Construct: ORF TRCN0000472006
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000163.1_s317c1
- Derived from:
- ccsbBroadEn_04475
- DNA Barcode:
- TAGTACCAAAAGCTTAACAGCGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATCAY (85300)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472006
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 85300 | ATCAY | ATCAY kinesin light chain i... | NM_033064.5 | 100% | 100% | |
| 2 | mouse | 16467 | Atcay | ataxia, cerebellar, Cayman ... | NM_178662.3 | 83.5% | 91.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1179
- ORF length:
- 1113
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gaccaccgaa gccacgctcc ggatggaaaa cgtggacgtg aaggaggaat 121 ggcaggacga agatcttccc aggccactcc cagaagagac gggggtggaa ctgcttggca 181 gcccggtgga agacacatcc tctcctccca acacgctaaa tttcaacgga gcgcatcgta 241 agaggaagac gctggtggcc ccagagatca acatttctct ggatcagagt gaggggtccc 301 tgctgtccga tgacttcttg gatacccctg atgacctgga tattaacgtg gatgacatcg 361 agacccccga tgagaccgac tcgctggagt tcctggggaa tggcaacgaa ctggagtggg 421 aagacgacac ccccgtggcc accgccaaga acatgcccgg ggacagcgcg gatctatttg 481 gggacggcac gacggaggac ggcagcgccg ccaacgggcg cctgtggcgg acagtgatca 541 tcggggagca agagcaccgt atagacctgc acatgatccg gccttacatg aaagtggtca 601 cccacggagg gtactacggc gaaggcctca acgccatcat cgtcttcgca gcctgcttcc 661 ttccagacag cagcctcccc gactaccact acatcatgga gaacctcttc ctgtacgtca 721 tcagcagctt agagctcctg gtggctgagg actacatgat cgtgtacctg aaCGGTGCCA 781 CGCCCCGGCG GAGGATGCCT GGAATCGGCT GGCTGAAGAA GTGCTACCAG ATGATCGACC 841 GGAGGTTGCG GAAAAACCTG AAGTCCTTGA TCATCGTCCA CCCCTCGTGG TTCATTCGGA 901 CTGTGCTGGC CATCTCTCGC CCTTTCATCA GCGTCAAGTT CATCAACAAG ATCCAGTACG 961 TGCACAGCTT GGAAGACCTG GAGCAACTCA TCCCTATGGA ACACGTCCAG ATCCCAGACT 1021 GCGTCCTGCA ATACGAAGAG GAAAGACTGA AGGCCAGGAG GGAGAGCGCG AGGCCCCAGC 1081 CGGAGTTTGT GCTGCCCAGG TCTGAAGAGA AGCCAGAGGT GGCACCAGTG GAAAACAGGT 1141 CTGCTCTGGT CTCAGAAGAT CAGGAAACAA GCATGTCCTG CCCAACTTTC TTGTACAAAG 1201 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1261 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1321 ACGATAGTAC CAAAAGCTTA ACAGCGTTAC GCGTTAAGTC gacaatcaac ctctggatta 1381 caaaatttgt gaaagatt