Construct: ORF TRCN0000471787

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012628.3_s317c1
Derived from:
ccsbBroadEn_15140
DNA Barcode:
ACGTGGTTCACAAACTTTGGTTAG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
WNK1 (65125)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000471787
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 65125 WNK1 WNK lysine deficient protei... XM_017019839.1 19% 18.8% (many diffs)
2 human 65125 WNK1 WNK lysine deficient protei... XM_011521009.2 18.5% 18.2% (many diffs)
3 human 65125 WNK1 WNK lysine deficient protei... XM_011521008.2 18.5% 18.2% (many diffs)
4 human 65125 WNK1 WNK lysine deficient protei... NM_014823.3 18.2% 18% (many diffs)
5 human 65125 WNK1 WNK lysine deficient protei... XM_017019838.1 18.2% 18% (many diffs)
6 human 65125 WNK1 WNK lysine deficient protei... XM_017019837.1 18.2% 18% (many diffs)
7 human 65125 WNK1 WNK lysine deficient protei... XM_017019836.1 17.7% 17.4% (many diffs)
8 human 65125 WNK1 WNK lysine deficient protei... XM_017019835.1 17.7% 17.4% (many diffs)
9 human 65125 WNK1 WNK lysine deficient protei... XM_017019834.1 17.5% 17.2% (many diffs)
10 human 65125 WNK1 WNK lysine deficient protei... XM_011521007.2 16.5% 16.3% (many diffs)
11 human 65125 WNK1 WNK lysine deficient protei... XM_011521006.2 16.5% 16.3% (many diffs)
12 human 65125 WNK1 WNK lysine deficient protei... XM_006719003.2 16.3% 16.1% (many diffs)
13 human 65125 WNK1 WNK lysine deficient protei... NM_018979.4 16.3% 16.1% (many diffs)
14 human 65125 WNK1 WNK lysine deficient protei... XM_011521004.2 15.3% 15.1% (many diffs)
15 human 65125 WNK1 WNK lysine deficient protei... XM_011521003.2 15.3% 15% (many diffs)
16 human 65125 WNK1 WNK lysine deficient protei... NM_213655.4 14.8% 14.6% (many diffs)
17 human 65125 WNK1 WNK lysine deficient protei... XM_011521002.2 14.7% 14.5% (many diffs)
18 human 65125 WNK1 WNK lysine deficient protei... NM_001184985.1 14.7% 14.5% (many diffs)
19 human 65125 WNK1 WNK lysine deficient protei... XM_011521001.2 14.4% 14.2% (many diffs)
20 human 65125 WNK1 WNK lysine deficient protei... XM_011521000.2 14.2% 14% (many diffs)
21 human 65125 WNK1 WNK lysine deficient protei... XM_011520998.2 13.9% 13.7% (many diffs)
22 human 65125 WNK1 WNK lysine deficient protei... XM_011520999.2 13.9% 13.7% (many diffs)
23 human 65125 WNK1 WNK lysine deficient protei... XM_011520997.3 13.9% 13.7% (many diffs)
24 mouse 232341 Wnk1 WNK lysine deficient protei... NM_001185020.1 15.8% 16.7% (many diffs)
25 mouse 232341 Wnk1 WNK lysine deficient protei... XM_017321557.1 15.8% 16.7% (many diffs)
26 mouse 232341 Wnk1 WNK lysine deficient protei... XM_017321556.1 15.4% 16.3% (many diffs)
27 mouse 232341 Wnk1 WNK lysine deficient protei... XM_017321555.1 15.4% 16.2% (many diffs)
28 mouse 232341 Wnk1 WNK lysine deficient protei... NM_001185021.1 15.3% 16.1% (many diffs)
29 mouse 232341 Wnk1 WNK lysine deficient protei... XM_017321554.1 15.3% 16.1% (many diffs)
30 mouse 232341 Wnk1 WNK lysine deficient protei... XM_017321553.1 15.1% 15.9% (many diffs)
31 mouse 232341 Wnk1 WNK lysine deficient protei... XM_017321552.1 14.8% 15.6% (many diffs)
32 mouse 232341 Wnk1 WNK lysine deficient protei... XM_017321550.1 14.5% 15.3% (many diffs)
33 mouse 232341 Wnk1 WNK lysine deficient protei... NM_198703.3 14.1% 14.9% (many diffs)
34 mouse 232341 Wnk1 WNK lysine deficient protei... XM_017321549.1 14.1% 14.9% (many diffs)
35 mouse 232341 Wnk1 WNK lysine deficient protei... XM_017321548.1 14.1% 14.8% (many diffs)
36 mouse 232341 Wnk1 WNK lysine deficient protei... XM_017321547.1 14% 14.8% (many diffs)
37 mouse 232341 Wnk1 WNK lysine deficient protei... XM_017321546.1 14% 14.7% (many diffs)
38 mouse 232341 Wnk1 WNK lysine deficient protei... XM_017321545.1 13.9% 14.7% (many diffs)
39 mouse 232341 Wnk1 WNK lysine deficient protei... XM_017321544.1 13.9% 14.6% (many diffs)
40 mouse 232341 Wnk1 WNK lysine deficient protei... XM_017321543.1 13.9% 14.6% (many diffs)
41 mouse 232341 Wnk1 WNK lysine deficient protei... XM_011241320.2 13.8% 14.6% (many diffs)
42 mouse 232341 Wnk1 WNK lysine deficient protei... XM_017321542.1 13.8% 14.6% (many diffs)
43 mouse 232341 Wnk1 WNK lysine deficient protei... NM_001199083.1 12.8% 13.5% (many diffs)
44 mouse 232341 Wnk1 WNK lysine deficient protei... NM_001199084.1 12.8% 13.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1245
ORF length:
1179
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtc tggcggcgcc gcagagaagc agagcagcac tcccggttcc ctgttcctct
121cgccgccggc tcctgccccc aagaatggct ccagctccga ttcctccgtg ggggagaaac
181tgggagccgc ggccgccgac gctgtgaccg gcaggaccga ggagtacagg cgccgccgcc
241acactatgga caaggacagc cgtggggcgg ccgcgaccac taccaccact gagcaccgct
301tcttccgccg gagcgtcatc tgcgactcca atgccactgc actggagctt cccggccttc
361ctctttccct gccccagccc agcatccccg cggctgtccc gcagagtgct ccaccggagc
421cccaccggga agagaccgtg accgccaccg ccacttccca ggtagcccag cagcctccag
481ccgctgccgc ccctggggaa caggccgtcg cgggccctgc cccctcgact gtccccagca
541gtaccagcaa agaccgccca gtgtcccagc ctagccttgt ggggagcaaa gaggagccgc
601cgccggcgag aagtggcagc ggcggcggca gcgccaagga gccacaggag gaacggagcc
661agcagcagga tgatatcgaa gagctggaga ccaaggccgt gggaatgtct aacgatggcc
721gctttctcaa gtttgacatc gaaatcggca gaggctcctt taagacggtc tacaaaggtc
781tggacactga aaccaccgtg gaagtcgcct ggtgtgaact gcaggatcga aaattaacaa
841agtctgagag gcagagattt aaagaagaag ctgaaatgtt aaaaggTCTT CAGCATCCCA
901ATATTGTTAG ATTTTATGAT TCCTGGGAAT CCACAGTAAA AGGAAAGAAG TGCATTGTTT
961TGGTGACTGA ACTTATGACG TCTGGAACAC TTAAAACGTA TCTGAAAAGG TTTAAAGTGA
1021TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGTCTT CAGTTTCTTC
1081ATACTCGAAC TCCACCTATC ATTCACCGCG ATCTTAAATG TGACAACATC TTTATCACCG
1141GCCCTACTGG CTCAGTCAAG ATTGGAGACC TCGGTCTGGC AACCCTGAAG CGGGCTTCTT
1201TTGCCAAGAG TGTGATAGGT ATGTTTCAGG TGTACCTTGG AGCCTGCCCA ACTTTCTTGT
1261ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT
1321AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG
1381GAAAGGACGA ACGTGGTTCA CAAACTTTGG TTAGACGCGT TAAGTCgaca atcaacctct
1441ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)