Construct: ORF TRCN0000471937
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015757.1_s317c1
- Derived from:
- ccsbBroadEn_08983
- DNA Barcode:
- ACCCCCAACCTTGAGAAGCTACAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMC7 (79905)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471937
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79905 | TMC7 | transmembrane channel like 7 | NM_024847.4 | 99.9% | 99.8% | 175C>T;1941G>A |
| 2 | human | 79905 | TMC7 | transmembrane channel like 7 | NM_001300732.1 | 93.2% | 92.8% | (many diffs) |
| 3 | human | 79905 | TMC7 | transmembrane channel like 7 | NM_001324265.1 | 92.7% | 92.6% | 175C>T;1870_1871ins156 |
| 4 | human | 79905 | TMC7 | transmembrane channel like 7 | NM_001160364.2 | 84.7% | 84.7% | 0_1ins330;1611G>A |
| 5 | human | 79905 | TMC7 | transmembrane channel like 7 | NM_001324263.1 | 52.6% | 52.6% | 0_1ins1026;915G>A |
| 6 | human | 79905 | TMC7 | transmembrane channel like 7 | NM_001324268.1 | 52.6% | 52.6% | 0_1ins1026;915G>A |
| 7 | human | 79905 | TMC7 | transmembrane channel like 7 | NR_136733.1 | 45.4% | (many diffs) | |
| 8 | mouse | 209760 | Tmc7 | transmembrane channel-like ... | NM_172476.4 | 84.8% | 90.3% | (many diffs) |
| 9 | mouse | 209760 | Tmc7 | transmembrane channel-like ... | XM_006507550.3 | 72.9% | 78.7% | (many diffs) |
| 10 | mouse | 209760 | Tmc7 | transmembrane channel-like ... | XR_001785514.1 | 40.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2235
- ORF length:
- 2169
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag cgagtccagc ggcagtgcgc tccagcccgg caggcccagc cggcagccgg 121 cggtccatcc agagaacctc tctctagact ccagttgctt ctcttctcca cctgtgaact 181 tcctccaaga attgccaagc taccggtcca ttgcacgtag gagaacgact gtccattcct 241 gggacaagca aagcggaact ttgctaaagc caaccgactc ttacagctcc cagctggagg 301 acagaatcgc tgaaaacctc agcagccatt ctcttcgaaa ttatgcactg aacatctctg 361 agaagcggag actaagggac attcaagaga cacaaatgaa gtatctctcc gaatgggacc 421 agtggaagcg gtatagcagc aagtcttgga agaggttcct agagaaggct cgagagatga 481 cgacccacct ggagctgtgg cgggaggaca tccgcagcat agaagggaaa tttggcactg 541 ggattcagtc ctatttctcc ttcttgagat tcctggtgtt gctgaatttg gtgatatttc 601 tgatcatctt tatgctggtt ttgctcccag tcttactcac gaaatacaag atcaccaaca 661 gcagcttcgt gctcattcct ttcaaagaca tggataaaca atgtacagtc tatccagtaa 721 gcagttctgg actcatttac ttttacagtt atatcataga cttgctttct ggcactggtt 781 tcctggagga aactagcctc ttttacggac attacaccat tgatggggtg aaatttcaga 841 acttcaccta tgatctgccc ctggcgtatt tgttaagcac aatcgcctcc ctggccctga 901 gccttctttg gatagtgaaa aggtcggtgg aaggattcaa aatcaacctg attcggagtg 961 aggagcactt tcagagttac tgcaacaaga tatttgccgg ctgggacttc tgcatcacta 1021 accgcagcat ggcggatctg aagcacagca gcttgcggta cgagctccga gcagatctgg 1081 aggaagaaag aatgcggcag aaaatagcag aaaggacctc agaagaaaca atacgcattt 1141 actctttgag actgtttttg aactgtattg ttctggctgt tttaggggca tgcttttatg 1201 caatatacgt agcaactgtc ttctcgcaag agcacatgaa aaaggaaatc gacaagatgg 1261 tttttggaga gaacctcttc atattgtatc taccgtctat tgtgatcacg ctggccaatt 1321 ttatcacccc aatgatcttt gccaagatca tccgctatga ggattattct ccaggctttg 1381 agatccgtct gacaatcctt aggtgtgtct ttatgcggct ggccaccata tgtgtcctgg 1441 tgttcacgct gggctccaag atcacatcct gtgatgatga cacatgtgac ctttgcggct 1501 acaaccagaa actctacccg tgctgggaga cccaagttgg gcaggaaatg tacaagctga 1561 tgatcttcga cttcatcatc atcttggctg tgacactctt cgtggatttt cctagaaagc 1621 tcctggtgac ctactgttcc tcttgcaagc tgattcagtg ctgggggcag caggagtttg 1681 ccattcctga taacgtcctg gggatagttt acgggcaaac catctgctgg atcggagcct 1741 ttttctcacc ccttctccct gcaattgcaa ccctgaaatt cattatcatc ttctatgtga 1801 aagagtggag tctgctttac acctgcagac cctcccccag gccgttcaga gcatccaatt 1861 ctaattTCTT CTTCCTGTTG GTGTTGTTGA TCGGGCTGTG TTTGGCAATA ATACCTCTGA 1921 CAATCAGCAT ATCACGCATC CCTTCCTCGA AAGCCTGTGG GCCGTTCACC AACTTCAACA 1981 CCACCTGGGA GGTCATCCCC AAGACAGTGA GCACCTTCCC CAGCTCGCTG CAGTCCTTCA 2041 TCCATGGTGT CACATCCGAA GCCTTTGCAG TTCCTTTCTT CATGATTATT TGCCTCATCA 2101 TGTTTTACTT CATTGCCTTA GCTGGAGCAC ACAAACGGGT GGTCATCCAG CTCCGAGAGC 2161 AGCTATCCCT GGAAAGTCGT GACAAGTGCT ACCTAATCCA GAAACTAACA GAAGCCCAAA 2221 GGGACATGAG GAACTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 2281 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 2341 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA ACCCCCAACC TTGAGAAGCT 2401 ACACACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt