Construct: ORF TRCN0000471797
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004335.1_s317c1
- Derived from:
- ccsbBroadEn_09060
- DNA Barcode:
- TAATTTCTTGGTTCTCAACCTTCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- OR51E2 (81285)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471797
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 81285 | OR51E2 | olfactory receptor family 5... | NM_030774.4 | 99.8% | 100% | 777G>T |
| 2 | mouse | 170639 | Olfr78 | olfactory receptor 78 | NM_001168503.1 | 86.8% | 92.8% | (many diffs) |
| 3 | mouse | 170639 | Olfr78 | olfactory receptor 78 | NM_130866.4 | 86.8% | 92.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1026
- ORF length:
- 960
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag ttcctgcaac ttcacacatg ccacctttgt gcttattggt atcccaggat 121 tagagaaagc ccatttctgg gttggcttcc ccctcctttc catgtatgta gtggcaatgt 181 ttggaaactg catcgtggtc ttcatcgtaa ggacggaacg cagcctgcac gctccgatgt 241 acctctttct ctgcatgctt gcagccattg acctggcctt atccacatcc accatgccta 301 agatccttgc ccttttctgg tttgattccc gagagattag ctttgaggcc tgtcttaccc 361 agatgttctt tattcatgcc ctctcagcca ttgaatccac catcctgctg gccatggcct 421 ttgaccgtta tgtggccatc tgccacccac tgcgccatgc tgcagtgctc aacaatacag 481 taacagccca gattggcatc gtggctgtgg tccgcggatc cctctttttt ttcccactgc 541 ctctgctgat caagcggctg gccttctgcc actccaatgt cctctcgcac tccTATTGTG 601 TCCACCAGGA TGTAATGAAG TTGGCCTATG CAGACACTTT GCCCAATGTG GTATATGGTC 661 TTACTGCCAT TCTGCTGGTC ATGGGCGTGG ACGTAATGTT CATCTCCTTG TCCTATTTTC 721 TGATAATACG AACGGTTCTG CAACTGCCTT CCAAGTCAGA GCGGGCCAAG GCCTTTGGAA 781 CCTGTGTGTC ACACATTGGT GTGGTACTCG CCTTCTATGT GCCACTTATT GGCCTCTCAG 841 TTGTACACCG CTTTGGAAAC AGCCTTCATC CCATTGTGCG TGTTGTCATG GGTGACATCT 901 ACCTGCTGCT GCCTCCTGTC ATCAATCCCA TCATCTATGG TGCCAAAACC AAACAGATCA 961 GAACACGGGT GCTGGCTATG TTCAAGATCA GCTGTGACAA GGACTTGCAG GCTGTGGGAG 1021 GCAAGTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1081 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1141 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATAATTTCTT GGTTCTCAAC CTTCGACGCG 1201 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt