) |
| 23 | human | 2335 | FN1 | fibronectin 1 | NM_001365517.1 | 91% | 4.1% | (many diffs) |
| 24 | human | 2335 | FN1 | fibronectin 1 | XM_005246404.1 | 90.3% | 4.1% | (many diffs) |
| 25 | human | 2335 | FN1 | fibronectin 1 | XM_005246403.1 | 90.3% | 4.1% | (many diffs) |
| 26 | human | 2335 | FN1 | fibronectin 1 | XM_005246402.1 | 89.7% | 4% | (many diffs) |
| 27 | human | 2335 | FN1 | fibronectin 1 | XM_005246401.1 | 89% | 4% | (many diffs) |
| 28 | human | 2335 | FN1 | fibronectin 1 | NM_001306129.1 | 88.8% | 4% | (many diffs) |
| 29 | human | 2335 | FN1 | fibronectin 1 | XM_024452769.1 | 88.5% | 4% | (many diffs) |
| 30 | human | 2335 | FN1 | fibronectin 1 | XM_005246399.1 | 88.1% | 4% | (many diffs) |
| 31 | human | 2335 | FN1 | fibronectin 1 | XM_005246398.1 | 87.9% | 4% | (many diffs) |
| 32 | human | 2335 | FN1 | fibronectin 1 | NM_212482.3 | 87.7% | 3.9% | (many diffs) |
| 33 | human | 2335 | FN1 | fibronectin 1 | XM_005246397.1 | 87% | 3.9% | (many diffs) |
| 34 | human | 2335 | FN1 | fibronectin 1 | NM_054034.2 | 30% | 15% | (many diffs) |
| 35 | mouse | 14268 | Fn1 | fibronectin 1 | NM_001276413.1 | 86.4% | 4.2% | (many diffs) |
| 36 | mouse | 14268 | Fn1 | fibronectin 1 | NM_001276412.1 | 83% | 4% | (many diffs) |
| 37 | mouse | 14268 | Fn1 | fibronectin 1 | XM_017315837.1 | 83% | 4% | (many diffs) |
| 38 | mouse | 14268 | Fn1 | fibronectin 1 | NM_001276411.1 | 82.8% | 4% | (many diffs) |
| 39 | mouse | 14268 | Fn1 | fibronectin 1 | NM_001276410.1 | 81.9% | 4% | (many diffs) |
| 40 | mouse | 14268 | Fn1 | fibronectin 1 | XM_017315848.1 | 79.9% | 4.1% | (many diffs) |
| 41 | mouse | 14268 | Fn1 | fibronectin 1 | XM_006495700.3 | 79.8% | 3.9% | (many diffs) |
| 42 | mouse | 14268 | Fn1 | fibronectin 1 | NM_001276409.1 | 79.7% | 3.8% | (many diffs) |
| 43 | mouse | 14268 | Fn1 | fibronectin 1 | NM_001276408.1 | 78.8% | 3.8% | (many diffs) |
| 44 | mouse | 14268 | Fn1 | fibronectin 1 | XM_006495699.3 | 78.8% | 3.8% | (many diffs) |
| 45 | mouse | 14268 | Fn1 | fibronectin 1 | XM_006495697.3 | 76.7% | 3.7% | (many diffs) |
| 46 | mouse | 14268 | Fn1 | fibronectin 1 | NM_010233.2 | 76% | 3.7% | (many diffs) |
| 47 | mouse | 14268 | Fn1 | fibronectin 1 | XM_017315775.1 | 74.1% | 3.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 369
- ORF length:
- 300
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcttaggggt ccggggcccg ggctgctgct gctggccgtc ctgtgcctgg 121 ggacagcggt gccctccacg ggagcctcga agagcaagag gcaggctcag caaatggttc 181 agccccagtc cccggtggct gtcagtcaaa gcaagcccgg ttgttatgac aatggaaaac 241 actatcagat aaatcaacag tgggagcgga cctacctagg caatgcgttg gtttgtactt 301 gttatggagg aagccgaggt tttaactgcg agagtaaacc tgaagctgaa gagacttgct 361 ttgacaagta gtctgggaac acttaccgag tgggtgacac ttatgagcgt cctaaagact 421 ccatgatctg ggactgtacc tgcatcgggg ctgggcgagg gagaataagc tgtaccatcg 481 caaaccgctg ccatgaaggg ggtcagtcct acaagattgg tgacacctgg aggagaccac 541 atgagactgg tggttacatg ttagagtgtg tgtgtcttgg taatggaaaa ggagaatgga 601 cctgcaagcc catagctgag aagtgttttg atcatgctgc tgggacttcc tatgtggtcg 661 gagaaacgtg ggagaagccc taccaaggct ggatgatggt agattgtact tgcctgggag 721 aaggcagcgg acgcatcact tgcacttcta gaaatagatg caacgatcag gacacaagga 781 catcctatag aattggagac acctggagca agaaggataa tcgaggaaac ctgctccagt 841 gcatctgcac aggcaacggc cgaggagagt ggaagtgtga gaggcacacc tctgtgcaga 901 ccacatcgag cggatctggc cccttcaccg atgttcgtgc agctgtttac caaccgcagc 961 ctcaccccca gcctcctccc tatggccact gtgtcacaga cagtggtgtg gtctactctg 1021 tggggatgca gtggctgaag acacaaggaa ataagcaaat gctttgcacg tgcctgggca 1081 acggagtcag ctgccaagag acagctgtaa cccagactta cggtggcaac tcaaatggag 1141 agccatgtgt cttaccattc acctacaatg gcaggacgtt ctactcctgc accacagaag 1201 ggcgacagga cggacatctt tggtgcagca caacttcgaa ttatgagcag gaccagaaat 1261 actctttctg cacagaccac actgttttgg ttcagactcg aggaggaaat tccaatggtg 1321 ccttgtgcca cttccccttc ctatacaaca accacaatta cactgattgc acttctgagg 1381 gcagaagaga caacatgaag tggtgtggga ccacacagaa ctatgatgcc gaccagaagt 1441 ttgggttctg ccccatggct gcccacgagg aaatctgcac aaccaatgaa ggggtcatgt 1501 accgcattgg agatcagtgg gataagcagc atgacatggg tcacatgatg aggtgcacgt 1561 gtgttgggaa tggtcgtggg gaatggacat gcattgccta ctcgcagctt cgagatcagt 1621 gcattgttga tgacatcact tacaatgtga acgacacatt ccacaagcgt catgaagagg 1681 ggcacatgct gaactgtaca tgcttcggtc agggtcgggg caggtggaag tgtgatcccg 1741 tcgaccaatg ccaggattca gagactggga cgttttatca aattggagat tcatgggaga 1801 agtatgtgca tggtgtcaga taccagtgct actgctatgg ccgtggcatt ggggagtggc 1861 attgccaacc tttacagacc tatccaagct caagtggtcc tgtcgaagta tttatcactg 1921 agactccgag tcagcccaac tcccacccca tccagtggaa tgcaccacag ccatctcaca 1981 tttccaagta cattctcagg tggagaccta aaaattctgt aggccgttgg aaggaagcta 2041 ccataccagg ccacttaaac tcctacacca tcaaaggcct gaagcctggt gtggtatacg 2101 agggccagct catcagcatc cagcagtacg gccaccaaga agtgactcgc tttgacttca 2161 ccaccaccag caccagcaca cctgtgacca gcaacaccgt gacaggagag acgactccct 2221 tttctcctct tgtggccact tctgaatctg tgaccgaaat cacagccagt agctttgtgg 2281 tctcctgggt ctcagcttcc gacaccgtgt cgggattccg ggtggaatat gagctgagtg 2341 aggagggaga tgagccacag tacctggatc ttccaagcac agccacttct gtgaacatcc 2401 ctgacctgct tcctggccga aaatacattg taaatgtcta tcagatatct gaggatgggg 2461 agcagagttt gatcctgtct acttcacaaa caacagcgcc tgatgcccct cctgacccga 2521 ctgtggacca agttgatgac acctcaattg ttgttcgctg gagcagaccc caggctccca 2581 tcacagggta cagaatagtc tattcgccat cagtagaagg tagcagcaca gaactcaacc 2641 ttcctgaaac tgcaaactcc gtcaccctca gtgacttgca acctggtgtt cagtataaca 2701 tcactatcta tgctgtggaa gaaaatcaag aaagtacacc tgttgtcatt caacaagaaa 2761 ccactggcac cccacgctca gatacagtgc cctctcccag ggacctgcag tttgtggaag 2821 tgacagacgt gaaggtcacc atcatgtgga caccgcctga gagtgcagtg accggctacc 2881 gtgtggatgt gatccccgtc aacctgcctg gcgagcacgg gcagaggctg cccatcagca 2941 ggaacacctt tgcagaagtc accgggctgt cccctggggt cacctattac ttcaaagtct 3001 ttgcagtgag ccatgggagg gagagcaagc ctctgactgc tcaacagaca accaaactgg 3061 atgctcccac taacctccag tttgtcaatg aaactgattc tactgtcctg gtgagatgga 3121 ctccacctcg ggcccagata acaggatacc gactgaccgt gggccttacc cgaagaggcc 3181 agcccaggca gtacaatgtg ggtccctctg tctccaagta ccccctgagg aatctgcagc 3241 ctgcatctga gtacaccgta tccctcgtgg ccataaaggg caaccaagag agccccaaag 3301 ccactggagt ctttaccaca ctgcagcctg ggagctctat tccaccttac aacaccgagg 3361 tgactgagac caccattgtg atcacatgga cgcctgctcc aagaattggt tttaagctgg 3421 gtgtacgacc aagccaggga ggagaggcac cacgagaagt gacttcagac tcaggaagca 3481 tcgttgtgtc cggcttgact ccaggagtag aatacgtcta caccatccaa gtcctgagag 3541 atggacagga aagagatgcg ccaattgtaa acaaagtggt gacaccattg tctccaccaa 3601 caaacttgca tctggaggca aaccctgaca ctggagtgct cacagtctcc tgggagagga 3661 gcaccacccc agacattact ggttatagaa ttaccacaac ccctacaaac ggccagcagg 3721 gaaattcttt ggaagaagtg gtccatgctg atcagagctc ctgcactttt gataacctga 3781 gtcccggcct ggagtacaat gtcagtgttt acactgtcaa ggatgacaag gaaagtgtcc 3841 ctatctctga taccatcatc ccagctgttc ctcctcccac tgacctgcga ttcaccaaca 3901 ttggtccaga caccatgcgt gtcacctggg ctccaccccc atccattgat ttaaccaact 3961 tcctggtgcg ttactcacct gtgaaaaatg aggaagatgt tgcagagttg tcaatttctc 4021 cttcagacaa tgcagtggtc ttaacaaatc tcctgcctgg tacagaatat gtagtgagtg 4081 tctccagtgt ctacgaacaa catgagagca cacctcttag aggaagacag aaaacaggtc 4141 ttgattcccc aactggcatt gacttttctg atattactgc caactctttt actgtgcact 4201 ggattgctcc tcgagccacc atcactggct acaggatccg ccatcatccc gagcacttca 4261 gtgggagacc tcgagaagat cgggtgcccc actctcggaa ttccatcacc ctcaccaacc 4321 tcactccagg cacagagtat gtggtcagca tcgttgctct taatggcaga gaggaaagtc 4381 ccttattgat tggccaacaa tcaacagttt ctgatgttcc gagggacctg gaagttgttg 4441 ctgcgacccc caccagccta ctgatcagct gggatgctcc tgctgtcaca gtgagatatt 4501 acaggatcac ttacggagaa acaggaggaa atagccctgt ccaggagttc actgtgcctg 4561 ggagcaagtc tacagctacc atcagcggcc ttaaacctgg agttgattat accatcactg 4621 tgtatgctgt cactggccgt ggagacagcc ccgcaagcag caagccaatt tccattaatt 4681 accgaacaga aattgacaaa ccatcccaga tgcaagtgac cgatgttcag gacaacagca 4741 ttagtgtcaa gtggctgcct tcaagttccc ctgttactgg ttacagagta accaccactc 4801 ccaaaaatgg accaggacca acaaaaacta aaactgcagg tccagatcaa acagaaatga 4861 ctattgaagg cttgcagccc acagtggagt atgtggttag tgtctatgct cagaatccaa 4921 gcggagagag tcagcctctg gttcagactg cagtaaccac tattcctgca ccaactgacc 4981 tgaagttcac tcaggtcaca cccacaagcc tgagcgccca gtggacacca cccaatgttc 5041 agctcactgg atatcgagtg cgggtgaccc ccaaggagaa gaccggacca atgaaagaaa 5101 tcaaccttgc tcctgacagc tcatccgtgg ttgtatcagg acttatggtg gccaccaaat 5161 atgaagtgag tgtctatgct cttaaggaca ctttgacaag cagaccagct cagggtgttg 5221 tcaccactct ggagaatgtc agcccaccaa gaagggctcg tgtgacagat gctactgaga 5281 ccaccatcac cattagctgg agaaccaaga ctgagacgat cactggcttc caagttgatg 5341 ccgttccagc caatggccag actccaatcc agagaaccat caagccagat gtcagaagct 5401 acaccatcac aggtttacaa ccaggcactg actacaagat ctacctgtac accttgaatg 5461 acaatgctcg gagctcccct gtggtcatcg acgcctccac tgccattgat gcaccatcca 5521 acctgcgttt cctggccacc acacccaatt ccttgctggt atcatggcag ccgccacgtg 5581 ccaggattac cggctacatc atcaagtatg agaagcctgg gtctcctccc agagaagtgg 5641 tccctcggcc ccgccctggt gtcacagagg ctactattac tggcctggaa ccgggaaccg 5701 aatatacaat ttatgtcatt gccctgaaga ataatcagaa gagcgagccc ctgattggaa 5761 ggaaaaagac aggacaagaa gctctctctc agacaaccat ctcatgggcc ccattccagg 5821 acacttctga gtacatcatt tctagtcatc ctgttggcac tgatgaagaa cccttacagt 5881 tcagggttcc tggaacttct accagtgcca ctctgacagg cctcaccaga ggtgccacct 5941 acaacatcat agtggaggca ctgaaagacc agcagaggca taaggttcgg gaagaggttg 6001 ttaccgtggg caactctgtc aacgaaggct tgaaccaacc tacggatgac tcgtgctttg 6061 acccctacac agtttcccat tatgccgttg gagatgagtg ggaacgaatg tctgaatcag 6121 gctttaaact gttgtgccag tgcttaggct ttggaagtgg tcatttcaga tgtgattcat 6181 ctagatggtg ccatgacaat ggtgtgaact acaagattGG AGAGAAGTGG GACCGTCAGG 6241 GAGAAAATGG CCAGATGATG AGCTGCACAT GTCTTGGGAA CGGAAAAGGA GAATTCAAGT 6301 GTGACCCTCA TGAGGCAACG TGTTATGATG ATGGGAAGAC ATACCACGTA GGAGAACAGT 6361 GGCAGAAGGA ATATCTCGGT GCCATTTGCT CCTGCACATG CTTTGGAGGC CAGCGGGGCT 6421 GGCGCTGTGA CAACTGCCGC AGACCTGGGG GTGAACCCAG TCCCGAAGGC ACTACTGGCC 6481 AGTCCTACAA CCAGTATTCT CAGAGATACC ATCAGAGAAC AAACACTAAT GTTAATTGCC 6541 CAATTGAGTG CTTCATGCCT TTAGATGTAC AGGCTGACAG AGAAGATTCC CGAGAGTTGC 6601 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 6661 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 6721 ATATATCTTG TGGAAAGGAC GAACGCAATG CTAACAACCA AAGATGACGC GTTAAGTCga 6781 caatcaacct ctggattaca aaatttgtga aagatt