Construct: ORF TRCN0000471892
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006382.1_s317c1
- Derived from:
- ccsbBroadEn_05935
- DNA Barcode:
- AGTTACTATACTAATTTTAATAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CAPZA2 (830)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471892
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 830 | CAPZA2 | capping actin protein of mu... | NM_006136.3 | 99.8% | 100% | 330C>A |
| 2 | mouse | 12343 | Capza2 | capping protein (actin fila... | NM_007604.3 | 93.7% | 98.2% | (many diffs) |
| 3 | mouse | 12340 | Capza1 | capping protein (actin fila... | XM_006500946.2 | 61.5% | 68.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 924
- ORF length:
- 858
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggatctggag gagcagttgt ctgatgaaga gaaggtgcgt atagcagcaa 121 aattcatcat tcatgcccct cctggagaat ttaatgaggt tttcaatgat gttcggttac 181 tgcttaataa tgacaatctt ctcagggaag gagcagccca tgcatttgca cagtataact 241 tggaccagtt tactccagta aaaattgaag gttatgaaga tcaggtattg ataacagaac 301 atggcgactt gggaaatgga aagtttttgg atccaaagaa cagaatctgt tttaaatttg 361 atcacttaag gaaggaggca actgatccaa gaccatgtga agtagaaaat gcagttgaat 421 catggagaac ttcagtagaa actgctctga gagcttacgt aaaagaaCAT TACCCGAATG 481 GAGTCTGCAC TGTGTATGGC AAAAAAATAG ATGGACAGCA AACCATTATT GCATGCATAG 541 AAAGCCATCA GTTCCAAGCA AAAAATTTTT GGAATGGTCG TTGGAGGTCA GAATGGAAGT 601 TTACAATCAC TCCTTCAACC ACTCAAGTGG TTGGCATCTT GAAAATTCAG GTTCATTATT 661 ATGAAGATGG TAATGTTCAG CTAGTGAGTC ATAAAGATAT ACAAGATTCC CTAACAGTGT 721 CTAATGAAGT GCAAACAGCA AAAGAATTTA TAAAGATTGT AGAAGCTGCA GAAAATGAAT 781 ACCAGACTGC CATCAGTGAG AATTATCAGA CAATGTCGGA CACTACTTTC AAAGCCTTAC 841 GTCGACAGTT GCCAGTTACA CGCACTAAGA TTGATTGGAA CAAGATCCTT AGCTACAAGA 901 TTGGCAAAGA GATGCAGAAT GCATACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 961 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1021 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA GTTACTATAC 1081 TAATTTTAAT AAGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1141 att