Construct: ORF TRCN0000471945

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011963.1_s317c1
Derived from:
ccsbBroadEn_04154
DNA Barcode:
CTACGATTTATAGTCTTTGTGACC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DENND2D (79961)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000471945
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 79961 DENND2D DENN domain containing 2D NM_024901.5 100% 100%
2 human 79961 DENND2D DENN domain containing 2D NM_001271833.1 97.2% 96.8% (many diffs)
3 human 79961 DENND2D DENN domain containing 2D XM_011542187.1 95.5% 95.3% (many diffs)
4 human 79961 DENND2D DENN domain containing 2D XM_011542190.2 95.5% 95.3% (many diffs)
5 human 79961 DENND2D DENN domain containing 2D XM_006710921.2 94.4% 93.8% (many diffs)
6 human 79961 DENND2D DENN domain containing 2D XM_006710922.1 79.7% 67.9% (many diffs)
7 human 79961 DENND2D DENN domain containing 2D XM_006710923.1 79.7% 67.9% (many diffs)
8 human 79961 DENND2D DENN domain containing 2D XM_006710924.1 79.7% 67.9% (many diffs)
9 human 79961 DENND2D DENN domain containing 2D XM_011542189.1 79.7% 67.9% (many diffs)
10 human 79961 DENND2D DENN domain containing 2D XM_024449890.1 79.7% 67.9% (many diffs)
11 human 79961 DENND2D DENN domain containing 2D XM_024449891.1 79.7% 67.9% (many diffs)
12 human 79961 DENND2D DENN domain containing 2D XR_946764.1 66.1% (many diffs)
13 human 79961 DENND2D DENN domain containing 2D XM_017002386.1 61.7% 61.7% 0_1ins540
14 human 79961 DENND2D DENN domain containing 2D XM_017002387.1 61.7% 61.7% 0_1ins540
15 human 79961 DENND2D DENN domain containing 2D XM_017002388.1 61.7% 61.7% 0_1ins540
16 human 79961 DENND2D DENN domain containing 2D XM_024449898.1 61.7% 61.7% 0_1ins540
17 human 79961 DENND2D DENN domain containing 2D XM_017002389.1 42.6% 41.4% (many diffs)
18 human 79961 DENND2D DENN domain containing 2D XR_946765.1 40.3% (many diffs)
19 mouse 72121 Dennd2d DENN/MADD domain containing 2D NM_001093754.2 85.4% 89.3% (many diffs)
20 mouse 72121 Dennd2d DENN/MADD domain containing 2D NM_028110.2 85.3% 89.5% (many diffs)
21 mouse 72121 Dennd2d DENN/MADD domain containing 2D XM_006502133.3 73.3% 69.7% (many diffs)
22 mouse 72121 Dennd2d DENN/MADD domain containing 2D XR_867235.2 58.6% (many diffs)
23 mouse 72121 Dennd2d DENN/MADD domain containing 2D NM_001278941.1 54.2% 57.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1479
ORF length:
1413
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga aggacaagtg gtaggccggg tgttcaggct cttccaacgc cgactgcttc
121aactccgagc aggaccaccc caggacaatt caggggaagc tttaaaggaa ccagaaaggg
181cccaggagca ctctttgccc aactttgctg gggggcagca cttctttgaa taccttcttg
241tggtttctct caaaaagaag cgttcagagg atgattacga gcctataatc acctaccaat
301ttcccaagcg ggagaacctg cttcggggtc agcaggagga ggaggagcgg ctgctcaaag
361ctatcccctt gttctgcttc ccagatggga atgagtgggc atcactcacc gagtatccca
421gggagacctt ctccttcgtt ctgaccaatg tggatgggag cagaaagatt ggatactgca
481ggcgcctctt gcctgccggc cctggccctc gccttcccaa agtgtactgc atcatcagct
541gcatcggctg cttcggcttg ttctccaaga tcctggatga agtggagaag agacatcaga
601tctccatggc tgtcatctac ccgttcatgc agggcctccg agaggcagcc ttccctgctc
661ctgggaagac tgtcactctc aagagcttca tccccgactc aggcactgag ttcatttcac
721tgacacggcc cctggactcc cacctagaac atgtggattt tagttctcta ttgcactgtc
781tcagttttga acagatactt cagatctttg cctctgccgt gctggagaga aaaatcatct
841tcctggcgga aggtctcagc accttgtctc agtgcatcca tgctgctgcc gcactgctct
901accccttcag ctgggcgcac acctacatcc ctgttgtccc tgagagcctt ctggccaccg
961tctgctgccc cacccccttc atggttggag tacaaatgcg cttccagcag gaggtcatgg
1021acagccctat ggaagaggtc ctgctggtca atctttgtga aggaaccttc TTAATGTCGG
1081TTGGTGATGA AAAAGACATC CTGCCACCGA AGCTTCAGGA TGACATCTTA GACTCTCTTG
1141GTCAGGGGAT CAATGAGTTA AAGACTGCAG AACAAATCAA CGAGCATGTT TCAGGCCCCT
1201TTGTGCAGTT CTTTGTCAAG ATTGTGGGCC ATTATGCTTC CTATATCAAG CGGGAGGCAA
1261ATGGGCAAGG CCACTTCCAA GAAAGATCCT TCTGTAAGGC TCTGACCTCC AAGACCAACC
1321GCCGATTTGT GAAGAAGTTT GTGAAGACAC AGCTCTTCTC ACTTTTCATC CAGGAAGCCG
1381AGAAGAGCAA GAATCCTCCT GCAGGCTATT TCCAACAGAA AATACTTGAA TATGAGGAAC
1441AGAAGAAACA GAAGAAACCA AGGGAAAAAA CTGTGAAATA CCCAACTTTC TTGTACAAAG
1501TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT
1561GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG
1621ACGACTACGA TTTATAGTCT TTGTGACCAC GCGTTAAGTC gacaatcaac ctctggatta
1681caaaatttgt gaaagatt

Download FASTA (ORF) (Full)