Construct: ORF TRCN0000471909
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009696.1_s317c1
- Derived from:
- ccsbBroadEn_04384
- DNA Barcode:
- GTGATAAGTTCAATAAACATACGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM101 (84336)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471909
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84336 | TMEM101 | transmembrane protein 101 | NM_032376.4 | 100% | 100% | |
| 2 | human | 84336 | TMEM101 | transmembrane protein 101 | NM_001304813.1 | 77.4% | 77.4% | 0_1ins174 |
| 3 | human | 84336 | TMEM101 | transmembrane protein 101 | NM_001304814.1 | 77.4% | 77.4% | 0_1ins174 |
| 4 | human | 84336 | TMEM101 | transmembrane protein 101 | XM_011525353.2 | 77.4% | 77.4% | 0_1ins174 |
| 5 | human | 84336 | TMEM101 | transmembrane protein 101 | XM_024451006.1 | 77.4% | 77.4% | 0_1ins174 |
| 6 | mouse | 76547 | Tmem101 | transmembrane protein 101 | NM_029649.2 | 87.5% | 98% | (many diffs) |
| 7 | mouse | 76547 | Tmem101 | transmembrane protein 101 | XM_017314829.1 | 67% | 75.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 837
- ORF length:
- 771
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gtcgaagata ggttcgagac ggtggatgtt gcagctgatc atgcagttgg 121 gttcggtgct gctcacacgc tgcccctttt ggggctgctt cagccagctc atgctgtacg 181 ctgagagggc tgaggcacgc cggaagcccg acatcccagt gccttacctg tatttcgaca 241 tgggggcagc cgtgctgtgc gctagtttca tgtcctttgg cgtgaagcgg cgctggttcg 301 cgctgggggc cgcactccaa ttggccatta gcacctacgc cgccTACATC GGGGGCTACG 361 TCCACTACGG GGACTGGCTG AAGGTCCGTA TGTACTCGCG CACAGTTGCC ATCATCGGCG 421 GCTTTCTTGT GTTGGCCAGC GGTGCTGGGG AGCTGTACCG CCGGAAACCT CGCAGCCGCT 481 CCCTGCAGTC CACCGGCCAG GTGTTCCTGG GTATCTACCT CATCTGTGTG GCCTACTCAC 541 TGCAGCACAG CAAGGAGGAC CGGCTGGCGT ATCTGAACCA TCTCCCAGGA GGGGAGCTGA 601 TGATCCAGCT GTTCTTCGTG CTGTATGGCA TCCTGGCCCT GGCCTTTCTG TCAGGCTACT 661 ACGTGACCCT CGCTGCCCAG ATCCTGGCTG TACTGCTGCC CCCTGTCATG CTGCTCATTG 721 ATGGCAATGT TGCTTACTGG CACAACACGC GGCGTGTTGA GTTCTGGAAC CAGATGAAGC 781 TCCTTGGAGA GAGTGTGGGC ATCTTCGGAA CTGCTGTCAT CCTGGCCACT GATGGCTGCC 841 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 901 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGTTTT 961 ATATATCTTG TGGAAAGGAC GAGTGATAAG TTCAATAAAC ATACGTACGC GTTAAGTCga 1021 caatcaacct ctggattaca aaatttgtga aagatt