Construct: ORF TRCN0000471918
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019039.3_s317c1
- Derived from:
- ccsbBroadEn_14684
- DNA Barcode:
- CTATACACTAATTATCGGTAATCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KDR (3791)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471918
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3791 | KDR | kinase insert domain receptor | NM_002253.3 | 100% | 100% | |
| 2 | mouse | 16542 | Kdr | kinase insert domain protei... | NM_010612.2 | 84.7% | 85.7% | (many diffs) |
| 3 | mouse | 16542 | Kdr | kinase insert domain protei... | XM_011240817.2 | 73.2% | 74.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 4137
- ORF length:
- 4068
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcagagcaag gtgctgctgg ccgtcgccct gtggctctgc gtggagaccc 121 gggccgcctc tgtgggtttg cctagtgttt ctcttgatct gcccaggctc agcatacaaa 181 aagacatact tacaattaag gctaatacaa ctcttcaaat tacttgcagg ggacagaggg 241 acttggactg gctttggccc aataatcaga gtggcagtga gcaaagggtg gaggtgactg 301 agtgcagcga tggcctcttc tgtaagacac tcacaattcc aaaagtgatc ggaaatgaca 361 ctggagccta caagtgcttc taccgggaaa ctgacttggc ctcggtcatt tatgtctatg 421 ttcaagatta cagatctcca tttattgctt ctgttagtga ccaacatgga gtcgtgtaca 481 ttactgagaa caaaaacaaa actgtggtga ttccatgtct cgggtccatt tcaaatctca 541 acgtgtcact ttgtgcaaga tacccagaaa agagatttgt tcctgatggt aacagaattt 601 cctgggacag caagaagggc tttactattc ccagctacat gatcagctat gctggcatgg 661 tcttctgtga agcaaaaatt aatgatgaaa gttaccagtc tattatgtac atagttgtcg 721 ttgtagggta taggatttat gatgtggttc tgagtccgtc tcatggaatt gaactatctg 781 ttggagaaaa gcttgtctta aattgtacag caagaactga actaaatgtg gggattgact 841 tcaactggga atacccttct tcgaagcatc agcataagaa acttgtaaac cgagacctaa 901 aaacccagtc tgggagtgag atgaagaaat ttttgagcac cttaactata gatggtgtaa 961 cccggagtga ccaaggattg tacacctgtg cagcatccag tgggctgatg accaagaaga 1021 acagcacatt tgtcagggtc catgaaaaac cttttgttgc ttttggaagt ggcatggaat 1081 ctctggtgga agccacggtg ggggagcgtg tcagaatccc tgcgaagtac cttggttacc 1141 cacccccaga aataaaatgg tataaaaatg gaatacccct tgagtccaat cacacaatta 1201 aagcggggca tgtactgacg attatggaag tgagtgaaag agacacagga aattacactg 1261 tcatccttac caatcccatt tcaaaggaga agcagagcca tgtggtctct ctggttgtgt 1321 atgtcccacc ccagattggt gagaaatctc taatctctcc tgtggattcc taccagtacg 1381 gcaccactca aacgctgaca tgtacggtct atgccattcc tcccccgcat cacatccact 1441 ggtattggca gttggaggaa gagtgcgcca acgagcccag ccaagctgtc tcagtgacaa 1501 acccataccc ttgtgaagaa tggagaagtg tggaggactt ccagggagga aataaaattg 1561 aagttaataa aaatcaattt gctctaattg aaggaaaaaa caaaactgta agtacccttg 1621 ttatccaagc ggcaaatgtg tcagctttgt acaaatgtga agcggtcaac aaagtcggga 1681 gaggagagag ggtgatctcc ttccacgtga ccaggggtcc tgaaattact ttgcaacctg 1741 acatgcagcc cactgagcag gagagcgtgt ctttgtggtg cactgcagac agatctacgt 1801 ttgagaacct cacatggtac aagcttggcc cacagcctct gccaatccat gtgggagagt 1861 tgcccacacc tgtttgcaag aacttggata ctctttggaa attgaatgcc accatgttct 1921 ctaatagcac aaatgacatt ttgatcatgg agcttaagaa tgcatccttg caggaccaag 1981 gagactatgt ctgccttgct caagacagga agaccaagaa aagacattgc gtggtcaggc 2041 agctcacagt cctagagcgt gtggcaccca cgatcacagg aaacctggag aatcagacga 2101 caagtattgg ggaaagcatc gaagtctcat gcacggcatc tgggaatccc cctccacaga 2161 tcatgtggtt taaagataat gagacccttg tagaagactc aggcattgta ttgaaggatg 2221 ggaaccggaa cctcactatc cgcagagtga ggaaggagga cgaaggcctc tacacctgcc 2281 aggcatgcag tgttcttggc tgtgcaaaag tggaggcatt tttcataata gaaggtgccc 2341 aggaaaagac gaacttggaa atcattattc tagtaggcac ggcggtgatt gccatgttct 2401 tctggctact tcttgtcatc atcctacgga ccgttaagcg ggccaatgga ggggaactga 2461 agacaggcta cttgtccatc gtcatggatc cagatgaact cccattggat gaacattgtg 2521 aacgactgcc ttatgatgcc agcaaatggg aattccccag agaccggctg aagctaggta 2581 agcctcttgg ccgtggtgcc tttggccaag tgattgaagc agatgccttt ggaattgaca 2641 agacagcaac ttgcaggaca gtagcagtca aaatgttgaa agaaggagca acacacagtg 2701 agcatcgagc tctcatgtct gaactcaaga tcctcattca tattggtcac catctcaatg 2761 tggtcaacct tctaggtgcc tgtaccaagc caggagggcc actcatggtg attgtggaat 2821 tctgcaaatt tggaaacctg tccacttacc tgaggagcaa gagaaatgaa tttgtcccct 2881 acaagaccaa aggggcacga ttccgtcaag ggaaagacta cgttggagca atccctgtgg 2941 atctgaaacg gcgcttggac agcatcacca gtagccagag ctcagccagc tctggatttg 3001 tggaggagaa gtccctcagt gatgtagaag aagaggaagc tcctgaagat ctgtataagg 3061 acttcctgac cttggagcat ctcatctgtt acagcttcca agtggctaag ggcatggagt 3121 tcttggcatc gcgaaagtgt atccacaggg acctggcggc acgaaatatc ctcttatcgg 3181 agaagaacgt ggttaaaatc tgtgactttg gcttggcccg ggatatttat aaagatccag 3241 attatgtcag aaaaggagat gctcgcctcc ctttgaaatg gatggcccca gaaacaattt 3301 ttgacagagt gtacacaatc cagagtgacg tctggtcttt tggtgttttg ctgtgggaaa 3361 tattttcctt aggtgcttct ccatatcctg gggtaaagat tgatgaagaa ttttgtaggc 3421 gattgaaaga aggaactaga atgagggccc ctgattatac tacaccagaa atgtaccaga 3481 ccatgctgga ctgctggcac ggggagccca gtcagagacc cacgttttca gagttggtgg 3541 aacatttggg aaatctcttg caagctaatg ctcagcagga tggcaaagac tacattgttc 3601 ttccgatatc agagactttg agcatggaag aggattctgg actctctctg cctaccTCAC 3661 CTGTTTCCTG TATGGAGGAG GAGGAAGTAT GTGACCCCAA ATTCCATTAT GACAACACAG 3721 CAGGAATCAG TCAGTATCTG CAGAACAGTA AGCGAAAGAG CCGGCCTGTG AGTGTAAAAA 3781 CATTTGAAGA TATCCCGTTA GAAGAACCAG AAGTAAAAGT AATCCCAGAT GACAACCAGA 3841 CGGACAGTGG TATGGTTCTT GCCTCAGAAG AGCTGAAAAC TTTGGAAGAC AGAACCAAAT 3901 TATCTCCATC TTTTGGTGGA ATGGTGCCCA GCAAAAGCAG GGAGTCTGTG GCATCTGAAG 3961 GCTCAAACCA GACAAGCGGC TACCAGTCCG GATATCACTC CGATGACACA GACACCACCG 4021 TGTACTCCAG TGAGGAAGCA GAACTTTTAA AGCTGATAGA GATTGGAGTG CAAACCGGTA 4081 GCACAGCCCA GATTCTCCAG CCTGACTCGG GGACCACACT GAGCTCTCCT CCTGTTTTGC 4141 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 4201 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 4261 ATATATCTTG TGGAAAGGAC GACTATACAC TAATTATCGG TAATCGACGC GTTAAGTCga 4321 caatcaacct ctggattaca aaatttgtga aagatt