Transcript: Mouse XM_017315775.1

PREDICTED: Mus musculus fibronectin 1 (Fn1), transcript variant X2, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Fn1 (14268)
Length:
8209
CDS:
248..7516

Additional Resources:

NCBI RefSeq record:
XM_017315775.1
NBCI Gene record:
Fn1 (14268)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_017315775.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000306639 TGATGTCCGAACAGCTATTTA pLKO_005 1108 CDS 100% 15.000 21.000 N Fn1 n/a
2 TRCN0000090370 GCTAAACTCTTCCACCATTAT pLKO.1 4111 CDS 100% 13.200 18.480 N Fn1 n/a
3 TRCN0000090369 GCCAGGATTACTGGCTACATT pLKO.1 6299 CDS 100% 0.563 0.788 N Fn1 n/a
4 TRCN0000090371 CCTGTCAACAGAAATACCTTT pLKO.1 3107 CDS 100% 4.950 3.960 N Fn1 n/a
5 TRCN0000327176 CCTGTCAACAGAAATACCTTT pLKO_005 3107 CDS 100% 4.950 3.960 N Fn1 n/a
6 TRCN0000090372 GCCAGTTTCCATCAATTATAA pLKO.1 5113 CDS 100% 15.000 10.500 N Fn1 n/a
7 TRCN0000354173 GCCAGTTTCCATCAATTATAA pLKO_005 5113 CDS 100% 15.000 10.500 N Fn1 n/a
8 TRCN0000306574 TGCCGTCTCTGTGCGCTATTA pLKO_005 4930 CDS 100% 13.200 9.240 N Fn1 n/a
9 TRCN0000090368 GCCTAGAAATACCTTTCTCTT pLKO.1 8000 3UTR 100% 0.495 0.347 N Fn1 n/a
10 TRCN0000327177 GCCTAGAAATACCTTTCTCTT pLKO_005 8000 3UTR 100% 0.495 0.347 N Fn1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017315775.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000471752 ACGCAATGCTAACAACCAAAGATG pLX_317 5.9% 74.1% 3.7% V5 (not translated due to prior stop codon) (many diffs) n/a
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