Construct: ORF TRCN0000471769
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013399.1_s317c1
- Derived from:
- ccsbBroadEn_12288
- DNA Barcode:
- ATGACCTACGCTCGGTAGGCACAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ACSS2 (55902)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471769
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55902 | ACSS2 | acyl-CoA synthetase short c... | NM_001242393.1 | 72.9% | 72.9% | 1_492del |
| 2 | human | 55902 | ACSS2 | acyl-CoA synthetase short c... | XM_006723826.1 | 70.2% | 70.2% | 1_492del;547_585del;1302_1331del |
| 3 | human | 55902 | ACSS2 | acyl-CoA synthetase short c... | XM_011528912.2 | 68.8% | 68.8% | 1_531del;586_624del;1341_1370del |
| 4 | human | 55902 | ACSS2 | acyl-CoA synthetase short c... | XM_011528911.1 | 65.5% | 65.5% | 1_627del;682_720del;1437_1466del |
| 5 | human | 55902 | ACSS2 | acyl-CoA synthetase short c... | XM_011528909.2 | 64.3% | 64.3% | 1_666del;721_759del;1476_1505del |
| 6 | human | 55902 | ACSS2 | acyl-CoA synthetase short c... | NM_018677.4 | 63% | 63% | 1_777del |
| 7 | human | 55902 | ACSS2 | acyl-CoA synthetase short c... | XM_005260456.2 | 62.1% | 62.1% | 1_777del;1548_1577del |
| 8 | human | 55902 | ACSS2 | acyl-CoA synthetase short c... | NM_001076552.2 | 61.9% | 61.9% | 1_777del;832_870del |
| 9 | human | 55902 | ACSS2 | acyl-CoA synthetase short c... | XM_011528908.1 | 61.9% | 61.9% | 1_816del |
| 10 | human | 55902 | ACSS2 | acyl-CoA synthetase short c... | XM_005260455.2 | 61% | 61% | 1_777del;832_870del;1587_1616del |
| 11 | human | 55902 | ACSS2 | acyl-CoA synthetase short c... | XM_011528907.1 | 61% | 61% | 1_816del;1587_1616del |
| 12 | human | 55902 | ACSS2 | acyl-CoA synthetase short c... | XM_011528906.1 | 60.7% | 60.7% | 1_816del;871_909del |
| 13 | human | 55902 | ACSS2 | acyl-CoA synthetase short c... | XM_011528905.1 | 59.9% | 59.9% | 1_816del;871_909del;1626_1655del |
| 14 | mouse | 60525 | Acss2 | acyl-CoA synthetase short-c... | XM_006499975.1 | 73.6% | 76.6% | (many diffs) |
| 15 | mouse | 60525 | Acss2 | acyl-CoA synthetase short-c... | XM_006499974.1 | 65.1% | 67.8% | (many diffs) |
| 16 | mouse | 60525 | Acss2 | acyl-CoA synthetase short-c... | NM_019811.3 | 57.5% | 59.9% | (many diffs) |
| 17 | mouse | 60525 | Acss2 | acyl-CoA synthetase short-c... | XM_006499973.1 | 53.2% | 55.4% | (many diffs) |
| 18 | mouse | 60525 | Acss2 | acyl-CoA synthetase short-c... | XM_017319156.1 | 46.5% | 48.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1392
- ORF length:
- 1326
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg tgactccacc agccagtccc ccccaattaa gaggtcatgc ccagatgtgc 121 agatctcatg gaaccaaggg attgacttgt ggtggcatga gctcatgcaa gaggcagggg 181 atgagtgtga gcccgagtgg tgtgatgccg aggacccact cttcatcctg tacaccagtg 241 gctccacagg caaacccaag ggtgtggttc acacagttgg gggctacatg ctctatgtag 301 ccacaacctt caagtatgtg tttgacttcc atgcagagga tgtgttctgg tgcacggcag 361 acattggttg gatcactggt cattcctacg tcacctatgg gccactggcc aatggtgcca 421 ccagtgtttt gtttgagggg attcccacat atccggacgt gaaccgcctg tggagcattg 481 tggacaaata caaggtgacc aagttctaca cagcacccac agccatccgt ctgctcatga 541 agtttggaga tgagcctgtc accaagcata gccgggcatc cttgcaggtg ttaggcacag 601 tgggtgaacc catcaaccct gaggcctggc tatggtacca ccgggtggta ggtgcccagc 661 gctgccccat cgtggacacc ttctggcaaa cagagacagg tggccacatg ttgactcccc 721 ttcctggtgc cacacccatg aaacccggtt ctgctacttt cccattcttt ggtgtagctc 781 ctgcaatcct gaatgagtcc ggggaagagt tggaaggtga agctgaaggt tatctggtgt 841 tcaagcagcc ctggccaggg atcatgcgca cagtctatgg gaaccacgaa cgctttgaga 901 caacctactt taagaagttt cctggatact atgttacagg AGATGGCTGC CAGCGGGACC 961 AGGATGGCTA TTACTGGATC ACTGGCAGGA TTGATGACAT GCTCAATGTA TCTGGACACC 1021 TGCTGAGTAC AGCAGAGGTG GAGTCAGCAC TTGTGGAACA TGAGGCTGTT GCAGAGGCAG 1081 CTGTGGTGGG CCACCCTCAT CCTGTGAAGG GTGAATGCCT CTACTGCTTT GTCACCTTGT 1141 GTGATGGCCA CACCTTCAGC CCCAAGCTCA CCGAGGAGCT CAAGAAGCAG ATTAGAGAAA 1201 AGATTGGCCC CATTGCCACA CCAGACTACA TCCAGAATGC ACCTGGCTTG CCTAAAACCC 1261 GCTCAGGGAA AATCATGAGG CGAGTGCTTC GGAAGATTGC TCAGAATGAC CATGACCTCG 1321 GGGACATGTC TACTGTGGCT GACCCATCTG TCATCAGTCA CCTCTTCAGC CACCGCTGCC 1381 TGACCATCCA GTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1441 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1501 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAATG ACCTACGCTC GGTAGGCACA 1561 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t