Construct: ORF TRCN0000471834

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012890.1_s317c1
Derived from:
ccsbBroadEn_11364
DNA Barcode:
GTATGAATAACCAATTCTACAACC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
HS6ST1 (9394)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000471834
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9394 HS6ST1 heparan sulfate 6-O-sulfotr... NM_004807.3 97.4% 97.5% 1_30del;177A>G
2 mouse 50785 Hs6st1 heparan sulfate 6-O-sulfotr... NM_015818.2 88.6% 94.8% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1272
ORF length:
1203
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggttgagcgc gccagcaagt tcgtgctggt ggtggcgggc tcggtgtgct
121tcatgctcat cttgtaccag tacgcgggcc caggactgag cctgggcgcg cccggcggcc
181gcgcgccgcc cgacgacctg gacctgttcc ccacgcccga cccccactac gagaagaagt
241actacttccc ggtccgcgag ctggagcgct cgctgcgctt cgacatgaag ggcgacgacg
301tgatcgtctt cctgcacatc cagaagacgg gcggcaccac cttcggccgc cacctcgtgc
361agaacgtacg cctcgaggtg ccgtgcgact gccggcccgg ccagaagaag tgcacctgct
421accggcccaa ccgccgcgag acttggctct tctcccgctt ctccaccggc tggagctgcg
481ggctgcacgc cgactggacc gagctcacca actgcgtgcc cggcgtgctg gaccgccgcg
541actccgccgc gctgcgcacg cccaggaagt tctactacat caccctgcta cgagaccccg
601tgtcccgcta cctgagcgag tggcggcatg tgcagagggg tgccacgtgg aagacgtcgt
661tgcatatgtg tgatgggcgc acgcccacgc ctgaggagct gccgccctgc tacgagggca
721cggactggtc gggctgcacg ctacaggagt tcatggactg cccgtacaac ctggccaaca
781accgccaggt gcgcatgctg gccgacctga gcctggtggg ctgctacaac ctgtccttca
841tccccgaggg caagcgggcc cagctgctgc tcgagagcgc caagaagaac cTGCGGGGCA
901TGGCCTTCTT CGGCCTGACC GAGTTCCAGC GCAAGACGCA GTACCTGTTC GAGCGGACGT
961TCAACCTCAA GTTCATCCGG CCCTTCATGC AGTACAATAG CACGCGGGCG GGCGGCGTGG
1021AGGTGGATGA AGACACCATC CGGCGCATCG AGGAGCTCAA CGACCTGGAC ATGCAGCTGT
1081ACGACTACGC CAAGGACCTC TTCCAGCAGC GCTACCAGTA CAAGCGGCAG CTGGAGCGCA
1141GGGAGCAGCG CCTGAGGAGC CGCGAGGAGC GTCTGCTGCA CCGGGCCAAG GAGGCACTGC
1201CGCGGGAGGA TGCCGACGAG CCGGGCCGCG TGCCCACCGA GGACTACATG AGCCACATCA
1261TTGAGAAGTG GTTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA
1321ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT
1381CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGTA TGAATAACCA ATTCTACAAC
1441CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)