Construct: ORF TRCN0000471784
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007983.1_s317c1
- Derived from:
- ccsbBroadEn_01664
- DNA Barcode:
- ACACGTAACCAGGCTGCGGTGGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TGFA (7039)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471784
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7039 | TGFA | transforming growth factor ... | NM_001099691.3 | 100% | 100% | |
| 2 | human | 7039 | TGFA | transforming growth factor ... | NM_003236.4 | 99.3% | 99.3% | 94_96delGCA |
| 3 | human | 7039 | TGFA | transforming growth factor ... | NM_001308159.2 | 93.7% | 90.9% | (many diffs) |
| 4 | human | 7039 | TGFA | transforming growth factor ... | NM_001308158.2 | 93.2% | 90.3% | (many diffs) |
| 5 | mouse | 21802 | Tgfa | transforming growth factor ... | NM_031199.4 | 88.4% | 92.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 543
- ORF length:
- 477
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttG TACAAAAAAG 61 TTGGCATGGT CCCCTCGGCT GGACAGCTCG CCCTGTTCGC TCTGGGTATT GTGTTGGCTG 121 CGTGCCAGGC CTTGGAGAAC AGCACGTCCC CGCTGAGTGA CCCGCCCGTG GCTGCAGCAG 181 TGGTGTCCCA TTTTAATGAC TGCCCAGATT CCCACACTCA GTTCTGCTTC CATGGAACCT 241 GCAGGTTTTT GGTGCAGGAG GACAAGCCAG CATGTGTCTG CCATTCTGGG TACGTTGGTG 301 CACGCTGTGA GCATGCGGAC CTCCTGGCCG TGGTGGCTGC CAGCCAGAAG AAGCAGGCCA 361 TCACCGCCTT GGTGGTGGTC TCCATCGTGG CCCTGGCTGT CCTTATCATC ACATGTGTGC 421 TGATACACTG CTGCCAGGTC CGAAAACACT GTGAGTGGTG CCGGGCCCTC ATCTGCCGGC 481 ACGAGAAGCC CAGCGCCCTC CTGAAGGGAA GAACCGCTTG CTGCCACTCA GAAACAGTGG 541 TCTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 601 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 661 GGCTTTATAT ATCTTGTGGA AAGGACGAAC ACGTAACCAG GCTGCGGTGG CGACGCGTTA 721 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt