Construct: ORF TRCN0000471883

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001263.1_s317c1
Derived from:
ccsbBroadEn_00904
DNA Barcode:
GCACTGAAAAAAATGCTCCCACAG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
KCNQ2 (3785)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000471883
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3785 KCNQ2 potassium voltage-gated cha... NM_172109.3 100% 100%
2 human 3785 KCNQ2 potassium voltage-gated cha... NM_172108.5 45.4% 44.8% (many diffs)
3 human 3785 KCNQ2 potassium voltage-gated cha... NM_004518.6 45.3% 44.5% (many diffs)
4 human 3785 KCNQ2 potassium voltage-gated cha... XM_017027844.2 45.1% 44.2% (many diffs)
5 human 3785 KCNQ2 potassium voltage-gated cha... NM_172106.3 45% 44.2% (many diffs)
6 human 3785 KCNQ2 potassium voltage-gated cha... XM_017027842.2 44.3% 43.4% (many diffs)
7 human 3785 KCNQ2 potassium voltage-gated cha... NM_172107.4 43.8% 43.2% (many diffs)
8 human 3785 KCNQ2 potassium voltage-gated cha... XM_011528811.2 43.5% 42.7% (many diffs)
9 human 3785 KCNQ2 potassium voltage-gated cha... XM_017027841.2 43.3% 42.4% (many diffs)
10 human 3785 KCNQ2 potassium voltage-gated cha... XM_011528810.2 43.2% 42.4% (many diffs)
11 human 3785 KCNQ2 potassium voltage-gated cha... XM_017027843.1 36.8% 32.2% (many diffs)
12 mouse 16536 Kcnq2 potassium voltage-gated cha... NM_001006674.2 59.9% 65.3% (many diffs)
13 mouse 16536 Kcnq2 potassium voltage-gated cha... XM_017315768.1 54.8% 60.2% (many diffs)
14 mouse 16536 Kcnq2 potassium voltage-gated cha... NM_001006669.2 47.3% 51.5% (many diffs)
15 mouse 16536 Kcnq2 potassium voltage-gated cha... NM_001003825.3 45.3% 49.4% (many diffs)
16 mouse 16536 Kcnq2 potassium voltage-gated cha... NM_001003824.2 45% 49.1% (many diffs)
17 mouse 16536 Kcnq2 potassium voltage-gated cha... NM_001006668.2 45% 49.9% (many diffs)
18 mouse 16536 Kcnq2 potassium voltage-gated cha... XM_006500567.1 40.6% 44.3% (many diffs)
19 mouse 16536 Kcnq2 potassium voltage-gated cha... XM_006500568.1 40.6% 44.6% (many diffs)
20 mouse 16536 Kcnq2 potassium voltage-gated cha... XM_017315763.1 40.6% 44.5% (many diffs)
21 mouse 16536 Kcnq2 potassium voltage-gated cha... XM_017315767.1 40.5% 44.9% (many diffs)
22 mouse 16536 Kcnq2 potassium voltage-gated cha... NM_001302888.1 40.1% 44% (many diffs)
23 mouse 16536 Kcnq2 potassium voltage-gated cha... XM_006500565.1 40% 43.9% (many diffs)
24 mouse 16536 Kcnq2 potassium voltage-gated cha... XM_017315753.1 39.2% 43.1% (many diffs)
25 mouse 16536 Kcnq2 potassium voltage-gated cha... XM_017315751.1 39.2% 42.7% (many diffs)
26 mouse 16536 Kcnq2 potassium voltage-gated cha... NM_010611.3 39.2% 43% (many diffs)
27 mouse 16536 Kcnq2 potassium voltage-gated cha... XM_017315747.1 39% 42.5% (many diffs)
28 mouse 16536 Kcnq2 potassium voltage-gated cha... XM_017315754.1 38.9% 43.1% (many diffs)
29 mouse 16536 Kcnq2 potassium voltage-gated cha... XM_017315746.1 37.8% 41.5% (many diffs)
30 mouse 16536 Kcnq2 potassium voltage-gated cha... XM_017315743.1 37.6% 41% (many diffs)
31 mouse 16536 Kcnq2 potassium voltage-gated cha... XM_011239925.1 37.6% 41.3% (many diffs)
32 mouse 16536 Kcnq2 potassium voltage-gated cha... XM_017315744.1 37.6% 41.2% (many diffs)
33 mouse 16536 Kcnq2 potassium voltage-gated cha... XM_017315740.1 37.3% 40.2% (many diffs)
34 mouse 16536 Kcnq2 potassium voltage-gated cha... XM_017315742.1 37.1% 40.7% (many diffs)
35 mouse 16536 Kcnq2 potassium voltage-gated cha... XM_017315741.1 36.9% 40.4% (many diffs)
36 mouse 16536 Kcnq2 potassium voltage-gated cha... XM_017315738.1 36.4% 40% (many diffs)
37 mouse 16536 Kcnq2 potassium voltage-gated cha... XM_017315735.1 36.4% 39.9% (many diffs)
38 mouse 16536 Kcnq2 potassium voltage-gated cha... XR_001781086.1 33.5% (many diffs)
39 mouse 16536 Kcnq2 potassium voltage-gated cha... XR_001781085.1 33% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1245
ORF length:
1179
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaat
61ttggcatggt gcagaagtcg cgcaacggcg gcgtataccc cggcccgagc ggggagaaga
121agctgaaggt gggcttcgtg gggctggacc ccggcgcgcc cgactccacc cgggacgggg
181cgctgctgat cgccggctcc gaggccccca agcgcggcag catcctcagc aaacctcgcg
241cgggcggcgc gggcgccggg aagcccccca agcgcaacgc cttctaccgc aagctgcaga
301atttcctcta caacgtgctg gagcggccgc gcggctgggc gttcatctac cacgcctacg
361tgttcctcct ggttttctcc tgcctcgtgc tgtctgtgtt ttccaccatc aaggagtatg
421agaagagctc ggagggggcc ctctacatcc tggaaatcgt gactatcgtg gtgtttggcg
481tggagtactt cgtgcggatc tgggccgcag gctgctgctg ccggtaccgt ggctggaggg
541ggcggctcaa gtttgcccgg aaaccgttct gtgtgattga catcatggtg ctcatcgcct
601ccattgcggt gctggccgcc ggctcccagg gcaacgtctt tgccacatct gcgctccgga
661gcctgcgctt cctgcagatt ctgcggatga tccgcatgga ccggcgggga ggcacctgga
721agctgctggg ctctgtggtc tatgcccaca gcaaggagct ggtcactgcc tggtacatcg
781gcttccttTG TCTCATCCTG GCCTCGTTCC TGGTGTACTT GGCAGAGAAG GGGGAGAACG
841ACCACTTTGA CACCTACGCG GATGCACTCT GGTGGGGCCT GATCACGCTG ACCACCATTG
901GCTACGGGGA CAAGTACCCC CAGACCTGGA ACGGCAGGCT CCTTGCGGCA ACCTTCACCC
961TCATCGGTGT CTCCTTCTTC GCGCTGCCTG CAGGCATCTT GGGGTCTGGG TTTGCCCTGA
1021AGGTTCAGGA GCAGCACAGG CAGAAGCACT TTGAGAAGAG GCGGAACCCG GCAGCAGGCC
1081TGATCCAGTC GGCCTGGAGA TTCTACGCCA CCAACCTCTC GCGCACAGAC CTGCACTCCA
1141CGTGGCAGTA CTACGAGCGA ACGGTCACCG TGCCCATGTA CAGGTACCGC CGCCGGGCAC
1201CTGCCACCAA GCAACTGTTT CATTTTTTAT TTTCCATTTG TTCTTACCCA ACTTTCTTGT
1261ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT
1321AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG
1381GAAAGGACGA GCACTGAAAA AAATGCTCCC ACAGACGCGT TAAGTCgaca atcaacctct
1441ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)