Construct: ORF TRCN0000471929
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013336.1_s317c1
- Derived from:
- ccsbBroadEn_00111
- DNA Barcode:
- GGATATTAACCTTCGAGTTGGTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ASIP (434)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471929
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 434 | ASIP | agouti signaling protein | NM_001672.2 | 100% | 100% | |
| 2 | human | 434 | ASIP | agouti signaling protein | XM_011528820.2 | 100% | 100% | |
| 3 | human | 434 | ASIP | agouti signaling protein | XM_011528821.1 | 100% | 100% | |
| 4 | human | 434 | ASIP | agouti signaling protein | XM_005260412.3 | 97% | 97% | 1_12del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 465
- ORF length:
- 396
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggatgtcacc cgcttactcc tggccaccct gctggtcttc ctctgcttct 121 tcactgccaa cagccacctg ccaccTGAGG AGAAGCTCCG AGATGACAGG AGCCTGAGAA 181 GCAACTCCTC TGTGAACCTA CTGGATGTCC CTTCTGTCTC TATTGTGGCG CTGAACAAGA 241 AATCCAAACA GATCGGCAGA AAAGCAGCAG AAAAGAAAAG ATCTTCTAAG AAGGAGGCTT 301 CGATGAAGAA AGTGGTGCGG CCCCGGACCC CCCTATCTGC GCCCTGCGTG GCCACCCGCA 361 ACAGCTGCAA GCCGCCGGCA CCCGCCTGCT GCGACCCGTG CGCCTCCTGC CAGTGCCGCT 421 TCTTCCGCAG CGCCTGCTCC TGCCGCGTGC TCAGCCTCAA CTGCTTGCCA ACTTTCTTGT 481 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 541 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 601 GAAAGGACGA GGATATTAAC CTTCGAGTTG GTTTACGCGT TAAGTCgaca atcaacctct 661 ggattacaaa atttgtgaaa gatt