Construct: ORF TRCN0000471958
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017691.1_s317c1
- Derived from:
- ccsbBroadEn_14013
- DNA Barcode:
- TAAAAAGCCTGGGATATAAAGATA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- CLDN2 (9075)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471958
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9075 | CLDN2 | claudin 2 | NM_001171092.1 | 99.8% | 3.4% | 13delG |
| 2 | human | 9075 | CLDN2 | claudin 2 | NM_001171095.1 | 99.8% | 3.4% | 13delG |
| 3 | human | 9075 | CLDN2 | claudin 2 | NM_020384.4 | 99.8% | 3.4% | 13delG |
| 4 | mouse | 12738 | Cldn2 | claudin 2 | NM_016675.4 | 86.9% | 2.5% | (many diffs) |
| 5 | mouse | 12738 | Cldn2 | claudin 2 | XM_006528490.3 | 86.9% | 2.5% | (many diffs) |
| 6 | mouse | 12738 | Cldn2 | claudin 2 | XM_006528486.3 | 77.7% | 2.3% | (many diffs) |
| 7 | mouse | 12738 | Cldn2 | claudin 2 | XM_006528487.3 | 77.7% | 2.3% | (many diffs) |
| 8 | mouse | 12738 | Cldn2 | claudin 2 | XM_006528488.2 | 77.7% | 2.3% | (many diffs) |
| 9 | mouse | 12738 | Cldn2 | claudin 2 | XM_006528489.3 | 77.7% | 2.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 102
- ORF length:
- 36
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ctctcttgcc tccaacttgt gggctacatc ctaggccttc tggggctttt 121 gggcacactg gttgccatgc tgctccccag ctggaaaaca agttcttatg tcggtgccag 181 cattgtgaca gcagttggct tctccaaggg cctctggatg gaatgtgcca cacacagcac 241 aggcatcacc cagtgtgaca tctatagcac ccttctgggc ctgcccgctg acatccaggc 301 tgcccaggcc atgatggtga catccagtgc aatcTCCTCC CTGGCCTGCA TTATCTCTGT 361 GGTGGGCATG AGATGCACAG TCTTCTGCCA GGAATCCCGA GCCAAAGACA GAGTGGCGGT 421 AGCAGGTGGA GTCTTTTTCA TCCTTGGAGG CCTCCTGGGA TTCATTCCTG TTGCCTGGAA 481 TCTTCATGGG ATCCTACGGG ACTTCTACTC ACCACTGGTG CCTGACAGCA TGAAATTTGA 541 GATTGGAGAG GCTCTTTACT TGGGCATTAT TTCTTCCCTG TTCTCCCTGA TAGCTGGAAT 601 CATCCTCTGC TTTTCCTGCT CATCCCAGAG AAATCGCTCC AACTACTACG ATGCCTACCA 661 AGCCCAACCT CTTGCCACAA GGAGCTCTCC AAGGCCTGGT CAACCTCCCA AAGTCAAGAG 721 TGAGTTCAAT TCCTACAGCC TGACAGGGTA TGTGTGCCCA ACTTTCTTGT ACAAAGTGGT 781 TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC 841 TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA 901 TAAAAAGCCT GGGATATAAA GATAACGCGT TAAGTCgaca atcaacctct ggattacaaa 961 atttgtgaaa gatt