Construct: ORF TRCN0000471791
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017881.1_s317c1
- Derived from:
- ccsbBroadEn_12999
- DNA Barcode:
- TCACGCAAGCCACTCCTGCTGTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TADA2B (93624)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471791
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 93624 | TADA2B | transcriptional adaptor 2B | XM_011513595.1 | 100% | 100% | |
| 2 | human | 93624 | TADA2B | transcriptional adaptor 2B | NM_152293.3 | 78% | 78% | 1_276del |
| 3 | mouse | 231151 | Tada2b | transcriptional adaptor 2B | XM_030254386.1 | 83.7% | 95.5% | (many diffs) |
| 4 | mouse | 231151 | Tada2b | transcriptional adaptor 2B | NM_001170454.1 | 67.7% | 77.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1053
- ORF length:
- 984
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggctgcccac gttggtgctt cccggactcc ccaagaggtg atggagcatt 121 acgtgagcat gtacatccac gggaacctgg ggaaggcctg catccccgac accatcccca 181 accgcgtgac agaccacacc tgtcccagcg gaggccccct ctcacccagc ctcaccaccc 241 cgctgccccc gctggacatc tctgtggctg agcagcagca gctgggctac atgccgctgc 301 gggatgatta cgagatcgag tatgaccagg atgccgagac gctcatcagc gggctctctg 361 tcaactatga tgacgacgac gtggagatcg agctgaagcg cgcccacgtg gacatgtacg 421 tgcggaagct gaaagagaga cagcggcgga agaacatcgc ccgtgactac aatctggtgc 481 cagccttcct ggggaaggac aagaaggaga aggaaaaggc gctgaagcgc aagatcacca 541 aggaggagaa ggagctgcgc ctgaagctga ggccgctgta ccagttcatg tcatgcaagg 601 agtttgatga cctttttgaa aacatgcaca aagaaaaaat gctccgggcc aagatccgag 661 aactgcagcg gtaccggcga aacgggatCA CCAAGATGGA AGAGTCGGCA GAGTACGAGG 721 CAGCGCGGCA TAAACGGGAG AAGAGGAAGG AGAACAAAAA CCTAGCCGGC TCCAAACGGG 781 GAAAGGAGGA CGGCAAAGAC AGCGAGTTCG CCGCCATTGA GAACCTTCCA GGCTTCGAGC 841 TCCTGTCAGA TCGCGAGAAG GTGCTCTGCA GCTCTTTAAA CTTGAGTCCA GCCCGCTACG 901 TGACTGTGAA GACTATTATA ATTAAAGACC ACCTCCAGAA GCGGCAAGGA ATCCCCTCCA 961 AAAGCCGCCT TCCTAGCTAC CTGGACAAAG TCCTAAAGAA AAGGATTTTG AATTTCCTCA 1021 CAGAAAGCGG CTGGATCTCC AGGGACGCGT CTTTGCCAAC TTTCTTGTAC AAAGTGGTTG 1081 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1141 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATC 1201 ACGCAAGCCA CTCCTGCTGT TCACGCGTTA AGTCgacaat caacctctgg attacaaaat 1261 ttgtgaaaga tt