Construct: ORF TRCN0000471900
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002484.1_s317c1
- Derived from:
- ccsbBroadEn_06770
- DNA Barcode:
- GAGACCCCTGCACCTGCAGGCCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRKAA1 (5562)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471900
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5562 | PRKAA1 | protein kinase AMP-activate... | NM_206907.3 | 99.9% | 99.8% | 28A>T |
| 2 | human | 5562 | PRKAA1 | protein kinase AMP-activate... | NM_006251.5 | 97.3% | 97.2% | 28A>T;362_363ins45 |
| 3 | human | 5562 | PRKAA1 | protein kinase AMP-activate... | XM_017009624.1 | 94.2% | 92% | (many diffs) |
| 4 | human | 5562 | PRKAA1 | protein kinase AMP-activate... | NM_001355028.2 | 91.6% | 89.4% | (many diffs) |
| 5 | human | 5562 | PRKAA1 | protein kinase AMP-activate... | XM_017009625.2 | 91.6% | 89.4% | (many diffs) |
| 6 | human | 5562 | PRKAA1 | protein kinase AMP-activate... | NM_001355029.2 | 72.2% | 72.2% | 0_1ins477 |
| 7 | human | 5562 | PRKAA1 | protein kinase AMP-activate... | NM_001355035.2 | 52.4% | 52.4% | 0_1ins819 |
| 8 | human | 5562 | PRKAA1 | protein kinase AMP-activate... | NM_001355036.2 | 52.4% | 52.4% | 0_1ins819 |
| 9 | human | 5562 | PRKAA1 | protein kinase AMP-activate... | NM_001355037.2 | 52.4% | 52.4% | 0_1ins819 |
| 10 | human | 5562 | PRKAA1 | protein kinase AMP-activate... | NM_001355034.1 | 36.4% | 33.9% | (many diffs) |
| 11 | mouse | 105787 | Prkaa1 | protein kinase, AMP-activat... | NM_001013367.3 | 87.8% | 95.9% | (many diffs) |
| 12 | mouse | 105787 | Prkaa1 | protein kinase, AMP-activat... | XM_011245321.2 | 71.8% | 78.5% | (many diffs) |
| 13 | mouse | 105787 | Prkaa1 | protein kinase, AMP-activat... | XM_017316356.1 | 71.8% | 78.5% | (many diffs) |
| 14 | mouse | 105787 | Prkaa1 | protein kinase, AMP-activat... | XM_006519953.2 | 64.8% | 71% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1788
- ORF length:
- 1722
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcg cagactcagt tcctggagaa agttggcgac agccgagaag cagaaacacg 121 acgggcgggt gaagatcggc cactacattc tgggtgacac gctgggggtc ggcaccttcg 181 gcaaagtgaa ggttggcaaa catgaattga ctgggcataa agtagctgtg aagatactca 241 atcgacagaa gattcggagc cttgatgtgg taggaaaaat ccgcagagaa attcagaacc 301 tcaagctttt caggcatcct catataatta aactgtacca ggtcatcagt acaccatctg 361 atattttcat ggtgatggaa tatgtctcag gaggagagct atttgattat atctgtaaga 421 atggaaggaa atctgatgta cctggagtag taaaaacagg ctccacgaag gagctggatg 481 aaaaagaaag tcggcgtctg ttccaacaga tcctttctgg tgtggattat tgtcacaggc 541 atatggtggt ccatagagat ttgaaacctg aaaatgtcct gcttgatgca cacatgaatg 601 caaagatagc tgattttggt ctttcaaaca tgatgtcaga tggtgaattt ttaagaacaa 661 gttgtggctc acccaactat gctgcaccag aagtaatttc aggaagattg tatgcaggcc 721 cagaggtaga tatatggagc agtggggtta ttctctatgc tttattatgt ggaacccttc 781 catttgatga tgaccatgtg ccaactcttt ttaagaagat atgtgatggg atcttctata 841 cccctcaata tttaaatcct tctgtgatta gccttttgaa acatatgctg caggtggatc 901 ccatgaagag ggccacaatc aaagatatca gggaacatga atggtttaaa caggaccttc 961 caaaatatct ctttcctgag gatccatcat atagttcaac catgattgat gatgaagcct 1021 taaaagaagt atgtgaaaag tttgagtgct cagaagagga agttctcagc tgtctttaca 1081 acagaaatca ccaggatcct ttggcagttg cctaccatct cataatagat aacaggagaa 1141 taatgaatga agccaaagat ttctatttgg cgacaagccc acctgattct tttcttgatg 1201 atcatcacct gactcggccc catcctgaaa gagtaccatt cttggttgct gaaacaccaa 1261 gggcacgcca tacccttgat gaattaaatc cacagaaatC CAAACACCAA GGTGTAAGGA 1321 AAGCAAAATG GCATTTAGGA ATTAGAAGTC AAAGTCGACC AAATGATATT ATGGCAGAAG 1381 TATGTAGAGC AATCAAACAA TTGGATTATG AATGGAAGGT TGTAAACCCA TATTATTTGC 1441 GTGTACGAAG GAAGAATCCT GTGACAAGCA CTTACTCCAA AATGAGTCTA CAGTTATACC 1501 AAGTGGATAG TAGAACTTAT CTACTGGATT TCCGTAGTAT TGATGATGAA ATTACAGAAG 1561 CCAAATCAGG GACTGCTACT CCACAGAGAT CGGGATCAGT TAGCAACTAT CGATCTTGCC 1621 AAAGGAGTGA TTCAGATGCT GAGGCTCAAG GAAAATCCTC AGAAGTTTCT CTTACCTCAT 1681 CTGTGACCTC ACTTGACTCT TCTCCTGTTG ACCTAACTCC AAGACCTGGA AGTCACACAA 1741 TAGAATTTTT TGAGATGTGT GCAAATCTAA TTAAAATTCT TGCACAATAC CCAACTTTCT 1801 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1861 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1921 GTGGAAAGGA CGAGAGACCC CTGCACCTGC AGGCCCTACG CGTTAAGTCg acaatcaacc 1981 tctggattac aaaatttgtg aaagatt