Construct: ORF TRCN0000471963
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004543.1_s317c1
- Derived from:
- ccsbBroadEn_00506
- DNA Barcode:
- ACGCAATGGTGGATGTGTCTTCTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EPB42 (2038)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471963
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2038 | EPB42 | erythrocyte membrane protei... | NM_001114134.1 | 100% | 100% | |
| 2 | human | 2038 | EPB42 | erythrocyte membrane protei... | NM_000119.3 | 95.5% | 95.5% | 1_93del;97_98insGAC;99T>G |
| 3 | human | 2038 | EPB42 | erythrocyte membrane protei... | XM_011521349.2 | 95.5% | 95.5% | 1_93del;97_98insGAC;99T>G |
| 4 | human | 2038 | EPB42 | erythrocyte membrane protei... | XM_011521350.2 | 95.5% | 95.5% | 1_93del;97_98insGAC;99T>G |
| 5 | human | 2038 | EPB42 | erythrocyte membrane protei... | XM_011521351.2 | 95.5% | 95.5% | 1_93del;97_98insGAC;99T>G |
| 6 | human | 2038 | EPB42 | erythrocyte membrane protei... | XM_005254225.1 | 94.9% | 94.9% | 548_549ins105 |
| 7 | human | 2038 | EPB42 | erythrocyte membrane protei... | XM_011521352.2 | 93.8% | 93.8% | (many diffs) |
| 8 | human | 2038 | EPB42 | erythrocyte membrane protei... | XM_011521353.2 | 90.6% | 90.7% | (many diffs) |
| 9 | human | 2038 | EPB42 | erythrocyte membrane protei... | XM_011521354.2 | 77.5% | 77.5% | 0_1ins465 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2142
- ORF length:
- 2073
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggacaggcc ctgggtatca agagctgtga ctttcaggca gcaagaaaca 121 atgaggagca ccacaccaag gccctcagct cccggcgcct ctttgtgagg agggggcagc 181 ccttcaccat catcctgtac ttccgcgctc cagtccgtgc atttctgcct gccctgaaga 241 aggtggccct cactgcacaa actggagagc agccttccaa gatcaacagg acccaagcca 301 cattcccaat ttccagtctg ggggaccgaa agtggtggag tgcagtggtg gaggagagag 361 atgcccagtc ctggaccatc tctgtgacca cacctgcgga cgctgtcatt ggccactact 421 cgcttctgct gcaggtctca ggcaggaagc aactcctctt gggtcagttc acactgcttt 481 ttaacccctg gaatagagag gatgctgtgt tcctgaagaa tgaggctcag cgcatggagt 541 acttgttgaa ccagaatggt ctcatctacc tgggtacagc tgactgcatc caggcagagt 601 cctgggactt tggccagttc gagggggatg tcattgacct cagcctgcgc ttgctgagca 661 aggacaagca ggtagagaag tggagccagc cggtgcacgt ggcccgtgtg ttgggtgcct 721 tgctgcattt tctcaaggag cagagggtcc tgcccacccc gcagacccag gccacccagg 781 aaggggcctt gctgaacaag cgccggggca gcgtgcccat cctgcggcag tggctcaccg 841 gccgaggccg acctgtgtat gatggccagg cctgggtgtt ggctgctgtt gcttgcacag 901 tgctgcgatg cctgggaatc cctgcccgcg tggtgaccac gtttgcctca gcacagggca 961 ccggtgggcg tcttctcata gatgaatact ataatgagga gggacttcag aacggagaag 1021 gccagagagg cagaatctgg atcttccaga cttccacaga gtgctggatg acgcggcctg 1081 ccttgcccca gggttatgat ggatggcaga ttctgcaccc aagtgctcct aatggaggtg 1141 gagtcctggg gtcctgtgat ctggtgccgg tcagagcagt caaggagggg acgctggggc 1201 tgaccccagc agtgtcagac ctttttgctg ccataaatgc ctcatgtgtg gtctggaagt 1261 gctgtgagga tgggacactg gagttgactg actccaacac aaagtatgtt ggcaacaaca 1321 tcagcaccaa gggtgtgggc agtgaccgct gcgaggacat cactcagaac tacaagtatc 1381 ctgaagggtc tcttcaggaa aaagaggtgc tggagagagt cgagaaagag aaaatggaac 1441 gtgagaaaga caacggcatc cgtcctccca gtctcgagac tgccagtcct ctgtacctgc 1501 tcttgaaagc acccagctcc ctacccctga gaggggatgc ccagatctca gtgacgctgg 1561 ttaatcacag tgagcaggag aaggcagtgc agctggcaat tggggtccag gctgtacact 1621 acaacggtgt ccttgctgcc aagctctgga ggaagaagct gcacctcacg ctcagtgcca 1681 acctggaaaa gataataacc atcggcctgt tcttctccaa ttttgagcga aacccacccg 1741 agaacacctt ccttagactc accgccatgg caacacactc tgaatccaac cTTAGCTGCT 1801 TTGCTCAGGA AGACATTGCC ATTTGTAGAC CACACCTTGC CATCAAGATG CCAGAGAAAG 1861 CAGAGCAGTA TCAACCCCTC ACAGCCTCAG TCAGCCTCCA GAACTCCCTA GATGCCCCCA 1921 TGGAGGACTG TGTGATCTCC ATCCTGGGAA GGGGGCTCAT TCACAGAGAG AGGAGCTACA 1981 GATTCCGTTC AGTGTGGCCT GAAAACACCA TGTGTGCCAA GTTCCAGTTC ACGCCAACAC 2041 ATGTGGGGCT CCAGAGACTC ACTGTGGAAG TGGACTGCAA CATGTTCCAG AACCTAACCA 2101 ACTATAAAAG CGTCACCGTG GTAGCCCCTG AACTATCAGC TTTGCCAACT TTCTTGTACA 2161 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 2221 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 2281 AGGACGAACG CAATGGTGGA TGTGTCTTCT GACGCGTTAA GTCgacaatc aacctctgga 2341 ttacaaaatt tgtgaaagat t