Construct: ORF TRCN0000471914
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011377.1_s317c1
- Derived from:
- ccsbBroadEn_01726
- DNA Barcode:
- ATCAGCGGTGTTGTCGCTGATTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TUFM (7284)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471914
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7284 | TUFM | Tu translation elongation f... | NM_003321.5 | 100% | 100% | |
| 2 | human | 7284 | TUFM | Tu translation elongation f... | NM_001365360.2 | 93.8% | 93.8% | 828_829ins84 |
| 3 | human | 100616256 | MIR4721 | microRNA 4721 | NR_039872.1 | 5.8% | (many diffs) | |
| 4 | mouse | 233870 | Tufm | Tu translation elongation f... | NM_172745.3 | 86.5% | 92.9% | (many diffs) |
| 5 | mouse | 233870 | Tufm | Tu translation elongation f... | XM_006507719.3 | 81% | 87% | (many diffs) |
| 6 | mouse | 233870 | Tufm | Tu translation elongation f... | NM_001163713.1 | 74.1% | 76.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1431
- ORF length:
- 1365
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac cacaatggcg gccgccaccc tgctgcgcgc gacgccccac ttcagcggtc 121 tcgccgccgg ccggaccttc ctgctgcagg gtctgttgcg gctgctgaaa gccccggcat 181 tgcctctctt gtgccgcggc ctggccgtgg aggccaagaa gacttacgtg cgcgacaagc 241 cacatgtgaa tgtgggtacc atcggccatg tggaccacgg gaagaccacg ctgactgcag 301 ccatcacgaa gattctagct gagggaggtg gggctaagtt caagaagtac gaggagattg 361 acaatgcccc ggaggagcga gctcggggta tcaccatcaa tgcggctcat gtggagtata 421 gcactgccgc ccgccactac gcccacacag actgcccggg tcatgcagat tatgttaaga 481 atatgatcac aggcactgca cccctcgacg gctgcatcct ggtggtagca gccaatgacg 541 gccccatgcc ccagacccga gagcacttat tactggccag acagattggg gtggagcatg 601 tggtggtgta tgtgaacaag gctgacgctg tccaggactc tgagatggtg gaactggtgg 661 aactggagat ccgggagctg ctcaccgagt ttggctataa aggggaggag accccagtca 721 tcgtaggctc tgctctctgt gcccttgagg gtcgggaccc tgagttaggc ctgaagtctg 781 tgcagaagct actggatgct gtggacactt acatcccagt gcccgcccgg gacctggaga 841 agcctttcct gctgcctgtg gaggcggtgt actccgtccc tggccgtggc accgtggtga 901 caggtacact agagcgtggc attttaaaga agggagacga gtgtgagctc cTAGGACATA 961 GCAAGAACAT CCGCACTGTG GTGACAGGCA TTGAGATGTT CCACAAGAGC CTGGAGAGGG 1021 CCGAGGCCGG AGATAACCTC GGGGCCCTGG TCCGAGGCTT GAAGCGGGAG GACTTGCGGC 1081 GGGGCCTGGT CATGGTCAAG CCAGGTTCCA TCAAGCCCCA CCAGAAGGTG GAGGCCCAGG 1141 TTTACATCCT CAGCAAGGAG GAAGGTGGCC GCCACAAGCC CTTTGTGTCC CACTTCATGC 1201 CTGTCATGTT CTCCCTGACT TGGGACATGG CCTGTCGGAT TATCCTGCCC CCAGAGAAGG 1261 AGCTTGCCAT GCCCGGGGAG GACCTGAAGT TCAACCTAAT CTTGCGGCAG CCAATGATCT 1321 TAGAGAAAGG CCAGCGTTTC ACCCTGCGAG ATGGCAACCG GACTATTGGC ACCGGTCTAG 1381 TCACCAACAC GCTGGCCATG ACTGAGGAGG AGAAGAATAT CAAATGGGGT TGCCCAACTT 1441 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1501 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1561 CTTGTGGAAA GGACGAATCA GCGGTGTTGT CGCTGATTTA ACGCGTTAAG TCgacaatca 1621 acctctggat tacaaaattt gtgaaagatt