Construct: ORF TRCN0000471783

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009749.1_s317c1
Derived from:
ccsbBroadEn_00426
DNA Barcode:
TTGCTGGCTACCCGAGCTAGAACG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DCN (1634)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000471783
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1634 DCN decorin NM_001920.5 100% 100%
2 human 1634 DCN decorin NM_133503.3 100% 100%
3 human 1634 DCN decorin NM_133504.3 69.6% 69.6% 210_211ins327
4 human 1634 DCN decorin NM_133505.3 59% 59% 210_211ins441
5 human 1634 DCN decorin NM_133506.3 47.9% 47.9% 324_325ins561
6 human 1634 DCN decorin NM_133507.3 20.7% 20% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1143
ORF length:
1077
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgaa ggccactatc atcctccttc tgcttgcaca agtttcctgg gctggaccgt
121ttcaacagag aggcttattt gactttatgc tagaagatga ggcttctggg ataggcccag
181aagttcctga tgaccgcgac ttcgagccct ccctaggccc agtgtgcccc ttccgctgtc
241aatgccatct tcgagtggtc cagtgttctg atttgggtct ggacaaagtg ccaaaggatc
301ttccccctga cacaactctg ctagacctgc aaaacaacaa aataaccgaa atcaaagatg
361gagactttaa gaacctgaag aaccttcacg cattgattct tgtcaacaat aaaattagca
421aagttagtcc tggagcattt acacctttgg tgaagttgga acgactttat ctgtccaaga
481atcagctgaa ggaattgcca gaaaaaatgc ccaaaactct tcaggagctg cgtgcccatg
541agaatgagat caccaaagtg cgaaaagtta ctttcaatgg actgaaccag atgattgtca
601tagaactggg caccaatCCG CTGAAGAGCT CAGGAATTGA AAATGGGGCT TTCCAGGGAA
661TGAAGAAGCT CTCCTACATC CGCATTGCTG ATACCAATAT CACCAGCATT CCTCAAGGTC
721TTCCTCCTTC CCTTACGGAA TTACATCTTG ATGGCAACAA AATCAGCAGA GTTGATGCAG
781CTAGCCTGAA AGGACTGAAT AATTTGGCTA AGTTGGGATT GAGTTTCAAC AGCATCTCTG
841CTGTTGACAA TGGCTCTCTG GCCAACACGC CTCATCTGAG GGAGCTTCAC TTGGACAACA
901ACAAGCTTAC CAGAGTACCT GGTGGGCTGG CAGAGCATAA GTACATCCAG GTTGTCTACC
961TTCATAACAA CAATATCTCT GTAGTTGGAT CAAGTGACTT CTGCCCACCT GGACACAACA
1021CCAAAAAGGC TTCTTATTCG GGTGTGAGTC TTTTCAGCAA CCCGGTCCAG TACTGGGAGA
1081TACAGCCATC CACCTTCAGA TGTGTCTACG TGCGCTCTGC CATTCAACTC GGAAACTATA
1141AGTATCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC
1201TCGGTCTCGA TTCTACGTAG TAATGAACTA GCCCGTAACT TGAAAGTATT TCGATTTCTT
1261GGCTTTATAT ATCTTGTGGA AAGGACGATT GCTGGCTACC CGAGCTAGAA CGACGCGTTA
1321AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt

Download FASTA (ORF) (Full)