Construct: ORF TRCN0000471977
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000311.1_s317c1
- Derived from:
- ccsbBroadEn_02434
- DNA Barcode:
- ATGACTGTGTTAGGCGGCTCACGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TIMM17A (10440)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471977
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10440 | TIMM17A | translocase of inner mitoch... | NM_006335.3 | 100% | 100% | |
| 2 | mouse | 21854 | Timm17a | translocase of inner mitoch... | NM_011590.2 | 89% | 95.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 579
- ORF length:
- 513
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggagtacgcg cgagagcctt gcccatggcg aattgtggat gactgtggtg 121 gggcctttac gatggGTACC ATTGGTGGTG GTATCTTTCA AGCAATCAAA GGTTTTCGCA 181 ATTCTCCAGT GGGAGTAAAC CACAGACTAC GAGGGAGTTT GACAGCTATT AAAACCAGGG 241 CTCCACAGTT AGGAGGTAGC TTTGCAGTTT GGGGAGGGCT GTTTTCCATG ATTGACTGTA 301 GTATGGTTCA AGTCAGAGGA AAGGAAGATC CCTGGAACTC CATCACAAGT GGTGCCTTAA 361 CGGGAGCCAT ACTGGCAGCA AGAAATGGAC CAGTGGCCAT GGTTGGGTCA GCCGCAATGG 421 GTGGCATTCT CCTAGCTTTA ATTGAAGGAG CTGGTATCTT GTTGACAAGA TTTGCCTCTG 481 CACAGTTTCC CAATGGTCCT CAGTTTGCAG AAGACCCCTC CCAGTTGCCT TCAACTCAGT 541 TACCTTCCTC ACCTTTTGGA GACTATCGAC AATATCAGTA CCCAACTTTC TTGTACAAAG 601 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 661 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 721 ACGAATGACT GTGTTAGGCG GCTCACGGAC GCGTTAAGTC gacaatcaac ctctggatta 781 caaaatttgt gaaagatt