Construct: ORF TRCN0000471953
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004841.1_s317c1
- Derived from:
- ccsbBroadEn_08336
- DNA Barcode:
- CAGTGCCAATTCCCTCCAACCACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HSPB11 (51668)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471953
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51668 | HSPB11 | heat shock protein family B... | NM_001316935.1 | 99.7% | 99.3% | 5G>T |
| 2 | human | 51668 | HSPB11 | heat shock protein family B... | NM_016126.3 | 99.7% | 99.3% | 5G>T |
| 3 | human | 51668 | HSPB11 | heat shock protein family B... | NR_133633.1 | 47.5% | (many diffs) | |
| 4 | human | 51668 | HSPB11 | heat shock protein family B... | NR_133632.1 | 31.5% | (many diffs) | |
| 5 | mouse | 72938 | Hspb11 | heat shock protein family B... | NM_028394.2 | 86.8% | 84% | (many diffs) |
| 6 | mouse | 72938 | Hspb11 | heat shock protein family B... | XM_006503424.3 | 86.8% | 84% | (many diffs) |
| 7 | mouse | 72938 | Hspb11 | heat shock protein family B... | XM_006503425.1 | 86.8% | 84% | (many diffs) |
| 8 | mouse | 72938 | Hspb11 | heat shock protein family B... | XM_006503426.2 | 86.8% | 84% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 498
- ORF length:
- 432
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat aaaaattgat ctctgtctga gcTCTGAAGG GTCCGAAGTG ATTTTAGCTA 121 CATCAAGTGA TGAAAAACAC CCACCTGAAA ATATCATTGA TGGGAATCCA GAAACGTTTT 181 GGACCACCAC AGGAATGTTT CCCCAGGAAT TCATTATTTG TTTCCACAAA CATGTAAGGA 241 TTGAAAGGCT TGTAATCCAA AGTTACTTTG TACAGACCTT GAAGATTGAA AAAAGCACGT 301 CTAAAGAGCC AGTTGATTTT GAGCAATGGA TTGAAAAAGA TTTGGTACAC ACAGAGGGGC 361 AGCTTCAAAA TGAAGAAATT GTGGCACATG ATGGCTCCGC TACTTACTTG AGATTCATTA 421 TTGTATCAGC CTTTGATCAT TTTGCATCTG TGCATAGCGT TTCTGCAGAA GGAACAGTAG 481 TCTCAAATCT TTCCTCATAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 541 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 601 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACAGTGCC AATTCCCTCC 661 AACCACCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt