Construct: ORF TRCN0000471813

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009499.1_s317c1
Derived from:
ccsbBroadEn_06239
DNA Barcode:
GCGGTCAAACGATAACCCGCAGTC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GABRA3 (2556)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000471813
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2556 GABRA3 gamma-aminobutyric acid typ... NM_000808.4 99.9% 99.7% 539T>C
2 human 2556 GABRA3 gamma-aminobutyric acid typ... XM_006724811.3 69% 62.3% 539T>C;930_931ins212;1020_1021ins244
3 mouse 14396 Gabra3 gamma-aminobutyric acid (GA... NM_008067.4 90.6% 95.7% (many diffs)
4 mouse 14396 Gabra3 gamma-aminobutyric acid (GA... XM_006527823.3 90.6% 95.7% (many diffs)
5 mouse 14396 Gabra3 gamma-aminobutyric acid (GA... XM_011247522.2 90.6% 95.7% (many diffs)
6 mouse 14396 Gabra3 gamma-aminobutyric acid (GA... XM_017318383.1 85.3% 90% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1542
ORF length:
1476
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgat aatcacacaa acaagtcact gttacatgac cagccttggg attcttttcc
121tgattaatat tctccctgga accactggtc aaggggaatc aagacgacaa gaacccgggg
181actttgtgaa gcaggacatt ggcgggctgt ctcctaagca tgccccagat attcctgatg
241acagcactga caacatcact atcttcacca gaatcttgga tcgtcttctg gacggctatg
301acaaccggct gcgacctggg cttggagatg cagtgactga agtgaagact gacatctacg
361tgaccagttt tggccctgtg tcagacactg acatggagta cactattgat gtattttttc
421ggcagacatg gcatgatgaa agactgaaat ttgatggccc catgaagatc cttccactga
481acaatctcct ggctagtaag atctggacac cggacacctt cttccacaat ggcaagaaat
541cagtggctca taacatgacc acgcccaaca agctgctcag attggtggac aacggaaccc
601tcccctatac aatgaggtta acaattcatg ctgagtgtcc catgcatttg gaagattttc
661ccatggatgt gcatgcctgc ccactgaagt ttggaagcta tgcctataca acagctgaag
721tggtttattc ttggactctc ggaaagaaca aatccgtgga agtggcacag gatggttctc
781gcttgaacca gtatgacctt ttgggccatg ttgttgggac agagataatc cggtctagta
841caggagaata tgtcgtcatg acaacccact tccatctcaa gcgaaaaatt ggctactttg
901tgatccagac ctacttgcca tgtatcatga ctgtcattct gtcacaagtg tcgttctggc
961tcaacagaga gtctgttcct gcccgtacag tctttggtgt caccactgtg cttaccatga
1021ccaccttgag tatcagtgcc agaaattcct tacctaaagt ggcatatgcg acggccatgg
1081actggttcat agccgtctgt tatgcctttg tattttctgc actgattgaa tttgccactg
1141tcaactattt caccaagcgg agttgggctt gggaaggcaa gaaggtgcca gaggccctgg
1201agatGAAGAA GAAAACACCA GCAGCCCCAG CAAAGAAAAC CAGCACTACC TTCAACATCG
1261TGGGGACCAC CTATCCCATC AACCTGGCCA AGGACACTGA ATTTTCCACC ATCTCCAAGG
1321GCGCTGCTCC CAGTGCCTCC TCAACCCCAA CAATCATTGC TTCACCCAAG GCCACCTACG
1381TGCAGGACAG CCCGACTGAG ACCAAGACCT ACAACAGTGT CAGCAAGGTT GACAAAATTT
1441CCCGCATCAT CTTTCCTGTG CTCTTTGCCA TATTCAATCT GGTCTATTGG GCCACATATG
1501TCAACCGGGA GTCAGCTATC AAGGGCATGA TCCGCAAACA GTACCCAACT TTCTTGTACA
1561AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT
1621AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA
1681AGGACGAGCG GTCAAACGAT AACCCGCAGT CACGCGTTAA GTCgacaatc aacctctgga
1741ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)