Construct: ORF TRCN0000471819
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016222.1_s317c1
- Derived from:
- ccsbBroadEn_09909
- DNA Barcode:
- GAACAACTGAGTGAAAACAACTCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TCTEX1D2 (255758)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471819
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 255758 | TCTEX1D2 | Tctex1 domain containing 2 | NM_152773.5 | 99.7% | 99.2% | 164A>G |
| 2 | human | 255758 | TCTEX1D2 | Tctex1 domain containing 2 | NM_001351628.2 | 93.5% | 86% | 164A>G;381_382insGACAG;422_444del |
| 3 | mouse | 66061 | Tctex1d2 | Tctex1 domain containing 2 | NM_025329.3 | 83.8% | 83.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 492
- ORF length:
- 426
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cacgtccatc ggagtgtcct tctcggtggg cgacggggtg ccTGAGGCTG 121 AGAAGAACGC AGGGGAGCCC GAGAACACCT ATATTCTGCG GCCTGTTTTC CAGCAGAGGT 181 TCAGGCCCTC TGTGGTTAAA GACTGTATCC ATGCTGTGCT CAAGGAGGGA CTGGCAAATG 241 CTGAATATTC TCCAGAAGAA ATGCCTCAGC TTACAAAACA TTTATCAGAA AACATTAAAG 301 ATAAATTAAA AGAAATGGGA TTTGACCGAT ACAAAATGGT GGTGCAAGTA GTGATTGGAG 361 AACAAAGAGG TGAAGGAGTA TTCATGGCTT CTCGCTGTTT CTGGGATGCT GACACTGACA 421 ACTATACTCA TGATGTTTTC ATGAATGACA GTTTATTCTG CGTTGTAGCA GCATTTGGCT 481 GTTTCTACTA CTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 541 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 601 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGAA CAACTGAGTG AAAACAACTC 661 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t